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. 1999 Jun;24(2-4):157–166. doi: 10.1023/A:1005135800715

Complex Formation of E. coli RNA Polymerase with Bacteriophage T2 DNA: Long-Range Effects in DNA

SG Kamzolova 1,✉, NN Ivanova 1, SS Kamzalov 1
PMCID: PMC3456593  PMID: 23345676

Abstract

Conformation behavior of phase T2 DNA in the process of its interaction with it E. coli RNA polymerase was studied using spin labeling technique. T2 DNA was modified by the spin-labeled imidazole at OH-groups of glucosylated cytidine residues. It was shown that the binding of RNA polymerase under the conditions favoring the formation of open promoter complexes induces specific conformational changes in the spin-labeled DNA. The observed conformational changes encompass not only the promoter regions of DNA which are involved in direct contacts with RNA polymerase molecules but extend over remote DNA sites (long-range effect). In relation to this effect, current theoretical models of DNA dynamics are discussed.

Keywords: conformational transition; E. coli; RNA polymerase; long-range effects; N-(2,2′,5,5′-tetramethyl-3-carboxypyrrolidine-1-oxyl)-imidazole; open promoter complex; T2 DNA

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