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. 1982 Mar;79(5):1525–1529. doi: 10.1073/pnas.79.5.1525

Thyrotropin stimulation of the ADP-ribosyltransferase activity of bovine thyroid membranes.

P Vitti, M J De Wolf, A M Acquaviva, M Epstein, L D Kohn
PMCID: PMC346007  PMID: 6280188

Abstract

Thyrotropin increases the ADP-ribosylation activity of bovine thyroid membranes. Rapid ADP-ribosylation of membrane components is followed by increasing ADP-ribosylation of components in the supernatant of the reaction mixture. One of the major membrane proteins ADP-ribosylated in the thyrotropin-stimulated reaction has an approximate molecular weight of 40,000; this same protein is also a major ADP-ribosylated product of the A promoter of cholera toxin and appears to be related to the G regulatory subunit of the adenylate cyclase complex. The ADP-ribosylated products appearing in the supernatant solution comigrate with thyrotropin and preparations of 125I-labeled alpha subunit of thyrotropin; the alpha subunit, but not the beta subunit, of thyrotropin can be ADP-ribosylated by the membrane ADP-ribosyltransferase activity. NAD can be shown to enhance the ability of thyrotropin to stimulate the adenylate cyclase activity of bovine thyroid membrane preparations and of membrane preparations of a rat thyroid tumor whose adenylate cyclase activity is otherwise unresponsive to thyrotropin. The beta subunit of thyrotropin inhibits thyrotropin stimulation of both the ADP-ribosylation and adenylate cyclase activities of the thyroid membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amir S. M., Carraway T. F., Jr, Kohn L. D., Winand R. J. The binding of thyrotropin to isolated bovine thyroid plasma membranes. J Biol Chem. 1973 Jun 10;248(11):4092–4100. [PubMed] [Google Scholar]
  2. Consiglio E., Salvatore G., Rall J. E., Kohn L. D. Thyroglobulin interactions with thyroid plasma membranes. The existence of specific receptors and their potential role. J Biol Chem. 1979 Jun 25;254(12):5065–5076. [PubMed] [Google Scholar]
  3. De Wolf M. J., Vitti P., Ambesi-Impiombato F. S., Kohn L. D. Thyroid membrane ADP ribosyltransferase activity. Stimulation by thyrotropin and activity in functioning and nonfunctioning rat thyroid cells in culture. J Biol Chem. 1981 Dec 10;256(23):12287–12296. [PubMed] [Google Scholar]
  4. Goldhammer A., Cook G. H., Wolff J. Preactivation as a determinant for the size of thyroid adenylate cyclase. J Biol Chem. 1980 Jul 25;255(14):6918–6922. [PubMed] [Google Scholar]
  5. Kohn L. D., Consiglio E., DeWolf M. J., Grollman E. F., Ledley F. D., Lee G., Morris N. P. Thyrotropin receptors and gangliosides. Adv Exp Med Biol. 1980;125:487–503. doi: 10.1007/978-1-4684-7844-0_43. [DOI] [PubMed] [Google Scholar]
  6. Kurosky A., Markel D. E., Peterson J. W., Fitch W. M. Primary structure of cholera toxin beta-chain: a glycoprotein hormone analog? Science. 1977 Jan 21;195(4275):299–301. doi: 10.1126/science.831277. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Laskey R. A., Mills A. D. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. FEBS Lett. 1977 Oct 15;82(2):314–316. doi: 10.1016/0014-5793(77)80609-1. [DOI] [PubMed] [Google Scholar]
  10. Ledley F. D., Mullin B. R., Lee G., Aloj S. M., Fishman P. H., Hunt L. T., Dayhoff M. O., Kohn L. D. Sequence similarity between cholera toxin and glycoprotein hormones: implications for structure activity relationship and mechanism of action. Biochem Biophys Res Commun. 1976 Apr 19;69(4):852–859. doi: 10.1016/0006-291x(76)90452-6. [DOI] [PubMed] [Google Scholar]
  11. Liao T. H., Hennen G., Howard S. M., Shome B., Pierce J. G. Bovine thyrotropin. Countercurrent distribution and a comparison with the isolated subunits of luteinizing hormone. J Biol Chem. 1969 Dec 10;244(23):6458–6467. [PubMed] [Google Scholar]
  12. Liao T. H., Pierce J. G. The primary structure of bovine thyrotropin. II. The amino acid sequences of the reduced, S-carboxymethyl alpha and beta chains. J Biol Chem. 1971 Feb 25;246(4):850–865. [PubMed] [Google Scholar]
  13. Macchia V., Meldolesi M. F., Mandato E. Alterations in TSH-receptor in two transplantable rat thyroid tumors. Ann Radiol (Paris) 1977 Nov-Dec;20(8):752–756. [PubMed] [Google Scholar]
  14. Moss J., Ross P. S., Agosto G., Birken S., Canfield R. E., Vaughan M. Mechanism of action of choleragen and the glycopeptide hormones: is the nicotinamide adenine dinucleotide glycohydrolase activity observed in purified hormone preparations intrinsic to the hormone? Endocrinology. 1978 Feb;102(2):415–419. doi: 10.1210/endo-102-2-415. [DOI] [PubMed] [Google Scholar]
  15. Moss J., Vaughan M. Activation of adenylate cyclase by choleragen. Annu Rev Biochem. 1979;48:581–600. doi: 10.1146/annurev.bi.48.070179.003053. [DOI] [PubMed] [Google Scholar]
  16. Ross E. M., Gilman A. G. Biochemical properties of hormone-sensitive adenylate cyclase. Annu Rev Biochem. 1980;49:533–564. doi: 10.1146/annurev.bi.49.070180.002533. [DOI] [PubMed] [Google Scholar]
  17. Spiegel A. M., Downs R. W., Jr, Aurbach G. D. Separation of a guanine nucleotide regulatory unit from the adenylate cyclase complex with GTP affinity chromatography. J Cyclic Nucleotide Res. 1979;5(1):3–17. [PubMed] [Google Scholar]
  18. Tate R. L., Schwartz H. I., Holmes J. M., Kohn L. D. Thyrotropin receptors in thyroid plasma membranes. Characteristics of thyrotropin binding and solubilization of thyrotropin receptor activity by tryptic digestion. J Biol Chem. 1975 Aug 25;250(16):6509–6515. [PubMed] [Google Scholar]
  19. Winand R. J., Kohn L. D. Relationships of thyrotropin to exophthalmic-producing substance. Purification of homogeneous glycoproteins containing both activities from [3H]-labeled pituitary extracts. J Biol Chem. 1970 Mar 10;245(5):967–975. [PubMed] [Google Scholar]
  20. Wolff J., Jones A. B. The purification of bovine thyroid plasma membranes and the properties of membrane-bound adenyl cyclase. J Biol Chem. 1971 Jun 25;246(12):3939–3947. [PubMed] [Google Scholar]
  21. Wolff J., Winand R. J., Kohn L. D. The contribution of subunits of thyroid stimulating hormone to the binding and biological activity of thyrotropin. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3460–3464. doi: 10.1073/pnas.71.9.3460. [DOI] [PMC free article] [PubMed] [Google Scholar]

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