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. 1984 Jul;81(14):4470–4474. doi: 10.1073/pnas.81.14.4470

A selection for myosin heavy chain mutants in the nematode Caenorhabditis elegans.

P Anderson, S Brenner
PMCID: PMC345612  PMID: 6589606

Abstract

The unc-54 gene of Caenorhabditis elegans encodes an abundant myosin heavy chain protein expressed in body-wall muscle cells. We have designed genetic techniques that select directly for unc-54 mutants. This selection is based upon properties of the unc-54 dominant allele e1152. Mutations that eliminate dominance of e1152 are null alleles of unc-54. Deletions have been identified by their genetic properties. We have defined mutationally a number of essential genes near unc-54, and we have described the genetic fine structure of this region of linkage group I. As much as 27% of the unc-54 mutations induced by the bifunctional alkylating agent 1,2,7,8-diepoxyoctane are multisite deletions. Extrachromosomal free duplications that include unc-54 are also described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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