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. 1982 Jan;79(1):113–117. doi: 10.1073/pnas.79.1.113

RNA intermediates in potato spindle tuber viroid replication

Robert A Owens 1, T O Diener 1
PMCID: PMC345672  PMID: 16593138

Abstract

Two double-stranded RNA intermediates of viroid replication have been isolated from potato spindle tuber viroid (PSTV)-infected tomato tissue and characterized by polyacrylamide gel electrophoresis and DNA-RNA hybridization techniques. These replicative intermediates contain monomeric circular or linear PSTV strands complexed with a multimeric complementary RNA strand. Synchronous synthesis of single-stranded PSTV is accompained by a simultaneous marked increase in double-stranded PSTV RNA; thus, in vivo precursors of the characterized double-stranded PSTV RNAs seem to be involved in PSTV replication. A “rolling circle” model for viroid replication on a circular PSTV template can accommodate the double-stranded PSTV RNA species characterized.

Keywords: complementary RNA, replication model

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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