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. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: Biochemistry. 2012 Aug 16;51(34):6847–6859. doi: 10.1021/bi300466d

Figure 6.

Figure 6

Time-resolved fluorescence anisotropy decay curves of 6-MI-containing ssDNA and dsDNA molecules. The local motion of 6-MI in the duplex containing the ATFAA sequence (TFA15ds-green) is reduced compared to the AFT07 duplex (red). In general, duplex formation (green, red) leads to slower overall motion of the DNA. The time-resolved anisotropy of ssDNA (dark green, brown) exhibits a bi-exponential decay consisting of significant local motion of 6-MI (~200ps) and overall tumbling of the DNA (~4 ns) (Table 3).