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. 1982 May;79(9):2753–2757. doi: 10.1073/pnas.79.9.2753

beta-globin transcript found in induced murine erythroleukemia cells is homologous to the beta h0 and beta h1 genes.

B A Brown, R W Padgett, S C Hardies, C A Hutchison 3rd, M H Edgell
PMCID: PMC346284  PMID: 6953428

Abstract

RNA transcripts complementary to at least one of the four beta-globin homologous genes (beta h) are found in high concentration in the murine erythroleukemic (MEL) cell line GM979 after butyric acid induction. Hybridization data indicate that the gene expressed is Hbb-beta h0 or Hbb-beta h1, or both. The level of beta h0/1 transcripts in the MEL cell is similar to the level of adult transcripts. The Hbb-beta h0/1 transcript is about 800 nucleotides in length. In addition, there is a larger beta h0/1 transcript of the same size and relative intensity as the adult beta-globin precursor. We also report significant levels of embryonic gene Hbb-y transcripts in induced GM979 cells. We have determined that the GM979 cell line has the [Hbb]s haplotype on the basis of an examination of its globin DNA restriction pattern. An additional MEL cell line of haplotype [Hbb]d (DBA/2 line 6A11A) was examined and found to contain no significant level of Hbb-beta h0, Hbb-beta h1, Hbb-beta h2, or Hbb-y gene transcripts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Affara N. A., Goldfarb P. S., Vass K., Lyons A., Harrison P. R. Cloning of a new mouse foetal beta-globin mRNA sequence. Nucleic Acids Res. 1981 Jul 10;9(13):3061–3075. doi: 10.1093/nar/9.13.3061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alter B. P., Goff S. C. Globin synthesis in mouse erythroleukemia cells in vitro: a switch in beta chains due to inducing agent. Blood. 1977 Nov;50(5):867–876. [PubMed] [Google Scholar]
  3. Alter B. P., Goff S. C. Variable globin chain synthesis in mouse erythroleukemia cells. Blood. 1978 Nov;52(5):1047–1057. [PubMed] [Google Scholar]
  4. Barker J. E. Development of the mouse hematopoietic system. I. Types of hemoglobin produced in embryonic yolk sac and liver. Dev Biol. 1968 Jul;18(1):14–29. doi: 10.1016/0012-1606(68)90020-1. [DOI] [PubMed] [Google Scholar]
  5. Blin N., Stafford D. W. A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 1976 Sep;3(9):2303–2308. doi: 10.1093/nar/3.9.2303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CRAIG M. L., RUSSELL E. S. A DEVELOPMENTAL CHANGE IN HEMOGLOBINS CORRELATED WITH AN EMBRYONIC RED CELL POPULATION IN THE MOUSE. Dev Biol. 1964 Oct;10:191–201. doi: 10.1016/0012-1606(64)90040-5. [DOI] [PubMed] [Google Scholar]
  7. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  8. Fantoni A., Bank A., Marks P. A. Globin composition and synthesis of hemoglobins in developing fetal mice erythroid cells. Science. 1967 Sep 15;157(3794):1327–1329. doi: 10.1126/science.157.3794.1327. [DOI] [PubMed] [Google Scholar]
  9. Fantoni A., Bozzoni I., Ullu E., Farace M. G. Construction of a recombinant bacterial plasmid containing DNA sequences for a mouse embryonic globin chain. Nucleic Acids Res. 1979 Aug 10;6(11):3505–3517. doi: 10.1093/nar/6.11.3505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Friend C., Patuleia M. C., De Harven E. Erythrocytic maturation in vitro of murine (Friend) virus-induced leukemic cells. Natl Cancer Inst Monogr. 1966 Sep;22:505–522. [PubMed] [Google Scholar]
  11. Friend C., Preisler H. D., Scher W. Studies on the control of differentiation of murine virus-induced erythroleukemic cells. Curr Top Dev Biol. 1974;8:81–101. doi: 10.1016/s0070-2153(08)60606-7. [DOI] [PubMed] [Google Scholar]
  12. Gilman J. G., Smithies O. Fetal hemoglobin variants in mice. Science. 1968 May 24;160(3830):885–886. doi: 10.1126/science.160.3830.885. [DOI] [PubMed] [Google Scholar]
  13. Hansen J. N., Konkel D. A., Leder P. The sequence of a mouse embryonic beta-globin gene. Evolution of the gene and its signal region. J Biol Chem. 1982 Jan 25;257(2):1048–1052. [PubMed] [Google Scholar]
  14. Ikawa Y., Aida M., Inoue Y. Isolation and characterization of high and low differentiation-inducible Friend leukemia lines. Gan. 1976 Oct;67(5):767–770. [PubMed] [Google Scholar]
  15. Jahn C. L., Hutchison C. A., 3rd, Phillips S. J., Weaver S., Haigwood N. L., Voliva C. F., Edgell M. H. DNA sequence organization of the beta-globin complex in the BALB/c mouse. Cell. 1980 Aug;21(1):159–168. doi: 10.1016/0092-8674(80)90123-3. [DOI] [PubMed] [Google Scholar]
  16. Kabat D., Sherton C. C., Evans L. H., Bigley R., Koler R. D. Synthesis of erythrocyte-specific proteins in cultured friend leukemia cells. Cell. 1975 Jul;5(3):331–338. doi: 10.1016/0092-8674(75)90109-9. [DOI] [PubMed] [Google Scholar]
  17. Konkel D. A., Maizel J. V., Jr, Leder P. The evolution and sequence comparison of two recently diverged mouse chromosomal beta--globin genes. Cell. 1979 Nov;18(3):865–873. doi: 10.1016/0092-8674(79)90138-7. [DOI] [PubMed] [Google Scholar]
  18. Konkel D. A., Tilghman S. M., Leder P. The sequence of the chromosomal mouse beta-globin major gene: homologies in capping, splicing and poly(A) sites. Cell. 1978 Dec;15(4):1125–1132. doi: 10.1016/0092-8674(78)90040-5. [DOI] [PubMed] [Google Scholar]
  19. Kraus L. M., Rasad A., Oba Y., Patterson M. T. Mouse fetal hemoglobin. Ann N Y Acad Sci. 1974 Nov 29;241(0):683–690. doi: 10.1111/j.1749-6632.1974.tb21923.x. [DOI] [PubMed] [Google Scholar]
  20. Leder P., Hansen J. N., Konkel D., Leder A., Nishioka Y., Talkington C. Mouse globin system: a functional and evolutionary analysis. Science. 1980 Sep 19;209(4463):1336–1342. doi: 10.1126/science.7414319. [DOI] [PubMed] [Google Scholar]
  21. Lienhard G. E., Secemski I. I., Koehler K. A., Lindquist R. N. Enzymatic catalysis and the transition state theory of reaction rates: transition state analogs. Cold Spring Harb Symp Quant Biol. 1972;36:45–51. doi: 10.1101/sqb.1972.036.01.009. [DOI] [PubMed] [Google Scholar]
  22. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Marks P. A., Rifkind R. A. Erythroleukemic differentiation. Annu Rev Biochem. 1978;47:419–448. doi: 10.1146/annurev.bi.47.070178.002223. [DOI] [PubMed] [Google Scholar]
  24. Miller D. M., Turner P., Nienhuis A. W., Axelrod D. E., Gopalakrishnan T. V. Active conformation of the globin genes in uninduced and induced mouse erythroleukemia cells. Cell. 1978 Jul;14(3):511–521. doi: 10.1016/0092-8674(78)90237-4. [DOI] [PubMed] [Google Scholar]
  25. Paigen K. Acid hydrolases as models of genetic control. Annu Rev Genet. 1979;13:417–466. doi: 10.1146/annurev.ge.13.120179.002221. [DOI] [PubMed] [Google Scholar]
  26. Rave N., Crkvenjakov R., Boedtker H. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels. Nucleic Acids Res. 1979 Aug 10;6(11):3559–3567. doi: 10.1093/nar/6.11.3559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Rougeon F., Mach B. Cloning and amplification of alpha and beta mouse globin gene sequences synthesised in vitro. Gene. 1977 May;1(3-4):229–239. doi: 10.1016/0378-1119(77)90047-6. [DOI] [PubMed] [Google Scholar]
  28. Russell L. B., Russell W. L., Popp R. A., Vaughan C., Jacobson K. B. Radiation-induced mutations at mouse hemoglobin loci. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2843–2846. doi: 10.1073/pnas.73.8.2843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Seeburg P. H., Shine J., Martial J. A., Ullrich A., Baxter J. D., Goodman H. M. Nucleotide sequence of part of the gene for human chorionic somatomammotropin: purification of DNA complementary to predominant mRNA species. Cell. 1977 Sep;12(1):157–165. doi: 10.1016/0092-8674(77)90193-3. [DOI] [PubMed] [Google Scholar]
  30. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  31. Stern R. H., Russell E. S., Taylor B. A. Strain distribution and linkage relationship of a mouse embryonic hemoglobin variant. Biochem Genet. 1976 Apr;14(3-4):373–381. doi: 10.1007/BF00484775. [DOI] [PubMed] [Google Scholar]
  32. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Weaver S., Comer M. B., Jahn C. L., Hutchison C. A., 3rd, Edgell M. H. The adult beta-globin genes of the "single" type mouse C57BL. Cell. 1981 May;24(2):403–411. doi: 10.1016/0092-8674(81)90330-5. [DOI] [PubMed] [Google Scholar]
  34. Weaver S., Haigwood N. L., Hutchison C. A., 3rd, Edgell M. H. DNA fragments of the Mus musculus beta globin haplotypes Hbbs and Hbbd. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1385–1389. doi: 10.1073/pnas.76.3.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Whitney J. B., 3rd Differential control of the synthesis of two hemoglobin beta chains in normal mice. Cell. 1977 Dec;12(4):863–871. doi: 10.1016/0092-8674(77)90150-7. [DOI] [PubMed] [Google Scholar]
  36. Whitney J. B., 3rd, McFarland E. C., Russell E. S. Interconversion of mouse adult hemoglobin and a neonatal spleen fast hemoglobin band. Dev Biol. 1978 Jul;65(1):233–237. doi: 10.1016/0012-1606(78)90193-8. [DOI] [PubMed] [Google Scholar]
  37. Wu N. C., Sikkema D. A., Zucker R. M. Mouse fetal hemoglobin. Biochim Biophys Acta. 1978 Sep 26;536(1):306–311. doi: 10.1016/0005-2795(78)90078-8. [DOI] [PubMed] [Google Scholar]
  38. Wu N. C., Zucker R. M. Mouse fetal hemoglobin synthesis in murine erythroleukemic cells. FEBS Lett. 1979 Mar 15;99(2):299–302. doi: 10.1016/0014-5793(79)80977-1. [DOI] [PubMed] [Google Scholar]

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