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. 1982 May;79(9):2898–2901. doi: 10.1073/pnas.79.9.2898

F-actin in conifer roots

Thomas C Pesacreta *, William W Carley †, Watt W Webb †, Mandayam V Parthasarathy *,‡
PMCID: PMC346315  PMID: 16593185

Abstract

The distribution of F-actin in the complex tissues of a higher plant organ has been visualized by fluorescence labeling the roots of the conifers Chamaecyparis obtusa and Pseudotsuga menziesii with F-actin-specific fluorescent dye-conjugated phallicidin. F-actin is present in the parenchymatous cells of the vascular tissue. Some vascular parenchyma cells possess larger numbers of F-actin-containing structures (microfilament bundles) than are known to exist in any other higher plant cell. Tissue type appears to be an important determinant of the presence or absence of F-actin in a cell. For example, in contrast to vascular cells, cortical cells show no indication of fluorescence labeling of F-actin after incubation with fluorescent phallicidin. Cytoplasmic streaming is seen only in vascular cells and in a pattern that reflects the intracellular distribution of F-actin.

Keywords: 7-nitrobenz-2-oxa-1,3-diazole-phallicidin fluorescent labeling; cytoplasmic streaming; microfilament bundle

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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