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. 1982 May;79(9):2860–2864. doi: 10.1073/pnas.79.9.2860

Preparation of a monoclonal antibody to rat liver glucose-6-phosphate dehydrogenase and the study of its immunoreactivity with native and inactivated enzyme.

M L Dao, B C Johnson, P E Hartman
PMCID: PMC346306  PMID: 6953434

Abstract

A monoclonal antibody of the IgG class was prepared against rat liver glucose-6-phosphate dehydrogenase (G6PD; D-glucose-6-phosphate:NADP+ 1-oxidoreductase, EC 1.1.1.49) by the hybridoma technique. This antibody does not affect the catalytic activity of the enzyme and shows crossreactivity with the palmitoyl CoA-inactivated G6PD. By solid phase radioimmunoassay, the presence of crossreacting materials in comparable amounts was determined in liver homogenate supernatants from rats that had been starved and refed a high-sucrose diet (containing a high level of G6PD activity) and from rats that had been starved and refed a high-fat diet (containing a low level of G6PD activity). These findings indicate that G6PD is present in an inactive form in rats fed a high-fat diet. The monoclonal antibody will facilitate isolation and characterization of the inactive variant G6PD.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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