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. 1982 Feb;79(3):754–757. doi: 10.1073/pnas.79.3.754

In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides.

R V Zackroff, W W Idler, P M Steinert, R D Goldman
PMCID: PMC345830  PMID: 6950425

Abstract

Intermediate filaments (IF) were reconstituted in vitro from bovine neurofilament triplet polypeptides. Neural IF, solubilized in either low salt or 8 M urea solution, assembled into IF when returned to near-physiological solution conditions. The 68,000-dalton component of the triplet, purified to homogeneity by preparative NaDodSO4 electrophoresis, was renatured and reassembled into short (approximatley 0.05-micrometer) approximatley 10 nm-diameter filaments. These results demonstrate that the triplet polypeptides are components of neural IF and that the 68,000-dalton polypeptide is an IF structural protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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