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. 1982 May;79(9):3052–3056. doi: 10.1073/pnas.79.9.3052

Cyclic GMP mimics the muscarinic response in Xenopus oocytes: identity of ionic mechanisms.

N Dascal, E M Landau
PMCID: PMC346347  PMID: 6283554

Abstract

Acetylcholine (AcCho) elicits four distinct membrane responses in Xenopus oocytes; the responses can be studied by using the voltage clamp technique. The fastest of the responses, a transient inward current (D1 response), is muscarinic, being evoked by oxotremorine and blocked by atropine but not by curare or hexamethonium. The action of AcCho is cooperative, three transmitter-receptor complexes being required to cause a membrane conductance change, and the dose-response curve in most cases can be fitted by an equation assuming the existence of two binding sites with an affinity ratio of about 11. Guanosine 3',5'-cyclic monophosphate and the 8-bromo and dibutyryl derivatives cause a response similar to D1 in both its time course and the underlying ionic mechanism. The nucleotide-generated response has a smaller amplitude than the AcCho-generated D1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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