Table 4.
Equilibrium Protein-DNA Dissociation constants (Kd) measured with 6-MI containing duplexes
| DNA construct | Kd values | Kd values | Kd values | Kd values | Literature Kd |
|---|---|---|---|---|---|
| (nM)1 | (nM)2 | (nM)3 | (nM)4 | Values (nM) | |
| TFA15ds | 1.2 ± 0.8 | 3.7 ± 0.7 | 2.4 ± 0.8 | 1.2 ± 0.4 | 9±15 |
| TFA07ds | 1.5± 1.0 | ||||
| cAFTlOds | 1.1 ± 0.3 | ||||
| MSHTFA15ds | 10.2 ± 3.5 | 28±1.66 | |||
| MSH TFA09ds | 11 ± 1.9 | 13±3 | 28±1.66 | ||
| TBP TFA23ds | 10.6 ± 3.1 | 5±27 | |||
| HUTFA15ds | 4.6 ± 2.2 | 1.5±0.3 | 168 | ||
1
Measured with constant [DNA] = 2000 pM using fluorescence anisotropy.
2
Measured with constant [DNA]=1000 pM using fluorescence anisotropy.
3
Measured with constant [DNA]=500 pM using fluorescence intensity.
4
Measured with constant [DNA]=50 pM using fluorescence intensity.
5
From Vitko et al.45
6
From Zhai and Hingorani.43
7
From Perez-Howard et al.41
8
Apparent Kd measured by GMSA.37