Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Jul;81(14):4520–4523. doi: 10.1073/pnas.81.14.4520

Internal complementarities in the immune system: regulation of the expression of helper T-cell idiotypes.

C Martinez, P Pereira, R Bernabé, A Bandeira, E L Larsson, P A Cazenave, A Coutinho
PMCID: PMC345622  PMID: 6235521

Abstract

More than half of BALB/c helper T lymphocytes specific for 2,4,6-trinitrophenyl (TNP)-modified syngeneic spleen cells are inhibited in their proliferative responses to antigen-presenting cells and in their cooperation with B lymphocytes by monoclonal anti-idiotypic antibodies directed to a TNP-binding BALB/c myeloma protein (MOPC 460). This inhibition is specific for anti-TNP-self helper cells of BALB/c origin and is controlled by IgCh-linked genes, as it is not observed with CB.20 helper cells of the same specificity. In contrast, anti-TNP-self helper cells prepared from BALB/c mice that were chronically suppressed with anti-mu chain antibodies and possessed no B lymphocytes were not inhibited by anti-idiotypic antibodies. We conclude that the B-cell antibody repertoires contribute to the selection of the (idiotypic) T-helper-cell repertoires.

Full text

PDF
4520

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson J., Coutinho A., Lernhardt W., Melchers F. Clonal growth and maturation to immunoglobulin secretion in vitro of every growth-inducible B lymphocyte. Cell. 1977 Jan;10(1):27–34. doi: 10.1016/0092-8674(77)90136-2. [DOI] [PubMed] [Google Scholar]
  2. Andersson J., Coutinho A., Melchers F. Frequencies of mitogen-reactive B cells in the mouse. I. Distribution in different lymphoid organs from different inbred strains of mice at different ages. J Exp Med. 1977 Jun 1;145(6):1511–1519. doi: 10.1084/jem.145.6.1511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Binz H., Wigzell H., Bazin H. T-cell idiotypes are linked to immunoglobulin heavy chain genes. Nature. 1976 Dec 16;264(5587):639–642. doi: 10.1038/264639a0. [DOI] [PubMed] [Google Scholar]
  4. Buttin G., LeGuern G., Phalente L., Lin E. C., Medrano L., Cazenave P. A. Production of hybrid lines secreting monoclonal anti-idiotypic antibodies by cell fusion on membrane filters. Curr Top Microbiol Immunol. 1978;81:27–36. doi: 10.1007/978-3-642-67448-8_4. [DOI] [PubMed] [Google Scholar]
  5. Gronowicz E., Coutinho A., Melchers F. A plaque assay for all cells secreting Ig of a given type or class. Eur J Immunol. 1976 Aug;6(8):588–590. doi: 10.1002/eji.1830060812. [DOI] [PubMed] [Google Scholar]
  6. Janeway C. A., Jr, Murgita R. A., Weinbaum F. I., Asofsky R., Wigzell H. Evidence for an immunoglobulin-dependent antigen-specific helper T cell. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4582–4586. doi: 10.1073/pnas.74.10.4582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jerne N. K., Roland J., Cazenave P. A. Recurrent idiotopes and internal images. EMBO J. 1982;1(2):243–247. doi: 10.1002/j.1460-2075.1982.tb01154.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kraig E., Kronenberg M., Kapp J. A., Pierce C. W., Abruzzini A. F., Sorensen C. M., Samelson L. E., Schwartz R. H., Hood L. E. T and B cells that recognize the same antigen do not transcribe similar heavy chain variable region gene segments. J Exp Med. 1983 Jul 1;158(1):192–209. doi: 10.1084/jem.158.1.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Krawinkel U., Cramer M., Imanishi-Kari T., Jack R. S., Rajewsky K., Mäkelä O. Isolated hapten-binding receptors of sensitized lymphocytes. I. Receptors from nylon wool-enriched mouse T lymphocytes lack serological markers of immunoglobulin constant domains but express heavy chain variable portions. Eur J Immunol. 1977 Aug;7(8):566–573. doi: 10.1002/eji.1830070814. [DOI] [PubMed] [Google Scholar]
  10. Krawinkel U., Cramer M., Kindred B., Rajewsky K. Isolated hapten-binding receptors of sensitized lymphocytes. V. Cellular origin of receptor molecules. Eur J Immunol. 1979 Oct;9(10):815–820. doi: 10.1002/eji.1830091013. [DOI] [PubMed] [Google Scholar]
  11. Layet C., Le Bouteiller P. P., Olive D., Mishal Z., Caillol D., Kourilsky F. M., Jordan B. R., Lemonnier F. A. Absence of cell surface fixation of a monoclonal antibody detectable by conventional immunoassays does not exclude expression of and interaction with the corresponding antigenic determinant. Eur J Immunol. 1984 Jan;14(1):99–102. doi: 10.1002/eji.1830140118. [DOI] [PubMed] [Google Scholar]
  12. Marx J. L. The T cell receptor--at hand at last. Science. 1983 Jul 29;221(4609):444–446. doi: 10.1126/science.6603017. [DOI] [PubMed] [Google Scholar]
  13. Rajewsky K., Eichmann K. Antigen receptors of T helper cells. Contemp Top Immunobiol. 1977;7:69–112. doi: 10.1007/978-1-4684-3054-7_3. [DOI] [PubMed] [Google Scholar]
  14. Rajewsky K., Takemori T. Genetics, expression, and function of idiotypes. Annu Rev Immunol. 1983;1:569–607. doi: 10.1146/annurev.iy.01.040183.003033. [DOI] [PubMed] [Google Scholar]
  15. Ron Y., De Baetselier P., Gordon J., Feldman M., Segal S. Defective induction of antigen-reactive proliferating T cells in B cell-deprived mice. Eur J Immunol. 1981 Dec;11(12):964–968. doi: 10.1002/eji.1830111203. [DOI] [PubMed] [Google Scholar]
  16. Sege K., Peterson P. A. Use of anti-idiotypic antibodies as cell-surface receptor probes. Proc Natl Acad Sci U S A. 1978 May;75(5):2443–2447. doi: 10.1073/pnas.75.5.2443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sy M. S., Lowy A., HayGlass K., Janeway C. A., Jr, Gurish M., Greene M. I., Benacerraf B. Chronic treatment with rabbit anti-mouse mu-chain antibody alters the characteristic immunoglobulin heavy-chain restriction of murine suppressor T-cell factors. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3846–3850. doi: 10.1073/pnas.81.12.3846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weinberger J. Z., Greene M. I., Benacerraf B., Dorf M. E. Hapten-specific T-cell responses to 4-hydroxy-3-nitrophenyl acetyl. I. Genetic control of delayed-type hypersensitivity by VH and I-A-region genes. J Exp Med. 1979 Jun 1;149(6):1336–1348. doi: 10.1084/jem.149.6.1336. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES