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. Author manuscript; available in PMC: 2013 Nov 2.
Published in final edited form as: Oncogene. 2012 Jun 25;32(18):2273–2281e12. doi: 10.1038/onc.2012.253

Figure 5.

Figure 5

Effect of TIP30 knockdown on EGFR trafficking and nuclear localization inducing by EGF in A549 cells. (a) Representative images of EGFR (green) in human A549 cells. Cells were treated with 5 ng/ml EGF and 10 nM leptomycin B in DMEM for 2 hours were then stained for EGFR. Nuclei were stained by DAPI (blue). (b) Western blot analysis of EGFR nuclear localization. A549 cells were collected after EGF treatment for 3 hours. (c) Quantification of nuclear EGFR levels detected in Western blot analysis. Values are nuclear EGFR levels relative to Lamin-B levels. Data are represented as the means of three independent experiments. (d) Representative images show the localization of EGFR (red) in EEA-1 positive early endosomes (green) after 2 hours of EGF internalization. Yellow is due to overlap between red and green. Boxed areas are magnified. (e) Quantification of EGFR and EEA1 colocalization. * P < 0.05 versus control. Shown are representative of three experiments. Scale bars, 10 µm.