Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 May;79(9):2947–2951. doi: 10.1073/pnas.79.9.2947

Gene amplification in Rhynchosciara salivary gland chromosomes.

D M Glover, A Zaha, A J Stocker, R V Santelli, M T Pueyo, S M De Toledo, F J Lara
PMCID: PMC346325  PMID: 6953439

Abstract

Late in the fourth larval instar, several regions of the Rhynchosciara americana salivary gland chromosomes undergo "DNA puffing." We have constructed a library of cloned cDNAs synthesized from poly(A)+RNA isolated from salivary glands during the period of development when the DNA puffs are active. From this library we have studied clones representative of three genes active during this period but not active at earlier developmental periods of the gland. One of these genes is not amplified during the developmental process and encodes a 0.6-kilobase RNA molecule. The other two genes are located within the DNA-puff sites C3 and C8 and encode 1.25-kilobase and 1.95-kilobase RNA molecules, respectively. We estimate from the quantitation of transfer hybridization experiments that each of these genes undergoes 16-fold amplification during DNA puffing.

Full text

PDF
2947

Images in this article

2947Image
on p.2947
2947Image
on p.2947
2947Image
on p.2947
2947Image
on p.2947
2948Image
on p.2948
2949Image
on p.2949
2949Image
on p.2949
2950Image
on p.2950
2950Image
on p.2950
2950Image
on p.2950

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alt F. W., Kellems R. E., Bertino J. R., Schimke R. T. Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells. J Biol Chem. 1978 Mar 10;253(5):1357–1370. [PubMed] [Google Scholar]
  2. Armelin H. A., Marques N. Transcription and processing of ribonucleic acid in Rhynchosciara salivary glands. II. Hybridization of nuclear and cytoplasmic ribonucleic acid with nuclear deoxyribonucleic acid. Indication of deoxyribonucleic acid amplification. Biochemistry. 1972 Sep 26;11(20):3672–3680. doi: 10.1021/bi00770a002. [DOI] [PubMed] [Google Scholar]
  3. BREUER M. E., PAVAN C. Behavior of polytene chromosomes of Rhynchosciara angelae at different stages of larval development. Chromosoma. 1955;7(4):341–386. [PubMed] [Google Scholar]
  4. Berendes H. D., Lara F. J. RNA synthesis: a requirement for hormone-induced DNA amplification in Rhynchosciara americana. Chromosoma. 1975;50(3):259–274. doi: 10.1007/BF00283470. [DOI] [PubMed] [Google Scholar]
  5. Bonaldo M. F., Santelli R. V., Lara F. J. The transcript from a DNA puff of Rhynchosciara and its migration to the cytoplasm. Cell. 1979 Aug;17(4):827–833. doi: 10.1016/0092-8674(79)90323-4. [DOI] [PubMed] [Google Scholar]
  6. Bostock C. J., Clark E. M. Satellite DNA in large marker chromosomes of methotrexate-resistant mouse cells. Cell. 1980 Mar;19(3):709–715. doi: 10.1016/s0092-8674(80)80047-x. [DOI] [PubMed] [Google Scholar]
  7. Crouse H. V., Keyl H. G. Extra replications in the "DNA-puffs" of Sciara coprophila. Chromosoma. 1968 Nov;25(3):357–364. doi: 10.1007/BF01183126. [DOI] [PubMed] [Google Scholar]
  8. Dolnick B. J., Berenson R. J., Bertino J. R., Kaufman R. J., Nunberg J. H., Schimke R. T. Correlation of dihydrofolate reductase elevation with gene amplification in a homogeneously staining chromosomal region in L5178Y cells. J Cell Biol. 1979 Nov;83(2 Pt 1):394–402. doi: 10.1083/jcb.83.2.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FICQ A., PAVAN C. Autoradiography of polytene chromosomes of Rhynchosciara angelae at different stages of larval development. Nature. 1957 Nov 9;180(4593):983–984. doi: 10.1038/180983a0. [DOI] [PubMed] [Google Scholar]
  10. GABRUSEWYCZ-GARCIA N. CYTOLOGICAL AND AUTORADIOGRAPHIC STUDIES IN SCIARA COPROPHILA SALIVARY GLAND CHROMOSOMES. Chromosoma. 1964 Aug 14;15:312–344. doi: 10.1007/BF00321517. [DOI] [PubMed] [Google Scholar]
  11. Gall J. G., Cohen E. H., Polan M. L. Reptitive DNA sequences in drosophila. Chromosoma. 1971;33(3):319–344. doi: 10.1007/BF00284948. [DOI] [PubMed] [Google Scholar]
  12. Gambarini A. G., Lara F. J. Under-replication of ribosomal cistrons in polytene chromosomes of Rhynchosciara. J Cell Biol. 1974 Jul;62(1):215–222. doi: 10.1083/jcb.62.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jones K. W., Bishop J. O., Brito-da-Cunha A. Complex formation between poly-r (U) and various chromosomal loci in Rhynchosciara. Chromosoma. 1973;43(4):375–390. doi: 10.1007/BF00406744. [DOI] [PubMed] [Google Scholar]
  14. Keyl H. G. A demonstrable local and geometric increase in the chromosomal DNA of Chironomus. Experientia. 1965 Apr 15;21(4):191–193. doi: 10.1007/BF02141878. [DOI] [PubMed] [Google Scholar]
  15. Kidd S. J., Glover D. M. A DNA segment from D. melanogaster which contains five tandemly repeating units homologous to the major rDNA insertion. Cell. 1980 Jan;19(1):103–119. doi: 10.1016/0092-8674(80)90392-x. [DOI] [PubMed] [Google Scholar]
  16. Kriegstein H. J., Hogness D. S. Mechanism of DNA replication in Drosophila chromosomes: structure of replication forks and evidence for bidirectionality. Proc Natl Acad Sci U S A. 1974 Jan;71(1):135–139. doi: 10.1073/pnas.71.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Laird C. D., Chool W. Y., Cohen E. H., Dickson E., Hutchinson N., Turner S. H. Organization and transcription of DNA in chromosomes and mitochondria of Drosophila. Cold Spring Harb Symp Quant Biol. 1974;38:311–327. doi: 10.1101/sqb.1974.038.01.035. [DOI] [PubMed] [Google Scholar]
  18. Lara F. J., Hollander F. M. Changes in RNA metabolism during the development of Rhynchosciara angelae. Natl Cancer Inst Monogr. 1967 Nov;27:235–242. [PubMed] [Google Scholar]
  19. Okretic M. C., Penoni J. S., Lara F. J. Messenger-like RNA synthesis and DNA chromosomal puffs in the salivary glands of Rhynchosciara americana. Arch Biochem Biophys. 1977 Jan 15;178(1):158–165. doi: 10.1016/0003-9861(77)90180-1. [DOI] [PubMed] [Google Scholar]
  20. Rudkin G. T., Corlette S. L. DISPROPORTIONATE SYNTHESIS OF DNA IN A POLYTENE CHROMOSOME REGION. Proc Natl Acad Sci U S A. 1957 Nov 15;43(11):964–968. doi: 10.1073/pnas.43.11.964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smith D. F., McClelland A., White B. N., Addison C. F., Glover D. M. The molecular cloning of a dispersed set of developmentally regulated genes which encode the major larval serum protein of D. melanogaster. Cell. 1981 Feb;23(2):441–449. doi: 10.1016/0092-8674(81)90139-2. [DOI] [PubMed] [Google Scholar]
  22. Smith D. F., Searle P. F., Williams J. G. Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA. Nucleic Acids Res. 1979 Feb;6(2):487–506. doi: 10.1093/nar/6.2.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Spradling A. C., Mahowald A. P. Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1096–1100. doi: 10.1073/pnas.77.2.1096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stuart W. D., Porter D. L. An improved in situ hybridization method. Exp Cell Res. 1978 Apr;113(1):218–222. doi: 10.1016/0014-4827(78)90105-2. [DOI] [PubMed] [Google Scholar]
  25. Twigg A. J., Sherratt D. Trans-complementable copy-number mutants of plasmid ColE1. Nature. 1980 Jan 10;283(5743):216–218. doi: 10.1038/283216a0. [DOI] [PubMed] [Google Scholar]
  26. Wahl G. M., Padgett R. A., Stark G. R. Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells. J Biol Chem. 1979 Sep 10;254(17):8679–8689. [PubMed] [Google Scholar]
  27. Winter C. E., de Bianchi A. G., Terra W. R., Lara F. J. Protein synthesis in the salivary glands of Rhynchosciara americana. Dev Biol. 1980 Mar;75(1):1–12. doi: 10.1016/0012-1606(80)90139-6. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES