Abstract
Primary human keratinocytes can be driven,in vitro, to differentiate, viaactivation of transglutaminases, by raisingthe culture medium calcium concentrationabove 1 mM. This results intransglutaminase regulated cross linking ofspecific amino acids with resultantcornified envelope formation. Thedifferentiation was monitored via theincorporation of fluorescein cadaverineinto the cornified envelops. Thisdifferentiation assay was combined withassessment of reductive capacity ofresazurin, as a measure of cellactivity/viability.One primary aim is to assess the effects ofTHz radiation on human skin, since medicalimaging of the body through the skin isenvisaged.Human keratinocytes, at passage 2 fromisolation, were grown to confluence, andtransported in a buffered salt solution at22 °C. The exposure to the THz sourcewas for 10, 20 or 30 minutes at roomtemperature.No donor specific inhibition or stimulationof cell activity, compared with non-exposedcells, was noted following exposure in therange 1 to 3 THz, at up to 0.45J/cm2.The differentiation also occurred in anormal way, for exposed and non-exposedcells, with the FC incorporation increasingbetween day 3 and day 8, as previouslynoted.
Keywords: Differentiation, human primary keratinocytes, in vitro, resazurin assay, THz effects
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