Abstract
The aim of the present study is toinvestigate the genotoxic effect of THzradiation in human peripheral bloodlymphocytes following 20 minutes exposureto 1 mW average power Free Electron Laserradiation in the frequency range 120–140GHz. For this purpose 9 healthy donors wereemployed and cytokinesis block techniquewas applied to study micronucleusfrequency and cell proliferation. Theresults obtained indicate that all theelectromagnetic conditions adopted so far do not alter the investigated parameters,suggesting absence of direct chromosomaldamage and alteration of cell cyclekinetics (two tailed paired Student's test:p> 0.05 in all cases).
Keywords: Cell proliferation, genotoxic effects, human lymphocytes, micronuclei, THz radiation
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References
- 1.Juutilainen, J. and Lang, S: Genotoxic, Carcinogenic and Teratogenic Effects of Electromagnetic Fields. Introduction and Overview,Mutation Res.387 (1997), 165-171. [DOI] [PubMed]
- 2.Fenech, M.: The Cytokinesis-Block Micronucleus Technique: A detailed Description of the Method and its Application to Genotoxicity Studies in Human population,Mutation Res.285 (1993), 35-44. [DOI] [PubMed]
- 3.Tice, R.R., Hook, G.G., Donner, M., McRee, D.I. and Guy, A.W.: Genotoxicity of Radiofrequency Signals. I. Investigation of DNA Damage and Micronuclei Induction in Cultured Human Blood Cells,Bioelectromagnetics23 (2002), 113-126. [DOI] [PubMed]
- 4.Scarfì, M.R., Lioi, M.B., Della Noce, M., Zeni, O., Franceschi, C., Monti, D., Castellani G. and Bersani, F.: Exposure to 100 Hz pulsed Magnetic Fields Increase Micronucleus Frequency and Cell Proliferation in Human Lymphocytes,Bioelectrochem. & Bioenerg.43(1) (1997), 77-81.
- 5.Gallerano, G.P. Doria, A., Giovenale, E. and Renieri, A.: Compact Free Electron Lasers: From Cerenkov to Waveguide FELs,Infrared Phys. and Tech.40 (1999), 161-174.
- 6.Giovenale, E., D'Arienzo, M., Doria, A., Gallerano, G.P., Lai, A., Messina, G. and Piccinelli, D.: Absorption and Diffusion Measurements of Biological Samples using a THz Free Electron Laser, Tu-P21 these Proceedings [DOI] [PMC free article] [PubMed]
- 7.Surralles, J., Xamena, N., Creus, A., Catalan, J., Norppa, H. and Marcos, R.: Induction of Micronuclei by Five Pyrethroid Insecticides in Whole-Blood and Isolated Human Lymphocyte Cultures,Mutation Res.341 (1995), 169-184. [DOI] [PubMed]