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. 1982 May;79(9):3057–3061. doi: 10.1073/pnas.79.9.3057

An 18,200-dalton adrenal protein that contains four [Met]enkephalin sequences.

D L Kilpatrick, B N Jones, R V Lewis, A S Stern, K Kojima, J E Shively, S Udenfriend
PMCID: PMC346348  PMID: 6953449

Abstract

An 18,200-dalton adrenal enkephalin-containing polypeptide was purified to homogeneity and shown to contain four internal [Met]enkephalin sequences. Protein sequence analysis and mapping of trypsin-derived peptides demonstrated that the previously described 12,600-dalton and 5,300-dalton adrenal enkephalin-containing polypeptides compose the amino-terminal and carboxyl-terminal sequences, respectively, of this larger protein. They are linked by two basic amino acid residues to form the entire sequence of the 18,200-dalton protein. This protein is the largest enkephalin-containing polypeptide yet isolated from beef adrenal medulla and serves as the precursor for several other previously characterized adrenal enkephalin-containing proteins and peptides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Böhlen P., Stein S., Stone J., Udenfriend S. Automatic Monitoring of primary amines in preparative column effluents with fluorescamine. Anal Biochem. 1975 Aug;67(2):438–445. doi: 10.1016/0003-2697(75)90316-4. [DOI] [PubMed] [Google Scholar]
  2. Gerber L. D., Stein S., Rubinstein M., Wideman J., Udenfriend S. Binding assay for opioid peptides with neuroblastoma x glioma hybrid cells: specificity of the receptor site. Brain Res. 1978 Jul 28;151(1):117–126. doi: 10.1016/0006-8993(78)90954-x. [DOI] [PubMed] [Google Scholar]
  3. Gubler U., Kilpatrick D. L., Seeburg P. H., Gage L. P., Udenfriend S. Detection and partial characterization of proenkephalin mRNA. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5484–5487. doi: 10.1073/pnas.78.9.5484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gubler U., Seeburg P., Hoffman B. J., Gage L. P., Udenfriend S. Molecular cloning establishes proenkephalin as precursor of enkephalin-containing peptides. Nature. 1982 Jan 21;295(5846):206–208. doi: 10.1038/295206a0. [DOI] [PubMed] [Google Scholar]
  5. Jones B. N., Shively J. E., Kilpatrick D. L., Kojima K., Udenfriend S. Enkephalin biosynthetic pathway: a 5300-dalton adrenal polypeptide that terminates at its COOH end with the sequence [Met]enkephalin-Arg-Gly-Leu-COOH. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1313–1315. doi: 10.1073/pnas.79.4.1313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jones B. N., Shively J. E., Kilpatrick D. L., Stern A. S., Lewis R. V., Kojima K., Udenfriend S. Adrenal opioid proteins of 8600 and 12,600 daltons: intermediates in proenkephalin processing. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2096–2100. doi: 10.1073/pnas.79.6.2096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones B. N., Stern A. S., Lewis R. V., Kimura S., Stein S., Udenfriend S., Shively J. E. Structure of two adrenal polypeptides containing multiple enkephalin sequences. Arch Biochem Biophys. 1980 Oct 1;204(1):392–395. doi: 10.1016/0003-9861(80)90048-x. [DOI] [PubMed] [Google Scholar]
  8. Kilpatrick D. L., Jones B. N., Kojima K., Udenfriend S. Identification of the octapeptide [Met]enkephalin -Arg6-Gly7-Leu8 in extracts of bovine adrenal medulla. Biochem Biophys Res Commun. 1981 Nov 30;103(2):698–705. doi: 10.1016/0006-291x(81)90506-4. [DOI] [PubMed] [Google Scholar]
  9. Kilpatrick D. L., Taniguchi T., Jones B. N., Stern A. S., Shively J. E., Hullihan J., Kimura S., Stein S., Udenfriend S. A highly potent 3200-dalton adrenal opioid peptide that contains both a [Met]- and [Leu]enkephalin sequence. Proc Natl Acad Sci U S A. 1981 May;78(5):3265–3268. doi: 10.1073/pnas.78.5.3265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kimura S., Lewis R. V., Gerber L. D., Brink L., Rubinstein M., Stein S., Udenfriend S. Purification to homogeneity of camel pituitary pro-opiocortin, the common precursor of opioid peptides and corticotropin. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1756–1759. doi: 10.1073/pnas.76.4.1756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kimura S., Lewis R. V., Stern A. S., Rossier J., Stein S., Udenfriend S. Probable precursors of [Leu]enkephalin and [Met]enkephalin in adrenal medulla: peptides of 3-5 kilodaltons. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1681–1685. doi: 10.1073/pnas.77.3.1681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lewis R. V., Fallon A., Stein S., Gibson K. D., Udenfriend S. Supports for reverse-phase high-performance liquid chromatography of large proteins. Anal Biochem. 1980 May 1;104(1):153–159. doi: 10.1016/0003-2697(80)90291-2. [DOI] [PubMed] [Google Scholar]
  13. Lewis R. V., Stern A. S., Kimura S., Rossier J., Brink L., Gerber L. D., Stein S., Udenfriend S. Opioid peptides and precursors in the adrenal medulla. Adv Biochem Psychopharmacol. 1980;22:167–179. [PubMed] [Google Scholar]
  14. Lewis R. V., Stern A. S., Kimura S., Rossier J., Stein S., Udenfriend S. An about 50,000-dalton protein in adrenal medulla: a common precursor of [Met]- and [Leu]enkephalin. Science. 1980 Jun 27;208(4451):1459–1461. doi: 10.1126/science.7384787. [DOI] [PubMed] [Google Scholar]
  15. Lewis R. V., Stern A. S., Kimura S., Stein S., Udenfriend S. Enkephalin biosynthetic pathway: proteins of 8000 and 14,000 daltons in bovine adrenal medulla. Proc Natl Acad Sci U S A. 1980 Aug;77(8):5018–5020. doi: 10.1073/pnas.77.8.5018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lewis R. V., Stern A. S., Rossier J., Stein S., Udenfriend S. Putative enkephalin precursors in bovine adrenal medulla. Biochem Biophys Res Commun. 1979 Aug 13;89(3):822–829. doi: 10.1016/0006-291x(79)91852-7. [DOI] [PubMed] [Google Scholar]
  17. Noda M., Furutani Y., Takahashi H., Toyosato M., Hirose T., Inayama S., Nakanishi S., Numa S. Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin. Nature. 1982 Jan 21;295(5846):202–206. doi: 10.1038/295202a0. [DOI] [PubMed] [Google Scholar]
  18. Stein S., Böhlen P., Stone J., Dairman W., Udenfriend S. Amino acid analysis with fluorescamine at the picomole level. Arch Biochem Biophys. 1973 Mar;155(1):202–212. doi: 10.1016/s0003-9861(73)80022-0. [DOI] [PubMed] [Google Scholar]
  19. Stern A. S., Jones B. N., Shively J. E., Stein S., Undenfriend S. Two adrenal opioid polypeptides: proposed intermediates in the processing of proenkephalin. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1962–1966. doi: 10.1073/pnas.78.3.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stern A. S., Lewis R. V., Kimura S., Rossier J., Gerber L. D., Brink L., Stein S., Udenfriend S. Isolation of the opioid heptapeptide Met-enkephalin [Arg6,Phe7] from bovine adrenal medullary granules and striatum. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6680–6683. doi: 10.1073/pnas.76.12.6680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stern A. S., Lewis R. V., Kimura S., Rossier J., Stein S., Udenfriend S. Opioid hexapeptides and heptapeptides in adrenal medulla and brain possible implications on the biosynthesis of enkephalins. Arch Biochem Biophys. 1980 Dec;205(2):606–613. doi: 10.1016/0003-9861(80)90144-7. [DOI] [PubMed] [Google Scholar]

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