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. 1984 Jul;81(14):4378–4380. doi: 10.1073/pnas.81.14.4378

Crystallization of myosin subfragment 1.

I Rayment, D A Winkelmann
PMCID: PMC345592  PMID: 6589600

Abstract

Crystals of myosin subfragment 1 from avian skeletal muscle have been grown reproducibly. They diffract x-rays to at least 4.5-A resolution. The subfragment 1 crystallizes in space group P2(1)2(1)2(1) where a = 107 A, b = 117 A, and c = 278 A. The cell dimensions and intensity distribution on x-ray diffraction photographs are consistent with two molecules in the crystallographic asymmetric unit. Electrophoretic analysis shows that the myosin subfragment 1 present in the crystals contains a 95-kilodalton heavy chain fragment and both the essential and regulatory light chains.

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Selected References

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