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. 1982 Mar;79(6):1994–1998. doi: 10.1073/pnas.79.6.1994

Structure and expression of a mouse major histocompatibility antigen gene, H-2Ld.

G A Evans, D H Margulies, R D Camerini-Otero, K Ozato, J G Seidman
PMCID: PMC346108  PMID: 6952248

Abstract

A genomic clone encoding H-2Ld, a mouse major transplantation antigen, has been identified and the structure of the H-2Ld gene has been partially determined. We isolated 35 genomic clones from a BALB/c (H-2d) genomic library by hybridization to mouse or human probes. One of these clones encodes H-2Ld as determined by two criteria. First, the gene encodes a protein that is identical at the 76 known amino acid positions for H-2Ld. Second, when introduced into L cells by DNA-mediated gene transfer, a new H-2 antigen is expressed that is recognized by anti-H-2Ld monoclonal antibodies. The sequence of the H-2Ld protein predicted by the DNA sequences shows more than 80% homology to known H-2 antigens. H-2L-like sequences are found in mutant H-2Kb molecules, suggesting that gene conversion or reciprocal recombination may play a role in the development of H-2 polymorphism.

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1994

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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