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. 2002 Jun;28(2):211–223. doi: 10.1023/A:1019983715589

The Lipid Organisation of the Skin Barrier: Liquid and Crystalline Domains Coexist in Lamellar Phases

J Bouwstra 1, G Gooris 2, M Ponec 3
PMCID: PMC3456653  PMID: 23345770

Abstract

The superficial layer of the skin, the stratum corneum, is the main barrier for diffusion of substances across the skin. The stratum corneum is composed of corneocytes embedded in lipid lamellae. In previous studies two lamellar phases have been identified with periodicities of 6.4 and 13.4 nm of which the 13.4 nm phase (long periodicity phase = LPP) is considered to be very important for the skin banier function. The main lipid classes in stratumcorneum are ceramides, free fatty acids and cholesterol. Until now 8 subclassesof ceramides are identified in human stratum corneum referred to as ceramide 1 to 8. Studies with mixtures prepared with isolated human ceramides revealed that cholesterol and ceramides are very important for the formation of the lamellar phases. After addition of free fatty acids the lipids are organised in an orthorhombic packing with a small proportion of lipids in a liquid phase. Our most recent results show that the presence of ceramide 1 and the formation of a liquid phase are crucial elements for the formation of the LPP. These observations and the broad-narrowbroad sequence of lipid layers in the LPP led us to propose a molecular model for this phase. This consists of one narrow central lipid layer with fluid domains with on both sides a broad layer with a crystalline structure. This model is referred to as `the sandwich model'.

Keywords: lamellar phases, skin, X-ray diffraction

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References

  • 1.Simonetti O., Hoogstraate A.J., Bialik W., Kempenaar J.A., Schrijvers A.H.G.J., Bodde H.E., Ponec M. Visualization of Diffusion Pathways across the Stratum Corneum of Native and in-vitro-Reconstructed Epidermis by Confocal Laser Scanning Microscopy. Arch. Dermatol. Res. 1995;287:465–473. doi: 10.1007/BF00373430. [DOI] [PubMed] [Google Scholar]
  • 2.Robson K.J., Stewart M.E., Michelsen S., Lazo N.D., Downing D.T. 6-Hydroxy-4-Sphingenine in Human Epidermal Ceramides. J. Lipid Res. 1994;35:2060–2068. [PubMed] [Google Scholar]
  • 3.Stuart M.E., Downing D.T. A New 6-Hydroxy-4-Sphingenine Containing Ceramide in Human Skin. J. Lip. Res. 1999;40:1434–1439. [PubMed] [Google Scholar]
  • 4.Wertz P.W., Downing D.T. Ceramides of Pig Stratum Epidermis, Structure Determination. J. Lipid Res. 1983;24:753—758. [PubMed] [Google Scholar]
  • 5.Sato J., Denda M., Nakanishi J., Nomura J., Koyama J. Cholesterol Sulfate inhibits Proteases that are Involved in Desquamation of Stratum Corneum. J. Invest. Dermatol. 1998;111:189–93. doi: 10.1046/j.1523-1747.1998.00244.x. [DOI] [PubMed] [Google Scholar]
  • 6.Motta S., Monti M., Sesana S., Mellesi L., Caputo R., Carelli S., Ghidoni R. Ceramide Composition of the Psoriatic Scale. Biochim. Biophys. Acta. 1993;1182:147–152. doi: 10.1016/0925-4439(93)90135-n. [DOI] [PubMed] [Google Scholar]
  • 7.Lavrijsen A.P.M., Bouwstra J.A., Gooris G.S., Boddé H.E., Ponec M. Reduced Skin Barrier Function Parallels Abnormal Stratum Corneum Lipid Organisation in Patients with Lamellar Ichthyosis. J. Invest. Dermatol. 1995;105:619–624. doi: 10.1111/1523-1747.ep12323752. [DOI] [PubMed] [Google Scholar]
  • 8.Pilgram G.S.K., Engelsma-van Pelt A.M., Bouwstra J.A., Koerten H.K. Electron Diffrac-tion Provides New Information on Human Stratum Corneum Lipid Organization in Relation to Depth and Temperature. J. Invest. Dermatol. 1999;133:403–409. doi: 10.1046/j.1523-1747.1999.00706.x. [DOI] [PubMed] [Google Scholar]
  • 9.Bouwstra J.A., Gooris G.S., Salomons-de Vries M.A., van der Spek J.A., Bras W. Structure of Human Stratum Corneum as a Function of Temperature and Hydration: A Wide-Angle X-Ray Diffraction Study. Int. J. Pharm. 1992;84:205–216. [Google Scholar]
  • 10.Bouwstra J.A., Gooris G.S., van der Spek J.A., Bras W. The Structure of Human Stratum Corneum as Determined by Small Angle X-Ray Scattering. J. Invest. Dermatol. 1991;96:1006–1014. doi: 10.1111/1523-1747.ep12492217. [DOI] [PubMed] [Google Scholar]
  • 11.Bouwstra, J.A., Gooris, G.S., Dubbelaar, F.E.R. and Ponec, M.: Phase Behaviour of Lipid Mixtures Based on Human Stratum Corneum Ceramides, J. Lipid Res. (2001), in press. [PubMed]
  • 12.Bouwstra J.A., Gooris G.S., Cheng K., Weerheim A., Bras W., Ponec M. Phase Behavior of Isolated Skin Lipids. J. Lipid Res. 1996;37:999–1011. [PubMed] [Google Scholar]
  • 13.Bouwstra, J.A., Gooris, G.S., Dubbelaar, F.E.R. and Ponec, M.: Phase Behaviour of Lipid Mixtures Based on Human Stratum Corneum Ceramides: The Role of Natural and Synthetic Ceramide 1, J. Invest. Dermatol. (2001), in press. [DOI] [PubMed]
  • 14.Madison K.C., Swarzendruber D.C., Wertz P.W., Downing D.T. Presence of Intact Inter-cellular Lipid Lamellae in the Upper Layers of the Stratum Corneum. J. Invest. Dermatol. 1987;88:714–718. doi: 10.1111/1523-1747.ep12470386. [DOI] [PubMed] [Google Scholar]
  • 15.Bouwstra J., Gooris G.S., Dubbelaar F.E.R., Weerheim A.M., IJzerman A.P., Ponec M. Role of Ceramide 1 in the Molecular Organization of the Stratum Corneum Lipids. J. Lipid Res. 1998;39:186–196. [PubMed] [Google Scholar]
  • 16.Bouwstra J., Pilgram G., Gooris G., Koerten H., Ponec M. New Aspects of the Skin Barrier. Skin Pharmacol. Appl. Skin Physiol. 2001;14(1):52–62. doi: 10.1159/000056391. [DOI] [PubMed] [Google Scholar]
  • 17.Gay C.L., Guy R.H., Golden G.M., Mak V.M., Francoeur M.L. Characterization of Low-Temperature (i.e., <65 degrees C) Lipid Transitions in Human Stratum Corneum. J. Invest. Dermatol. 1994;103:233–229. doi: 10.1111/1523-1747.ep12393214. [DOI] [PubMed] [Google Scholar]
  • 18.Bouwstra J.A., Gooris G.S., Dubbelaar F.E.R., Ponec M. The Lipid Organisation in the Skin Barrier. Acta Derm. Venereol. Supp. 2000;208:23–30. doi: 10.1080/000155500750042826. [DOI] [PubMed] [Google Scholar]
  • 19.Forslind B. A Domain Mosaic Model of the Skin Barrier. Acta Derm. Venerol. 1994;74:1–6. doi: 10.2340/000155557416. [DOI] [PubMed] [Google Scholar]
  • 20.Imokawa G., Abe A., Kawashima M., Hidano A. Decreased Levels of Ceramides in Stratum Corneum of Atopid Dermatitis: An Etiologic Factor in Aptopic Dry Skin? J. Invest. Dermatol. 1991;96:523–526. doi: 10.1111/1523-1747.ep12470233. [DOI] [PubMed] [Google Scholar]
  • 21.Lavrijsen A.P.M., Bouwstra J.A., Gooris G.S., Boddé H.E., Ponec M. Reduced Skin Barrier Function Parallels Abnormal Stratum Corneum Lipid Organisation in Patients with Lamellar Ichthyosis. J. Invest. Dermatol. 1995;105:619–624. doi: 10.1111/1523-1747.ep12323752. [DOI] [PubMed] [Google Scholar]
  • 22.Schreiner V., Gooris G.S., Pfeiffer S., Lanzendorfer G., Wenck H., Diembeck W., Proksch E., Bouwstra J.A. Barrier Characteristics of Different Human Skin Types Investigated with X-Ray Diffraction, Lipid Analysis and Electron Microscopy Imaging. J. Invest. Dermatol. 2000;114:654–660. doi: 10.1046/j.1523-1747.2000.00941.x. [DOI] [PubMed] [Google Scholar]
  • 23.Pilgram G.S.K., Engelsma-van Pelt A.M., Bouwstra J.A., Koerten H.K. Electron Diffrac-tion Provides New Information on Human Stratum Corneum Lipid Organization in Relation to Depth and Temperature. J. Invest. Dermatol. 1999;113:403–409. doi: 10.1046/j.1523-1747.1999.00706.x. [DOI] [PubMed] [Google Scholar]

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