Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Feb;79(4):1220–1224. doi: 10.1073/pnas.79.4.1220

Mapping the structural genes coding for the major urinary proteins in the mouse: combined use of recombinant inbred strains and somatic cell hybrids.

K L Bennett, P A Lalley, R K Barth, N D Hastie
PMCID: PMC345933  PMID: 6951169

Abstract

We have mapped the multiple (15-25) genes coding for the hormonally regulated major urinary proteins (MUPs) of the mouse by using a cloned cDNA probe. By Southern blot analysis of DNA from Chinese hamster-mouse somatic cell hybrids, all of the MUP genes were found to be on chromosome 4. Different inbred mouse strains showed DNA polymorphism in their MUP Southern hybridization pattern. Analysis of recombinant inbred strains derived from these parent strains has shown that all the polymorphisms are linked to the MUP-a locus on chromosome 4. The combination of these mapping techniques should be applicable to many cloned DNA sequences.

Full text

PDF
1220

Images in this article

1221Image
on p.1221
1222Image
on p.1222
1222Image
on p.1222

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey D. W. Recombinant-inbred strains. An aid to finding identity, linkage, and function of histocompatibility and other genes. Transplantation. 1971 Mar;11(3):325–327. doi: 10.1097/00007890-197103000-00013. [DOI] [PubMed] [Google Scholar]
  2. Barth R. K., Gross K. W., Gremke L. C., Hastie N. D. Developmentally regulated mRNAs in mouse liver. Proc Natl Acad Sci U S A. 1982 Jan;79(2):500–504. doi: 10.1073/pnas.79.2.500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  4. Francke U., Lalley P. A., Moss W., Ivy J., Minna J. D. Gene mapping in Mus musculus by interspecific cell hybridization: assignment of the genes for tripeptidase-1 to chromosome 10, dipeptidase-2 to chromosome 18, acid phosphatase-1 to chromosome 12, and adenylate kinase-1 to chromosome 2. Cytogenet Cell Genet. 1977;19(2-3):57–84. doi: 10.1159/000130799. [DOI] [PubMed] [Google Scholar]
  5. Fritsch E. F., Lawn R. M., Maniatis T. Molecular cloning and characterization of the human beta-like globin gene cluster. Cell. 1980 Apr;19(4):959–972. doi: 10.1016/0092-8674(80)90087-2. [DOI] [PubMed] [Google Scholar]
  6. Fyrberg E. A., Kindle K. L., Davidson N., Kindle K. L. The actin genes of Drosophila: a dispersed multigene family. Cell. 1980 Feb;19(2):365–378. doi: 10.1016/0092-8674(80)90511-5. [DOI] [PubMed] [Google Scholar]
  7. Haldane J B, Waddington C H. Inbreeding and Linkage. Genetics. 1931 Jul;16(4):357–374. doi: 10.1093/genetics/16.4.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hastie N. D., Held W. A., Toole J. J. Multiple genes coding for the androgen-regulated major urinary proteins of the mouse. Cell. 1979 Jun;17(2):449–457. doi: 10.1016/0092-8674(79)90171-5. [DOI] [PubMed] [Google Scholar]
  9. Hudson D. M., Finlayson J. S., Potter M. Linkage of one component of the major urinary protein complex of mice to the brown coat color locus. Genet Res. 1967 Oct;10(2):195–198. doi: 10.1017/s0016672300010922. [DOI] [PubMed] [Google Scholar]
  10. Kedes L. H. Histone genes and histone messengers. Annu Rev Biochem. 1979;48:837–870. doi: 10.1146/annurev.bi.48.070179.004201. [DOI] [PubMed] [Google Scholar]
  11. Lalley P. A., Francke U., Minna J. D. Homologous genes for enolase, phosphogluconate dehydrogenase, phosphoglucomutase, and adenylate kinase are syntenic on mouse chromosome 4 and human chromosome 1p. Proc Natl Acad Sci U S A. 1978 May;75(5):2382–2386. doi: 10.1073/pnas.75.5.2382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lalley P. A., Minna J. D., Francke U. Conservation of autosomal gene synteny groups in mouse and man. Nature. 1978 Jul 13;274(5667):160–163. doi: 10.1038/274160a0. [DOI] [PubMed] [Google Scholar]
  13. Minna J. D., Marshall T. H., Shaffer-Berman P. V. Chinese hamster X mouse hybrid cells segregating mouse chromosomes and isozymes. Somatic Cell Genet. 1975 Oct;1(4):355–369. doi: 10.1007/BF01538667. [DOI] [PubMed] [Google Scholar]
  14. Owerbach D., Rutter W. J., Cooke N. E., Martial J. A., Shows T. B. The prolactin gene is located on chromosome 6 in humans. Science. 1981 May 15;212(4496):815–816. doi: 10.1126/science.7221563. [DOI] [PubMed] [Google Scholar]
  15. Owerbach D., Rutter W. J., Martial J. A., Baxter J. D., Shows T. B. Genes for growth hormone, chorionic somatommammotropin, and growth hormones-like gene on chromosome 17 in humans. Science. 1980 Jul 11;209(4453):289–292. doi: 10.1126/science.7384802. [DOI] [PubMed] [Google Scholar]
  16. Pellicer A., Wigler M., Axel R., Silverstein S. The transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell. 1978 May;14(1):133–141. doi: 10.1016/0092-8674(78)90308-2. [DOI] [PubMed] [Google Scholar]
  17. Potter M., Finlayson J. S., Bailey D. W., Mushinski E. B., Reamer B. L., Walters J. L. Major urinary protein and immunoglobulin allotypes of recombinant inbred mouse strains. Genet Res. 1973 Dec;22(3):325–328. doi: 10.1017/s0016672300013112. [DOI] [PubMed] [Google Scholar]
  18. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  19. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  20. Swan D., D'Eustachio P., Leinwand L., Seidman J., Keithley D., Ruddle F. H. Chromosomal assignment of the mouse kappa light chain genes. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2735–2739. doi: 10.1073/pnas.76.6.2735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Szoka P. R., Gallagher J. F., Held W. A. In vitro synthesis and characterization of precursors to the mouse major urinary proteins. J Biol Chem. 1980 Feb 25;255(4):1367–1373. [PubMed] [Google Scholar]
  22. Szoka P. R., Paigen K. Regulation of mouse major urinary protein production by the Mup-A gene. Genetics. 1978 Nov;90(3):597–612. doi: 10.1093/genetics/90.3.597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Toole J. J., Hastie N. D., Held W. A. An abundant androgen-regulated mRNA in the mouse kidney. Cell. 1979 Jun;17(2):441–448. doi: 10.1016/0092-8674(79)90170-3. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES