Abstract
A recombinant Chinese hamster ovary clone was cultivated in a 2L Cytopilot Mini fluidized bed bioreactor using Cytoline 1 microcarriers and a 10L B. Braun stirred tank bioreactor with Cytodex 1 microcarriers. Cytoline 1 is a macroporous polyethylene microcarrier and Cytodex 1 is a solid DEAE-dextran microcarrier. Cytoline 1 microcarriers in the fluidized bed bioreactor were gently mixed by an uplifting flow. Circulation and sparging in Cytopilot Mini were separated from the fluidized microcarrier bed. Cytopilot Mini bioreactor with Cytoline 1 microcarriers offered 2.3 times more surface area than the stirred tank bioreactor. The 2L fluidized bed bioreactor accommodated approximately half the cells in the 10L stirred tank bioreactor. Moreover, Cytopilot Mini had approximately three times more product output rate and 5.5 times higher specific productivity than the stirred tank bioreactor.
Keywords: CHO, cytoline microcarrier, fluidized bed bioreactor (FBR), stirred tank bioreactor (STR)
Full Text
The Full Text of this article is available as a PDF (95.7 KB).
Footnotes
Dr. Junli Zhang
References
- Griffiths JB, Looby D, Racher AJ. Maximisation of perfusion systems and process comparison with batch-type cultures. Maximisation of perfusion cultures. Cytotechnology. 1992;9(1–3):3–9. doi: 10.1007/BF02521726. [DOI] [PubMed] [Google Scholar]
- Kratje RB, Reimann A, Hammer J, Wagner R. Cultivation of recombinant baby hamster kidney cells in a fluidized bed bioreactor system with porous borosilicate glass. Biotechnol Prog. 1994;10(4):410–420. doi: 10.1021/bp00028a011. [DOI] [PubMed] [Google Scholar]
- Lazar A, Reuveny S, Kronman C, Velan B, Shafferman A. Evaluation of anchorage-dependent cell propagation systems for production of human acetylcholinesterase by recombinant 293 cells. Cytotechnology. 1993;13(2):115–123. doi: 10.1007/BF00749938. [DOI] [PubMed] [Google Scholar]
- Mignot G, Faure T, Ganne, Arbeille B, Pavirani A, Romet-Lemonne JL. Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers. Cytotechnology. 1990;4(2):163–171. doi: 10.1007/BF00365097. [DOI] [PubMed] [Google Scholar]
- Nikolai TJ, Hu WS. Cultivation of mammalian cells on macroporous microcarriers. Enzyme Microb Technol. 1992;14(3):203–208. doi: 10.1016/0141-0229(92)90067-X. [DOI] [PubMed] [Google Scholar]
- Reiter M, Bluml G, Gaida T, Zach N, Unterluggauer F, Doblhoff-Dier O, Noe M, Plail R, Huss S, Katinger H. Modular integrated fluidized bed bioreactor technology. Bio/Technology. 1991;9:1100–1102. doi: 10.1038/nbt1191-1100. [DOI] [PubMed] [Google Scholar]
- Reiter M, Hohenwarter O, Gaida T, Zach N, Schmatz C, Bluml G, Weigang F, Nilsson K, Katinger H. The use of macroporous gelatin carriers for the cultivation of mammalian cells in fluidised bed reactors. Cytotechnology. 1990;3(3):271–277. doi: 10.1007/BF00365491. [DOI] [PubMed] [Google Scholar]
- Reiter M, Weigang F, Ernst W, Katinger HWD. High density microcarrier culture with a new device which allows oxygenation and perfusion of microcarrier cultures. Cytotechnology. 1990;3(1):39–42. doi: 10.1007/BF00365264. [DOI] [PubMed] [Google Scholar]
- Smits P, Ni J, Feng P, Wauters J, Van Hul W, Boutaibi ME, Dillon PJ, Merregaert J. The human extracellular matrix gene 1 (ECM1): genomic structure, cDNA cloning, expression pattern, and chromosomal localization. Genomics. 1997;45(3):487–495. doi: 10.1006/geno.1997.4918. [DOI] [PubMed] [Google Scholar]
- Vournakis JN, Runstadler PW., Jr. Optimization of the microenvironment for mammalian cell culture in flexible collagen microspheres in a fluidized-bed bioreactor. Bio/Technology. 1991;17:305–326. doi: 10.1016/b978-0-409-90123-8.50018-5. [DOI] [PubMed] [Google Scholar]