Abstract
The marker-free on-chip distinction betweenhybridised (double-stranded) DNA (HDNA) anddenatured (single-stranded) DNA (DDNA) hasrecently been demonstrated using ultrashortelectrical pulses. The electrical THzpulses propagate in integrated waveguidesincorporating resonant THz structures ontowhich the genetic material is deposited.For a possible future realisation of a highthroughput array, it is crucial to optimizethe experimental parameters and theresonant structure. In this paper weperform a first numerical study ofdifferent resonator geometries and examinethe influence of critical experimentalparameters on the transmissioncharacteristics of the resonant structures.Our simulations demonstrate that the ringresonator shows a comparable performance tothe parallel-coupled resonator previouslyused in a first demonstration by Nagel andcoworkers.
Keywords: lab-on-chip, marker-free DNA testing, numerical simulations, on-chip DNA analysis, parallel-coupled filter, ring resonator, THz on-chip detection, THz spectroscopy
Full Text
The Full Text of this article is available as a PDF (127.0 KB).
References
- 1.Chee, M., Yang, R., Hubbell, E., Berno, A., Huang, X.C., Stern, D., Winkler, J., Lockhart, D.J., Morris, M.S. and Fodor, S.P.A.: Accessing Genetic Information with High-Density DNA Arrays,Science274 (1996), 610-614. [DOI] [PubMed]
- 2.Zhu, Z., Chao, J., Yu, H. and Waggoner, A.S.: Directly labeled DNA Probes using Fluorescent Nucleotides with Different Length Linkers,Nucl. Acids Res.22 (1994), 3418-3422. [DOI] [PMC free article] [PubMed]
- 3.Nagel, M., Haring Bolivar, P., Brucherseifer, M., Kurz, H., Bosserhoff, A. and Büttner, R.: Integrated THz Technology for Label-Free Genetic Diagnostics,Appl. Phys. Lett.80 (2002), 154-156.
- 4.Valdmanis, J.A. and Mourou, G.A.: Subpicosecond Electrooptic Sampling: Principles and Applications,IEEE J. Quant. Electr.22 (1986), 69-78.
- 5.Markelz, A.G., Roitberg, A. and Heilweil, E.J.: Pulsed Terahertz Spectroscopy of DNA, Bovine Serum Albumin and Collagen between 0.1 and 2.0 THz,Chem. Phys. Lett.320 (2000), 42-48.
- 6.Haring Bolivar, P.: private communication.
- 8.Brucherseifer, M., Nagel, M., Haring Bolivar, P., Kurz, H., Bosserhoff, A. and Büttner, R.: Label-Free Probing of the Binding State of DNA by Time-Domain Terahertz Sensing,Appl. Phys. Lett.77 (2000), 4049-4051. [DOI] [PubMed]