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. 1998 Mar;24(1):1–17. doi: 10.1023/A:1005039023702

Effects of mutual influence of photoinduced electron transitions and slow structural rearrangements in bacterial photosynthetic reaction centers

Grachik A Abgaryan 1, Leonid N Christophorov 2, Alexander O Goushcha 3, Alfred R Holzwarth 4, Valery N Kharkyanen 3, Peter P Knox 5, Eugene A Lukashev 5
PMCID: PMC3455867  PMID: 23345666

Abstract

We describe the phenomenon of light-induced structural transformations in the reaction centers (RC) of photosynthetic bacteria which makes self-regulation of the RC charge separation efficiency possible. The nature of the effect is that the light-driven electron transfer (ET) between the RC redox-cofactors causes structural changes in the protein-cofactors system and this in turn affects the ET kinetics. If the electron-conformation interaction is strong enough, then such self-regulation gives birth to a new RC conformational state of enhanced charge separation efficiency. We show experimental results of stationary and kinetic absorbance change characteristics under different photoexcitation conditions, indicating structural rearrangements on a rather long (minutes) time scale, mainly within the secondary acceptor binding pocket. To simplify the description, in constructing a theory of structure-function reorganization in the RC we employ the adiabatic approach. Final expressions enable us to make qualitative comparison with experimentally observed kinetics of the fast and slow stages of ‘free’ and ‘structurally controlled’ electron relaxation, respectively.

Keywords: Non-linear dynamics, bistability, slow conformational mode, conformational state, light-induced changes, reaction center(s), Rhodobacter sphaeroides, electron transfer, adiabatic theory

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