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. 1982 May;79(9):3024–3027. doi: 10.1073/pnas.79.9.3024

Human gamma-trace, a basic microprotein: amino acid sequence and presence in the adenohypophysis.

A Grubb, H Löfberg
PMCID: PMC346341  PMID: 6283552

Abstract

The amino acid sequence of human gamma-trace, a basic microprotein without known function, was determined by automated Edman degradations of the carboxymethylated polypeptide chain and of fragments obtained by cyanogen bromide treatment and tryptic digestion after blocking of lysine residues. The single polypeptide chain contained 120 residues, and the calculated Mr was 13,260. A proline residue at position 3 was partly hydroxylated. The presence of gamma-trace in a significant proportion of the cells in the anterior lobe of simian and human pituitary glands was demonstrated by immunohistochemical procedures with a rabbit antiserum against human gamma-trace. The tissue localization and amino acid sequence of gamma-trace indicated that this protein is connected with the peptidergic gastroenteropancreatic neuroendocrine system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUTLER E. A., FLYNN F. V. The occurrence of post-gamma protein in urine: a new protein abnormality. J Clin Pathol. 1961 Mar;14:172–178. doi: 10.1136/jcp.14.2.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Becker W. Determination of antisera titres ing the single radial immunodiffusion method. Immunochemistry. 1969 Jul;6(4):539–546. doi: 10.1016/0019-2791(69)90193-1. [DOI] [PubMed] [Google Scholar]
  3. CLAUSEN J. Proteins in normal cerebrospinal fluid not found in serum. Proc Soc Exp Biol Med. 1961 May;107:170–172. doi: 10.3181/00379727-107-26569. [DOI] [PubMed] [Google Scholar]
  4. Cejka J., Fleischmann L. E. Post- -globulin: isolation and physicochemical characterization. Arch Biochem Biophys. 1973 Jul;157(1):168–176. doi: 10.1016/0003-9861(73)90402-5. [DOI] [PubMed] [Google Scholar]
  5. Colle A., Guinet R., Leclercq M., Manuel Y. Occurrence of beta2-microglobulin and post-gamma globulin in human semen. Clin Chim Acta. 1976 Feb 16;67(1):93–97. doi: 10.1016/0009-8981(76)90221-7. [DOI] [PubMed] [Google Scholar]
  6. Dixon H. B., Perham R. N. Reversible blocking of amino groups with citraconic anhydride. Biochem J. 1968 Sep;109(2):312–314. doi: 10.1042/bj1090312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Edman P., Begg G. A protein sequenator. Eur J Biochem. 1967 Mar;1(1):80–91. doi: 10.1007/978-3-662-25813-2_14. [DOI] [PubMed] [Google Scholar]
  8. HOCHWALD G. M., THORBECKE G. J. Use of an antiserum against cerebrospinal fluid in demonstration of trace proteins in biological fluids. Proc Soc Exp Biol Med. 1962 Jan;109:91–95. doi: 10.3181/00379727-109-27113. [DOI] [PubMed] [Google Scholar]
  9. Hayashi R. Carboxypeptidase Y in sequence determination of peptides. Methods Enzymol. 1977;47:84–93. doi: 10.1016/0076-6879(77)47010-1. [DOI] [PubMed] [Google Scholar]
  10. Johansson B. G. Agarose gel electrophoresis. Scand J Clin Lab Invest Suppl. 1972;124:7–19. doi: 10.3109/00365517209102747. [DOI] [PubMed] [Google Scholar]
  11. LEE T. H., LERNER A. B., BUETTNER-JANUSCH V. On the structure of human corticotropin (adrenocorticotropic hormone). J Biol Chem. 1961 Nov;236:2970–2974. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Löfberg H., Grubb A. O. Quantitation of gamma-trace in human biological fluids: indications for production in the central nervous system. Scand J Clin Lab Invest. 1979 Nov;39(7):619–626. doi: 10.3109/00365517909108866. [DOI] [PubMed] [Google Scholar]
  14. MACPHERSON C. F., COSGROVE J. B. Immunochemical evidence for a gamma globulin peculiar to cerebrospinal fluid. Can J Biochem Physiol. 1961 Oct;39:1567–1574. doi: 10.1139/o61-170. [DOI] [PubMed] [Google Scholar]
  15. Mendez E., Lai C. Y. Reaction of peptides with fluorescamine on paper after chromatography or electrophoresis. Anal Biochem. 1975 May 12;65(1-2):281–292. doi: 10.1016/0003-2697(75)90511-4. [DOI] [PubMed] [Google Scholar]
  16. Nakanishi S., Inoue A., Kita T., Nakamura M., Chang A. C., Cohen S. N., Numa S. Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nature. 1979 Mar 29;278(5703):423–427. doi: 10.1038/278423a0. [DOI] [PubMed] [Google Scholar]
  17. Pearse A. G., Polak J. M. Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry. Histochem J. 1975 Mar;7(2):179–186. doi: 10.1007/BF01004561. [DOI] [PubMed] [Google Scholar]
  18. STEERS E., Jr, CRAVEN G. R., ANFINSEN C. B., BETHUNE J. L. EVIDENCE FOR NONIDENTICAL CHAINS IN THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12. J Biol Chem. 1965 Jun;240:2478–2484. [PubMed] [Google Scholar]
  19. Sternberger L. A., Joseph S. A. The unlabeled antibody method. Contrasting color staining of paired pituitary hormones without antibody removal. J Histochem Cytochem. 1979 Nov;27(11):1424–1429. doi: 10.1177/27.11.92498. [DOI] [PubMed] [Google Scholar]
  20. Tarr G. E., Beecher J. F., Bell M., McKean D. J. Polyquarternary amines prevent peptide loss from sequenators. Anal Biochem. 1978 Feb;84(2):622–7?0=ENG. doi: 10.1016/0003-2697(78)90086-6. [DOI] [PubMed] [Google Scholar]
  21. Thomsen J., Kristiansen K., Brunfeldt K., Sundby F. The amino acid sequence of human glucagon. FEBS Lett. 1972 Apr 1;21(3):315–319. doi: 10.1016/0014-5793(72)80192-3. [DOI] [PubMed] [Google Scholar]
  22. Tonnelle C., Colle A., Fougereau M., Manuel Y. Partial amino acid sequence of two forms of human post-gamma-globulin. Biochem Biophys Res Commun. 1979 Feb 14;86(3):613–619. doi: 10.1016/0006-291x(79)91757-1. [DOI] [PubMed] [Google Scholar]

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