Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Feb;79(3):855–859. doi: 10.1073/pnas.79.3.855

Synthesis of low molecular weight heat shock peptides stimulated by ecdysterone in a cultured Drosophila cell line.

R C Ireland, E M Berger
PMCID: PMC345851  PMID: 6801663

Abstract

Treatment of Schneider's line 3 Drosophila cells with the steroid hormone ecdysterone rapidly stimulated the synthesis and accumulation of the polypeptide previously designated p7 [Berger, E. M., Ireland, R. C. & Wyss, C. (1980) Somatic Cell Genet. 6, 119-129]. In this report, p7 is identified as the 23,000-dalton heat shock polypeptide (hsp23). In addition to hsp23, the synthesis of the low molecular weight heat shock polypeptides hsp22, hsp26, and hsp27 was also stimulated by ecdysterone, although to different extents. Hybridization of a nick-translated genomic clone containing the hsp23 gene to a total RNA blot showed that ecdysterone stimulation of hsp23 synthesis was the result of an increase in the hsp23 RNA content of S3 cells. We detected no effect of the hormone on the synthesis of heat shock polypeptides hsp68, hsp70, and hsp83.

Full text

PDF
855

Images in this article

856Image
on p.856
857Image
on p.857
857Image
on p.857
858Image
on p.858
859Image
on p.859

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arrigo A. P., Fakan S., Tissières A. Localization of the heat shock-induced proteins in Drosophila melanogaster tissue culture cells. Dev Biol. 1980 Jul;78(1):86–103. doi: 10.1016/0012-1606(80)90320-6. [DOI] [PubMed] [Google Scholar]
  2. Ashburner M., Bonner J. J. The induction of gene activity in drosophilia by heat shock. Cell. 1979 Jun;17(2):241–254. doi: 10.1016/0092-8674(79)90150-8. [DOI] [PubMed] [Google Scholar]
  3. Berger E. M., Sloboda R. D., Ireland R. C. Tubulin content and synthesis in differentiating Drosophila cells in culture. Cell Motil. 1980;1(1):113–129. doi: 10.1002/cm.970010109. [DOI] [PubMed] [Google Scholar]
  4. Berger E., Ringler R., Alahiotis S., Frank M. Ecdysone-induced changes in morphology and protein synthesis in Drosophila cell cultures. Dev Biol. 1978 Feb;62(2):498–511. doi: 10.1016/0012-1606(78)90231-2. [DOI] [PubMed] [Google Scholar]
  5. Berger E., Wyss C. Acetylcholinesterase induction by beta-ecdysone in Drosophila cell lines and their hybrids. Somatic Cell Genet. 1980 Sep;6(5):631–640. doi: 10.1007/BF01538642. [DOI] [PubMed] [Google Scholar]
  6. Cherbas P., Cherbas L., Williams C. M. Induction of acetylcholinesterase activity by beta-ecdysone in a Drosophila cell line. Science. 1977 Jul 15;197(4300):275–277. doi: 10.1126/science.877552. [DOI] [PubMed] [Google Scholar]
  7. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  8. Corces V., Holmgren R., Freund R., Morimoto R., Meselson M. Four heat shock proteins of Drosophila melanogaster coded within a 12-kilobase region in chromosome subdivision 67B. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5390–5393. doi: 10.1073/pnas.77.9.5390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Courgeon A. M. Action of insect hormones at the cellular level. Morphological changes of a diploid cell line of Drosophila melanogaster, treated with ecdysone and several analogues in vitro. Exp Cell Res. 1972 Oct;74(2):327–336. doi: 10.1016/0014-4827(72)90384-9. [DOI] [PubMed] [Google Scholar]
  10. Guttman S. D., Glover C. V., Allis C. D., Gorovsky M. A. Heat shock, deciliation and release from anoxia induce the synthesis of the same set of polypeptides in starved T. pyriformis. Cell. 1980 Nov;22(1 Pt 1):299–307. doi: 10.1016/0092-8674(80)90177-4. [DOI] [PubMed] [Google Scholar]
  11. Hodgetts R. B., Sage B., O'Connor J. D. Ecdysone titers during postembryonic development of Drosophila melanogaster. Dev Biol. 1977 Oct 1;60(1):310–317. doi: 10.1016/0012-1606(77)90128-2. [DOI] [PubMed] [Google Scholar]
  12. Ingolia T. D., Craig E. A., McCarthy B. J. Sequence of three copies of the gene for the major Drosophila heat shock induced protein and their flanking regions. Cell. 1980 Oct;21(3):669–679. doi: 10.1016/0092-8674(80)90430-4. [DOI] [PubMed] [Google Scholar]
  13. Lindquist S. Translational efficiency of heat-induced messages in Drosophila melanogaster cells. J Mol Biol. 1980 Feb 25;137(2):151–158. doi: 10.1016/0022-2836(80)90322-8. [DOI] [PubMed] [Google Scholar]
  14. Lindquist S. Varying patterns of protein synthesis in Drosophila during heat shock: implications for regulation. Dev Biol. 1980 Jun 15;77(2):463–479. doi: 10.1016/0012-1606(80)90488-1. [DOI] [PubMed] [Google Scholar]
  15. Lis J. T., Prestidge L., Hogness D. S. A novel arrangement of tandemly repeated genes at a major heat shock site in D. melanogaster. Cell. 1978 Aug;14(4):901–919. doi: 10.1016/0092-8674(78)90345-8. [DOI] [PubMed] [Google Scholar]
  16. Livak K. J., Freund R., Schweber M., Wensink P. C., Meselson M. Sequence organization and transcription at two heat shock loci in Drosophila. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5613–5617. doi: 10.1073/pnas.75.11.5613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mirault M. E., Goldschmidt-Clermont M., Moran L., Arrigo A. P., Tissières A. The effect of heat shock on gene expression in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):819–827. doi: 10.1101/sqb.1978.042.01.082. [DOI] [PubMed] [Google Scholar]
  18. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  19. Rosset R. Effects of ecdysone on a Drosophila cell line. Exp Cell Res. 1978 Jan;111(1):31–36. doi: 10.1016/0014-4827(78)90233-1. [DOI] [PubMed] [Google Scholar]
  20. Rubin G. M., Hogness D. S. Effect of heat shock on the synthesis of low molecular weight RNAs in drosophilia: accumulation of a novel form of 5S RNA. Cell. 1975 Oct;6(2):207–213. doi: 10.1016/0092-8674(75)90011-2. [DOI] [PubMed] [Google Scholar]
  21. Savakis C., Demetri G., Cherbas P. Ecdysteroid-inducible polypeptides in a Drosophila cell line. Cell. 1980 Dec;22(3):665–674. doi: 10.1016/0092-8674(80)90542-5. [DOI] [PubMed] [Google Scholar]
  22. Scott M. P., Storti R. V., Pardue M. L., Rich A. Cell-free protein synthesis in lysates of Drosophila melanogaster cells. Biochemistry. 1979 Apr 17;18(8):1588–1594. doi: 10.1021/bi00575a032. [DOI] [PubMed] [Google Scholar]
  23. Spradling A., Penman S., Pardue M. L. Analysis of drosophila mRNA by in situ hybridization: sequences transcribed in normal and heat shocked cultured cells. Cell. 1975 Apr;4(4):395–404. doi: 10.1016/0092-8674(75)90160-9. [DOI] [PubMed] [Google Scholar]
  24. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wadsworth S. C., Craig E. A., McCarthy B. J. Genes for three Drosophila heat-shock-induced proteins at a single locus. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2134–2137. doi: 10.1073/pnas.77.4.2134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wyss C. TAM selection of Drosophila somatic cell hybrids. Somatic Cell Genet. 1979 Jan;5(1):29–37. doi: 10.1007/BF01538784. [DOI] [PubMed] [Google Scholar]
  27. Yund M. A., Fristrom J. W. Uptake and binding of beta-ecdysone in imaginal discs of Drosophila melanogaster. Dev Biol. 1975 Apr;43(2):287–298. doi: 10.1016/0012-1606(75)90028-7. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES