Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Mar;79(5):1634–1638. doi: 10.1073/pnas.79.5.1634

Human papillomavirus DNA in cutaneous primary and metastasized squamous cell carcinomas from patients with epidermodysplasia verruciformis.

R S Ostrow, M Bender, M Niimura, T Seki, M Kawashima, F Pass, A J Faras
PMCID: PMC346030  PMID: 6280194

Abstract

DNA extracted from squamous cell carcinomas from patients with the chronic wart disease syndrome, epidermodysplasia verruciformis, was analyzed for the presence of human papillomavirus (HPV)-specific DNA sequences by Southern blot hybridization analysis. Employing an HPV probe obtained by molecular cloning of viral DNA purified from benign warts from these patients, we have unequivocally identified HPV-specific nucleotide sequences in squamous cell carcinomas from these patients. Restriction endonuclease mapping indicated that the DNA present in the carcinomas was of the same type (type 5) as that found in the benign tumors from these patients and was present as unintegrated, free viral DNA. Moreover, we have demonstrated the presence of HPV-5 DNA in a subcutaneous metastatic tumor from one of these patients. This latter observation essentially eliminates the possibility that the HPV-5 DNA present in the malignant tumors in these patients resulted from cross-contamination from an adjacent benign warty lesion. In addition to wild-type HPV-5 DNA, both the primary and metastatic carcinomas analyzed also contained an HPV-5 DNA species lacking approximately 20% of the HPV-5 DNA genome. These subgenomic forms of HPV-5 DNA could not be detected in benign papillomas from these patients.

Full text

PDF
1634

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chang A. C., Nunberg J. H., Kaufman R. J., Erlich H. A., Schimke R. T., Cohen S. N. Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. Nature. 1978 Oct 19;275(5681):617–624. doi: 10.1038/275617a0. [DOI] [PubMed] [Google Scholar]
  2. Delescluse C., Prunieras M., Regnier M., Moreno G., Arouete J. Epidermodysplasia verruciformis. I. Electron microscope autoradiography and tissue culture studies. Arch Dermatol Forsch. 1972;242(2):202–215. [PubMed] [Google Scholar]
  3. Faras A. J., Krzyzek R. A., Ostrow R. S., Watts S. L., Smith D. M., Anderson D. L., Quick C. A., Pass F. Genetic variation among papillomaviruses. Ann N Y Acad Sci. 1980;354:60–79. doi: 10.1111/j.1749-6632.1980.tb27958.x. [DOI] [PubMed] [Google Scholar]
  4. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jablonska S., Dabrowski J., Jakubowicz K. Epidermodysplasia verruciformis as a model in studies on the role of papovaviruses in oncogenesis. Cancer Res. 1972 Mar;32(3):583–589. [PubMed] [Google Scholar]
  6. Jarrett W. F., McNeil P. E., Grimshaw W. T., Selman I. E., McIntyre W. I. High incidence area of cattle cancer with a possible interaction between an environmental carcinogen and a papilloma virus. Nature. 1978 Jul 20;274(5668):215–217. doi: 10.1038/274215a0. [DOI] [PubMed] [Google Scholar]
  7. Krzyzek R. A., Watts S. L., Anderson D. L., Faras A. J., Pass F. Anogenital warts contain several distinct species of human papillomavirus. J Virol. 1980 Oct;36(1):236–244. doi: 10.1128/jvi.36.1.236-244.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lancaster W. D., Olson C., Meinke W. Bovine papilloma virus: presence of virus-specific DNA sequences in naturally occurring equine tumors. Proc Natl Acad Sci U S A. 1977 Feb;74(2):524–528. doi: 10.1073/pnas.74.2.524. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Morison W. L. Viral warts, herpes simplex and herpes zoster in patients with secondary immune deficiencies and neoplasms. Br J Dermatol. 1975 Jun;92(6):625–630. doi: 10.1111/j.1365-2133.1975.tb03141.x. [DOI] [PubMed] [Google Scholar]
  11. Orth G., Jablonska S., Breitburd F., Favre M., Croissant O. The human papillomaviruses. Bull Cancer. 1978;65(2):151–164. [PubMed] [Google Scholar]
  12. Orth G., Jablonska S., Favre M., Croissant O., Jarzabek-Chorzelska M., Rzesa G. Characterization of two types of human papillomaviruses in lesions of epidermodysplasia verruciformis. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1537–1541. doi: 10.1073/pnas.75.3.1537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ostrow R. S., Krzyzek R., Pass F., Faras A. J. Identification of a novel human papilloma virus in cutaneous warts of meathandlers. Virology. 1981 Jan 15;108(1):21–27. doi: 10.1016/0042-6822(81)90524-9. [DOI] [PubMed] [Google Scholar]
  14. Rajagopalan K., Bahru J., Loo D. S., Tay C. H., Chin K. N., Tan K. K. Familial epidermodysplasia verruciformis of Lewandowsky and Lutz. Arch Dermatol. 1972 Jan;105(1):73–78. [PubMed] [Google Scholar]
  15. Reid T. M., Fraser N. G., Kernohan I. R. Generalized warts and immune deficiency. Br J Dermatol. 1976 Nov;95(5):559–564. doi: 10.1111/j.1365-2133.1976.tb00870.x. [DOI] [PubMed] [Google Scholar]
  16. Ruiter M. Malignant degeneration of skin lesions in epidermodysplasia verruciformis. Acta Derm Venereol. 1969;49(3):309–313. [PubMed] [Google Scholar]
  17. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  18. Stevens J. G., Wettstein F. O. Multiple copies of Shope virus DNA are present in cells of benign and malignant non-virus-producing neoplasms. J Virol. 1979 Jun;30(3):891–898. doi: 10.1128/jvi.30.3.891-898.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yabe Y., Okamoto T., Omori S., Tanioku K. Virus particles in epidermodysplasia verruciformis with carcinoma. Dermatologica. 1969;139(2):161–164. doi: 10.1159/000253907. [DOI] [PubMed] [Google Scholar]
  20. Yabe Y., Sadakane H. The virus of epidermodysplasia verruciformis: electron microscopic and fluorescent antibody studies. J Invest Dermatol. 1975 Sep;65(3):324–330. doi: 10.1111/1523-1747.ep12598388. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES