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. 1982 Apr;79(8):2690–2694. doi: 10.1073/pnas.79.8.2690

Light-dependent effects of a hydrolysis-resistant analog of GTP on rod photoresponses in the toad retina.

J W Clack, B Oakley 2nd, D R Pepperberg
PMCID: PMC346267  PMID: 6806815

Abstract

Responses to 100-ms flashes were recorded intracellularly from dark- and light-adapted rod photoreceptors in the isolated retina of the toad, Bufo marinus. Properties of photoresponses were analyzed under each condition of adaptation when retinas were superfused with 1.0 mM guanosine 5'-[beta, gamma-methylene]triphosphate (p[CH2]ppG), a hydrolysis-resistant analog od GTP. When applied to retinas that previously had been subjected to intense light (approximately 30% bleach), p[CH2]ppG increased both the amplitude and duration of photoresponses. By contrast, treatment of dark-adapted retinas with p[CH2]ppG did not alter these response parameters. When similarly applied to either dark- or light-adapted retinas, GTP had no effects on amplitude or duration of photoresponses. These results are discussed in terms of GTP-dependent mechanisms for rod adaptation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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