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. 1982 Jan;79(2):606–607. doi: 10.1073/pnas.79.2.606

Allelic exclusion of immunoglobulin expression is not caused by somatic segregation

Matthias R Wabl 1, Maria Tenkhoff 1
PMCID: PMC345794  PMID: 6952212

Abstract

We have investigated the karyotype of immunoglobulin-producing cells in heterozygous animals. Using a karyotypic marker for one homolog of a chromosome carrying immunoglobulin genes, we established that immunoglobulin-producing cells are heterozygous with respect to this chromosome. Therefore, allelic exclusion of immunoglobulin expression cannot be caused by somatic chromosome segregation.

Keywords: IgD allotype, mitogenic B-cell stimulation, Robertsonian translocation on mouse chromosome 12

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buckland R. A., Fletcher J. M., Chandley C. Characterization of the domestic horse (Equus caballus) karyotype using G- and C-banding techniques. Experientia. 1976 Sep 15;32(9):1146–1149. doi: 10.1007/BF01927593. [DOI] [PubMed] [Google Scholar]
  2. Cebra J. J., Colberg J. E., Dray S. Rabbit lymphoid cells differentiated with respect to alpha-, gamma-, and mu- heavy polypeptide chains and to allotypic markers Aa1 and Aa2. J Exp Med. 1966 Mar 1;123(3):547–558. doi: 10.1084/jem.123.3.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. D'Eustachio P., Bothwell A. L., Takaro T. K., Baltimore D., Ruddle F. H. Chromosomal location of structural genes encoding murine immunoglobulin lambda light chains. Genetics of murine lambda light chains. J Exp Med. 1981 Apr 1;153(4):793–800. doi: 10.1084/jem.153.4.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Early P., Hood L. Allelic exclusion and nonproductive immunoglobulin gene rearrangements. Cell. 1981 Apr;24(1):1–3. doi: 10.1016/0092-8674(81)90492-x. [DOI] [PubMed] [Google Scholar]
  5. Gropp A., Winking H., Zech L., Müller H. Robertsonian chromosomal variation and identification of metacentric chromosomes in feral mice. Chromosoma. 1972;39(3):265–288. doi: 10.1007/BF00290787. [DOI] [PubMed] [Google Scholar]
  6. Hengartner H., Meo T., Müller E. Assignment of genes for immunoglobulin kappa and heavy chains to chromosomes 6 and 12 in mouse. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4494–4498. doi: 10.1073/pnas.75.9.4494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herzenberg L. A., Herzenberg L. A., Black S. J., Loken M. R., Okumura K., van der Loo W., Osborne B. A., Hewgill D., Goding J. W., Gutman G. Surface markers and functional relationships of cells involved in murine B-lymphocyte differentiation. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):33–45. doi: 10.1101/sqb.1977.041.01.007. [DOI] [PubMed] [Google Scholar]
  8. Loor F., Kelus A. S. Allelic exclusion in the B lineage cells of the rabbit. Eur J Immunol. 1978 May;8(5):315–324. doi: 10.1002/eji.1830080506. [DOI] [PubMed] [Google Scholar]
  9. Melchers F., Andersson J. Synthesis, surface deposition and secretion of immunoglobulin M in bone marrow-derived lymphocytes before and after mitogenic stimulation. Transplant Rev. 1973;14:76–130. doi: 10.1111/j.1600-065x.1973.tb00103.x. [DOI] [PubMed] [Google Scholar]
  10. Meo T., Johnson J., Beechey C. V., Andrews S. J., Peters J., Searle A. G. Linkage analyses of murine immunoglobulin heavy chain and serum prealbumin genes establish their location on chromosome 12 proximal to the T (5;12) 31H breakpoint in band 12F1. Proc Natl Acad Sci U S A. 1980 Jan;77(1):550–553. doi: 10.1073/pnas.77.1.550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nesbitt M. N., Francke U. A system of nomenclature for band patterns of mouse chromosomes. Chromosoma. 1973;41(2):145–158. doi: 10.1007/BF00319691. [DOI] [PubMed] [Google Scholar]
  12. Nottenburg C., Weissman I. L. Cmu gene rearrangement of mouse immunoglobulin genes in normal B cells occurs on both the expressed and nonexpressed chromosomes. Proc Natl Acad Sci U S A. 1981 Jan;78(1):484–488. doi: 10.1073/pnas.78.1.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pernis B., Chiappino G., Kelus A. S., Gell P. G. Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues. J Exp Med. 1965 Nov 1;122(5):853–876. doi: 10.1084/jem.122.5.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ponte P. A., Siekevitz M., Schwartz R. C., Gefter M. L., Sonenshein G. E. Transcription of immunoglobulin heavy-chain sequences from the excluded allele. Nature. 1981 Jun 18;291(5816):594–596. doi: 10.1038/291594a0. [DOI] [PubMed] [Google Scholar]
  15. Swan D., D'Eustachio P., Leinwand L., Seidman J., Keithley D., Ruddle F. H. Chromosomal assignment of the mouse kappa light chain genes. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2735–2739. doi: 10.1073/pnas.76.6.2735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wabl M. R., Johnson J. P., Haas I. G., Tenkhoff M., Meo T., Inan R. Simultaneous expression of mouse immunoglobulins M and D is determined by the same homolog of chromosome 12. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6793–6796. doi: 10.1073/pnas.77.11.6793. [DOI] [PMC free article] [PubMed] [Google Scholar]

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