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. 1982 Apr;79(8):2427–2431. doi: 10.1073/pnas.79.8.2427

Sequence of galR gene indicates a common evolutionary origin of lac and gal repressor in Escherichia coli.

B von Wilcken-Bergmann, B Müller-Hill
PMCID: PMC346211  PMID: 6283521

Abstract

The nucleotide sequence of the galR gene of Escherichia coli, which codes for galactose repressor, has been determined. The subunits of gal repressor are predicted to consist of 343 residues, including the NH2-terminal methionine. Twenty-six of the predicted NH2-terminal 55 residues of gal repressor are identical to the NH2-terminal residues of lac repressor. Additional homologies appear between residues 165 and 200, between residues 235 and 255, and around residue 325.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adhya S., Miller W. Modulation of the two promoters of the galactose operon of Escherichia coli. Nature. 1979 Jun 7;279(5713):492–494. doi: 10.1038/279492a0. [DOI] [PubMed] [Google Scholar]
  2. Adler K., Beyreuther K., Fanning E., Geisler N., Gronenborn B., Klemm A., Müller-Hill B., Pfahl M., Schmitz A. How lac repressor binds to DNA. Nature. 1972 Jun 9;237(5354):322–327. doi: 10.1038/237322a0. [DOI] [PubMed] [Google Scholar]
  3. Anderson S., Gait M. J., Mayol L., Young I. G. A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2. Nucleic Acids Res. 1980 Apr 25;8(8):1731–1743. doi: 10.1093/nar/8.8.1731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BUTTIN G. M'ECANISMES R'EGULATEURS DANS LA BIOSYNTH'ESE DES ENZYMES DU M'ETABOLISME DU GALACTOSE CHEZ ESCHERICHIA COLI K12. I. LA BIOSYNTH'ESE INDUITE DE LA GALACTOKINASE ET L'INDUCTION SIMULTAN'EE DE LA S'EQUENCE ENZYMATIQUE. J Mol Biol. 1963 Aug;7:164–182. doi: 10.1016/s0022-2836(63)80044-3. [DOI] [PubMed] [Google Scholar]
  5. BUTTIN G. M'ECANISMES R'EGULATEURS DANS LA BIOSYNTH'ESE DES ENZYMES DU M'ETABOLISME DU GALACTOSE CHEZ ESCHERICHIA COLI K12. II. LE D'ETERMINISME G'EN'ETIQUE DE LA R'EGULATION. J Mol Biol. 1963 Aug;7:183–205. doi: 10.1016/s0022-2836(63)80045-5. [DOI] [PubMed] [Google Scholar]
  6. Beyreuther K., Adler K., Geisler N., Klemm A. The amino-acid sequence of lac repressor. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3576–3580. doi: 10.1073/pnas.70.12.3576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  8. Buck F., Rüterjans H., Beyreuther K. 1H NMR study of the lactose repressor from Escherichia coli. FEBS Lett. 1978 Dec 15;96(2):335–338. doi: 10.1016/0014-5793(78)80430-x. [DOI] [PubMed] [Google Scholar]
  9. Büchel D. E., Gronenborn B., Müller-Hill B. Sequence of the lactose permease gene. Nature. 1980 Feb 7;283(5747):541–545. doi: 10.1038/283541a0. [DOI] [PubMed] [Google Scholar]
  10. Chou P. Y., Fasman G. D. Prediction of protein conformation. Biochemistry. 1974 Jan 15;13(2):222–245. doi: 10.1021/bi00699a002. [DOI] [PubMed] [Google Scholar]
  11. Dunaway M., Manly S. P., Matthews K. S. Model for lactose repressor protein and its interaction with ligands. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7181–7185. doi: 10.1073/pnas.77.12.7181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dunaway M., Matthews K. S. Hybrid tetramers of native and core lactose repressor protein. Assessment of operator and nonspecific DNA binding parameters and their relationship. J Biol Chem. 1980 Nov 10;255(21):10120–10127. [PubMed] [Google Scholar]
  13. Farabaugh P. J. Sequence of the lacI gene. Nature. 1978 Aug 24;274(5673):765–769. doi: 10.1038/274765a0. [DOI] [PubMed] [Google Scholar]
  14. Gronenborn B., Messing J. Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites. Nature. 1978 Mar 23;272(5651):375–377. doi: 10.1038/272375a0. [DOI] [PubMed] [Google Scholar]
  15. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  16. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Messing J., Gronenborn B., Müller-Hill B., Hans Hopschneider P. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3642–3646. doi: 10.1073/pnas.74.9.3642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Musso R., Di Lauro R., Rosenberg M., de Crombrugghe B. Nucleotide sequence of the operator-promoter region of the galactose operon of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Jan;74(1):106–110. doi: 10.1073/pnas.74.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Müller-Hill B., Crapo L., Gilbert W. Mutants that make more lac repressor. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1259–1264. doi: 10.1073/pnas.59.4.1259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Müller-Hill B. Lac repressor and lac operator. Prog Biophys Mol Biol. 1975;30(2-3):227–252. doi: 10.1016/0079-6107(76)90011-0. [DOI] [PubMed] [Google Scholar]
  22. O'Gorman R. B., Dunaway M., Matthews K. S. DNA binding characteristics of lactose repressor and the trypsin-resistant core repressor. J Biol Chem. 1980 Nov 10;255(21):10100–10106. [PubMed] [Google Scholar]
  23. Ogata R. T., Gilbert W. An amino-terminal fragment of lac repressor binds specifically to lac operator. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5851–5854. doi: 10.1073/pnas.75.12.5851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Riggs A. D., Newby R. F., Bourgeois S. lac repressor--operator interaction. II. Effect of galactosides and other ligands. J Mol Biol. 1970 Jul 28;51(2):303–314. doi: 10.1016/0022-2836(70)90144-0. [DOI] [PubMed] [Google Scholar]
  25. Riley M., Anilionis A. Evolution of the bacterial genome. Annu Rev Microbiol. 1978;32:519–560. doi: 10.1146/annurev.mi.32.100178.002511. [DOI] [PubMed] [Google Scholar]
  26. Rothstein R. J., Lau L. F., Bahl C. P., Narang S. A., Wu R. Synthetic adaptors for cloning DNA. Methods Enzymol. 1979;68:98–109. doi: 10.1016/0076-6879(79)68009-6. [DOI] [PubMed] [Google Scholar]
  27. Saedler H., Gullon A., Fiethen L., Starlinger P. Negative control of the galactose operon in E. coli. Mol Gen Genet. 1968;102(1):79–88. doi: 10.1007/BF00341872. [DOI] [PubMed] [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sauer R. T., Pabo C. O., Meyer B. J., Ptashne M., Backman K. C. Regulatory functions of the lambda repressor reside in the amino-terminal domain. Nature. 1979 May 31;279(5712):396–400. doi: 10.1038/279396a0. [DOI] [PubMed] [Google Scholar]
  30. Sauer R. T., Pan J., Hopper P., Hehir K., Brown J., Poteete A. R. Primary structure of the phage P22 repressor and its gene c2. Biochemistry. 1981 Jun 9;20(12):3591–3598. doi: 10.1021/bi00515a044. [DOI] [PubMed] [Google Scholar]
  31. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Tait R. C., Rodriguez R. L., West R. W., Jr The rapid purification of T4 DNA ligase from a lambda T4 lig lysogen. J Biol Chem. 1980 Feb 10;255(3):813–815. [PubMed] [Google Scholar]
  33. WILLSON C., PERRIN D., COHN M., JACOB F., MONOD J. NON-INDUCIBLE MUTANTS OF THE REGULATOR GENE IN THE "LACTOSE" SYSTEM OF ESCHERICHIA COLI. J Mol Biol. 1964 Apr;8:582–592. doi: 10.1016/s0022-2836(64)80013-9. [DOI] [PubMed] [Google Scholar]
  34. Weber K., Platt T., Ganem D., Miller J. H. Altered sequences changing the operator-binding properties of the Lac repressor: colinearity of the repressor protein with the i-gene map. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3624–3628. doi: 10.1073/pnas.69.12.3624. [DOI] [PMC free article] [PubMed] [Google Scholar]

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