Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Jul;81(14):4389–4393. doi: 10.1073/pnas.81.14.4389

Structure of the cholesteryl ester core of human plasma low density lipoproteins: selective deuteration and neutron small-angle scattering.

P Laggner, G M Kostner, G Degovics, D L Worcester
PMCID: PMC345595  PMID: 6589601

Abstract

The structural arrangement of cholesteryl esters in human plasma low density lipoproteins (LDL) has been studied by selective deuteration and neutron small-angle scattering. LDL were labeled by in vitro exchange with two different kinds of deuterated cholesteryl esters, one labeled in the fatty acyl chain (cholesteryl myristate-d27) and the other in the branched side chain of cholesterol (cholesteryl-25,26,27-d7 oleate). Neutron scattering data from deuterated and protonated LDL were compared to identify the locations of the fatty acyl and cholesterol side chain moieties. Below the thermotropic transition, radii of gyration of 60 A and 70 A were obtained for these two domains, respectively, indicating that the cholesteryl nuclei are situated more distantly from the center than the fatty acyl chains. At 37 degrees C, above the thermotropic transition of the cholesteryl esters in LDL, both parts have similar radii of gyration of approximately 56 A. This information is used in a discussion of possible structural models for the apolar lipid core of LDL.

Full text

PDF
4389

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson D., Deckelbaum R. J., Small D. M., Shipley G. G. Structure of human plasma low-density lipoproteins: molecular organization of the central core. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1042–1046. doi: 10.1073/pnas.74.3.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blaurock A. E. A change observation of oriented diffraction from low-density serum lipoprotein. Biochim Biophys Acta. 1981 Jan 26;663(1):203–210. doi: 10.1016/0005-2760(81)90206-x. [DOI] [PubMed] [Google Scholar]
  3. Burks C., Engelman D. M. Cholesteryl myristate conformation in liquid crystalline mesophases determined by neutron scattering. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6863–6867. doi: 10.1073/pnas.78.11.6863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Craig I. F., Via D. P., Sherrill B. C., Sklar L. A., Mantulin W. W., Gotto A. M., Jr, Smith L. C. Incorporation of defined cholesteryl esters into lipoproteins using cholesteryl ester-rich microemulsions. J Biol Chem. 1982 Jan 10;257(1):330–335. [PubMed] [Google Scholar]
  5. Deckelbaum R. J., Shipley G. G., Small D. M., Lees R. S., George P. K. Thermal transitions in human plasma low density lipoproteins. Science. 1975 Oct 24;190(4212):392–394. doi: 10.1126/science.170681. [DOI] [PubMed] [Google Scholar]
  6. Deckelbaum R. J., Shipley G. G., Small D. M. Structure and interactions of lipids in human plasma low density lipoproteins. J Biol Chem. 1977 Jan 25;252(2):744–754. [PubMed] [Google Scholar]
  7. Goldstein J. L., Brown M. S. The low-density lipoprotein pathway and its relation to atherosclerosis. Annu Rev Biochem. 1977;46:897–930. doi: 10.1146/annurev.bi.46.070177.004341. [DOI] [PubMed] [Google Scholar]
  8. Jürgens G., Knipping G. M., Zipper P., Kayushina R., Degovics G., Laggner P. Structure of two subfractions of normal porcine (Sus domesticus) serum low-density lipoproteins. X-ray small-angle scattering studies. Biochemistry. 1981 May 26;20(11):3231–3237. doi: 10.1021/bi00514a038. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Laggner P., Degovics G., Müller K. W., Glatter O., Kratky O., Kostner G., Holasek A. Molecular packing and fluidity of lipids in human serum low density lipoproteins. Hoppe Seylers Z Physiol Chem. 1977 Jul;358(7):771–778. doi: 10.1515/bchm2.1977.358.2.771. [DOI] [PubMed] [Google Scholar]
  11. Laggner P., Kostner G. M., Rakusch U., Worcester D. Neutron small angle scattering on selectively deuterated human plasma low density lipoproteins. The location of polar phospholipid headgroups. J Biol Chem. 1981 Nov 25;256(22):11832–11839. [PubMed] [Google Scholar]
  12. Laggner P., Müller K. W. The structure of serum lipoproteins as analysed by X-ray small-angle scattering. Q Rev Biophys. 1978 Aug;11(3):371–425. doi: 10.1017/s0033583500002304. [DOI] [PubMed] [Google Scholar]
  13. Luzzati V., Tardieu A., Aggerbeck L. P. Structure of serum low-density lipoprotein. I. A solution X-ray scattering study of a hyperlipidemic monkey low-density lipoprotein. J Mol Biol. 1979 Jul 5;131(3):435–473. doi: 10.1016/0022-2836(79)90002-0. [DOI] [PubMed] [Google Scholar]
  14. Mateu L., Tardieu A., Luzzati V., Aggerbeck L., Scanu A. M. On the structure of human serum low density lipoprotein. J Mol Biol. 1972 Sep 14;70(1):105–116. doi: 10.1016/0022-2836(72)90166-0. [DOI] [PubMed] [Google Scholar]
  15. Müller K., Laggner P., Glatter O., Kostner G. The structure of human-plasma low-density lipoprotein B. An X-ray small-angle scattering study. Eur J Biochem. 1978 Jan 2;82(1):73–90. doi: 10.1111/j.1432-1033.1978.tb11998.x. [DOI] [PubMed] [Google Scholar]
  16. Pilz I., Glatter O., Kratky O. Small-angle X-ray scattering. Methods Enzymol. 1979;61:148–249. doi: 10.1016/0076-6879(79)61013-3. [DOI] [PubMed] [Google Scholar]
  17. Stuhrmann H. B., Tardieu A., Mateu L., Sardet C., Luzzati V., Aggerbeck L., Scanu A. M. Neutron scattering study of human serum low density lipoprotein. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2270–2273. doi: 10.1073/pnas.72.6.2270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tardieu A., Mateu L., Sardet C., Weiss B., Luzzati V. Structure of human serum lipoproteins in solution. II. Small-angle x-ray scattering study of HDL and LDL. J Mol Biol. 1976 Feb 25;101(2):129–153. doi: 10.1016/0022-2836(76)90368-5. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES