Abstract
Purpose:The aim of this prospective, randomized study was to compare the results obtained in ICSI with two culture media, P-1 (Irvine Scientific) and IVF-50 (Scandinavian IVF Science).
Methods: A total of 182 patients undergoing ICSI treatment were randomly included in this study and divided in two groups: Group I: P-1 medium (n = 91) or Group II: IVF-50 medium (n = 91). All the embryos were transferred on the second day.
Results: Patient age did not differ (p = .29) between Group I (34.8 ± 4.8) and Group II (34.0 ± 4.5). The number of oocytes retrieved from Group I (10.6 ± 6.7) was also similar (p = .49) to that retrieved from Group II (11.1 ± 6.4). In addition, there was no difference (p = .25) in the number of oocytes retrieved at metaphase II between Group I (7.9 ± 4.6) and Group II (8.7 ± 4.6). Normal fertilization rates, abnormal fertilization rates, and cleavage rates were similar (p = .62, p = .48, and p = .9, respectively) between Group I (68.4 ± 23.3%, 6.7 ± 10.3%, and 98.7 ±4.6%) and Group II (65.3 ± 26.2%, 9.0 ± 13.8%, and 98.9 ± 3.9%, respectively). The embryo score was also similar (p = .62) for both groups (Group I: 31.9 ± 14.0 and Group II: 33.4 ± 15.8). There was no difference in the number of embryos transferred (p = .69) between Group I (2.8 ± 1.0) and Group II (2.8 ± 1.1). In addition, pregnancy rates/puncture, pregnancy rates/transfer, implantation rates, and abortion rates were also similar for Group I (36.2%, 37.0%, 17.4%, and 12.1%, respectively) and Group II (31.8%, 33.7%, 15.8%, and 10.3%, respectively) (p = .64, p = .75, p = .72, and p = 1.0, respectively).
Conclusions: There were no differences in the results obtained with culture media P-1 (Irvine Scientific) and IVF-50 (Scandinavian IVF Science) for ICSI and embryo culture.
Keywords: culture medium, embryo implantation, ICSI
Full Text
The Full Text of this article is available as a PDF (50.8 KB).
REFERENCES
- 1.Bavister BD. Culture of preimplantation embryos: Facts and anti facts. Hum Reprod Update. 1995;1:91–148. doi: 10.1093/humupd/1.2.91. [DOI] [PubMed] [Google Scholar]
- 2.Franco JG, Jr, Baruffi RLR, Coelho J, Mauri AL, Petersen CG, Garbellini E. A prospective and randomized study of ovarian stimulation for ICSI with recombinant FSH versus highly purified urinary FSH. Gynecol Endocrinol. 2000;14:5–10. doi: 10.3109/09513590009167653. [DOI] [PubMed] [Google Scholar]
- 3.Oliveira JBA, Baruffi RLR, Mauri AL, Petersen CG, Borges MC, Franco JG., Jr Endometrial ultrasonography as a predictor of pregnancy in an in-vitro fertilization programme after ovarian stimulation and gonadotrophin-releasing hormone and gonadotrophins. Hum Reprod. 1997;12:2515–2518. doi: 10.1093/humrep/12.11.2515. [DOI] [PubMed] [Google Scholar]
- 4.Svalander P, Forsberg AS, Jakobsson AH, Wikland M. Factors of importance for the establishment of a successful program of intracytoplasmic sperm injection treatment for male infertility. Fertil Steril. 1995;63:828–837. doi: 10.1016/s0015-0282(16)57489-5. [DOI] [PubMed] [Google Scholar]
- 5.Karamelegos C, Bolton VN. A prospective comparison of “in house” and commercially prepared Earle's balanced salt soltion in human in-vitro fertilization. Hum Reprod. 1999;14:1842–1846. doi: 10.1093/humrep/14.7.1842. [DOI] [PubMed] [Google Scholar]
- 6.Staessen C, Janssenwillen C, De Clerck E, Van Steirteghem A. Controlled comparison of commercial media for human in-vitro fertilization: Ménézo B2 medium versus Medi-Cult universal and BM1 medium. Hum Reprod. 1998;13:2548–2554. doi: 10.1093/humrep/13.9.2548. [DOI] [PubMed] [Google Scholar]
- 7.Parinaud J, Veiga A, Vieitez G, Busquets A, Milhet P, Calderon G, Richoilley G, Barri P. Randomized comparison of three media used for embryo culture after intracytoplasmic sperm injection. Hum Reprod. 1998;13(Suppl4):212–217. doi: 10.1093/humrep/13.suppl_4.212. [DOI] [PubMed] [Google Scholar]
- 8.De Clerck E, Janssens R, Joris H, Verhein G, Van Steirteghem A. Prospective, controlled, randomized culture systems:Ménézo B2 medium and Cook IVF medium (Abstract Book) Hum Reprod. 2000;15:34. [Google Scholar]