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. 1982 Apr;79(7):2236–2239. doi: 10.1073/pnas.79.7.2236

Mutant lambda phage repressor with a specific defect in its positive control function.

L Guarente, J S Nye, A Hochschild, M Ptashne
PMCID: PMC346166  PMID: 6212933

Abstract

The lambda phage repressor is both a positive and a negative regulator of gene transcription. We describe a mutant lambda phage repressor that has specifically lost its activator function. The mutant binds to the lambda phage operator sites and represses the lambda phage promoters PR and PL. However, it fails to stimulate transcription from the promoter PRM. The mutation lies in that portion of repressor--namely, the amino-terminal domain--that has been shown [Sauer, R. T., Pabo, C. O., Meyer, B. J., Ptashne, M. & Backman, K. C. (1979) Nature (London) 279, 396-400] to mediate stimulation of PRM. We suggest that the mutation has altered that region of repressor which, in the wild-type, contacts RNA polymerase to activate transcription from PRM.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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