Abstract
By using a technique for concentrating insulin 100-fold from tissue extracts with 75-95% recoveries, we earlier failed to detect pork-like insulin in guinea pig tissues and thus were unable to confirm reports from the National Institutes of Health that these tissues contain a pork-like insulin at concentrations averaging 1 ng/g. This difference could have been due to differences in strains of guinea pigs studied or in the species specificities of the antisera used for radioimmunoassay. In the current study, tissue extracts from both NIH and Hartley guinea pigs were assayed with three antisera routinely used in our laboratory and one antiserum that had been used in the National Institutes of Health laboratory. We observed that pork-like insulin in tissues from both strains of guinea pigs as determined with the four antisera is less than 0.02 ng/g. We therefore conclude that is is unlikely that nonpancreatic guinea pig tissues contain or synthesize a peptide resembling pork or other non-guinea pig mammalian insulin.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bauman W. A., Hatcher V. B., Levitt M. A., Yalow R. S. Evidence against insulin synthesis BY IM-9 lymphocytes. Diabetes. 1982 Feb;31(2):182–184. doi: 10.2337/diab.31.2.182. [DOI] [PubMed] [Google Scholar]
- Eng J., Yalow R. S. Evidence against extrapancreatic insulin synthesis. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4576–4578. doi: 10.1073/pnas.78.7.4576. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eng J., Yalow R. S. Insulin recoverable from tissues. Diabetes. 1980 Feb;29(2):105–109. doi: 10.2337/diab.29.2.105. [DOI] [PubMed] [Google Scholar]
- Havrankova J., Schmechel D., Roth J., Brownstein M. Identification of insulin in rat brain. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5737–5741. doi: 10.1073/pnas.75.11.5737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LeRoith D., Shiloach J., Roth J., Lesniak M. A. Insulin or a closely related molecule is native to Escherichia coli. J Biol Chem. 1981 Jul 10;256(13):6533–6536. [PubMed] [Google Scholar]
- Rosenzweig J. L., Havrankova J., Lesniak M. A., Brownstein M., Roth J. Insulin is ubiquitous in extrapancreatic tissues of rats and humans. Proc Natl Acad Sci U S A. 1980 Jan;77(1):572–576. doi: 10.1073/pnas.77.1.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenzweig J. L., Lesniak M. A., Samuels B. E., Yip C. C., Zimmerman A. E., Roth J. Insulin in the extrapancreatic tissues of guinea pigs differs markedly from the insulin in their pancreas and plasma. Trans Assoc Am Physicians. 1980;93:263–278. [PubMed] [Google Scholar]
- Yalow R. S., Eng J. Peptide hormones in strange places--are they there? Peptides. 1981;2 (Suppl 2):17–23. doi: 10.1016/0196-9781(81)90004-8. [DOI] [PubMed] [Google Scholar]