Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 May;79(10):3171–3175. doi: 10.1073/pnas.79.10.3171

Recombinational bypass of pyrimidine dimers promoted by the recA protein of Escherichia coli.

Z Livneh, I R Lehman
PMCID: PMC346376  PMID: 6954468

Abstract

recA protein, in the presence of single-stranded DNA binding protein and ATP, promotes the complete exchange of strands between circular single-stranded DNA containing pyrimidine dimers and a homologous linear duplex, converting the pyrimidine dimer-containing single-stranded DNA to a circular duplex. Bypass of a pyrimidine dimer during the branch-migration phase of the reaction requires approximately 20 seconds, a rate 1/50th of that in the absence of the dimer. The circular duplex product is specifically incised by the pyrimidine dimer-specific T4 endonuclease V, and the resulting 3' hydroxyl termini can serve as primers for deoxynucleotide polymerization by DNA polymerase I. These findings indicate that recA protein serves a direct role in recombinational repair and demonstrate that the pyrimidine dimers that have been bypassed can be processed by enzymes of the excision-repair pathway.

Full text

PDF
3171

Images in this article

3173Image
on p.3173

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Castellazzi M., Jacques M., George J. tif-Stimulated deoxyribonucleic acid repair in Escherichia coli K-12. J Bacteriol. 1980 Aug;143(2):703–709. doi: 10.1128/jb.143.2.703-709.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cox M. M., Lehman I. R. Directionality and polarity in recA protein-promoted branch migration. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6018–6022. doi: 10.1073/pnas.78.10.6018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cox M. M., Lehman I. R. recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3433–3437. doi: 10.1073/pnas.78.6.3433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cox M. M., McEntee K., Lehman I. R. A simple and rapid procedure for the large scale purification of the recA protein of Escherichia coli. J Biol Chem. 1981 May 10;256(9):4676–4678. [PubMed] [Google Scholar]
  5. DasGupta C., Shibata T., Cunningham R. P., Radding C. M. The topology of homologous pairing promoted by RecA protein. Cell. 1980 Nov;22(2 Pt 2):437–446. doi: 10.1016/0092-8674(80)90354-2. [DOI] [PubMed] [Google Scholar]
  6. Demple B., Linn S. DNA N-glycosylases and UV repair. Nature. 1980 Sep 18;287(5779):203–208. doi: 10.1038/287203a0. [DOI] [PubMed] [Google Scholar]
  7. Eisenberg S., Harbers B., Hours C., Denhardt D. T. The mechanism of replication of phiX174 DNA. XII. Non-random location of gaps in nascent phiX174 RF II DNA. J Mol Biol. 1975 Nov 25;99(1):107–123. doi: 10.1016/s0022-2836(75)80162-8. [DOI] [PubMed] [Google Scholar]
  8. Gottesman S. Genetic control of the SOS system in E. coli. Cell. 1981 Jan;23(1):1–2. doi: 10.1016/0092-8674(81)90261-0. [DOI] [PubMed] [Google Scholar]
  9. Hanawalt P. C., Cooper P. K., Ganesan A. K., Smith C. A. DNA repair in bacteria and mammalian cells. Annu Rev Biochem. 1979;48:783–836. doi: 10.1146/annurev.bi.48.070179.004031. [DOI] [PubMed] [Google Scholar]
  10. Iyer V. N., Rupp W. D. Usefulness of benzoylated naphthoylated DEAE-cellulose to distinguish and fractionate double-stranded DNA bearing different extents of single-stranded regions. Biochim Biophys Acta. 1971 Jan 1;228(1):117–126. doi: 10.1016/0005-2787(71)90551-x. [DOI] [PubMed] [Google Scholar]
  11. Moore P. D., Bose K. K., Rabkin S. D., Strauss B. S. Sites of termination of in vitro DNA synthesis on ultraviolet- and N-acetylaminofluorene-treated phi X174 templates by prokaryotic and eukaryotic DNA polymerases. Proc Natl Acad Sci U S A. 1981 Jan;78(1):110–114. doi: 10.1073/pnas.78.1.110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mosbaugh D. W., Linn S. Characterization of the action of Escherichia coli DNA polymerase I at incisions produced by repair endodeoxyribonucleases. J Biol Chem. 1982 Jan 10;257(1):575–583. [PubMed] [Google Scholar]
  13. Nakabeppu Y., Sekiguchi M. Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA. Proc Natl Acad Sci U S A. 1981 May;78(5):2742–2746. doi: 10.1073/pnas.78.5.2742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Radany E. H., Friedberg E. C. A pyrimidine dimer-DNA glycosylase activity associated with the v gene product of bacterophage T4. Nature. 1980 Jul 10;286(5769):182–185. doi: 10.1038/286182a0. [DOI] [PubMed] [Google Scholar]
  15. Radding C. M. Recombination activities of E. coli recA protein. Cell. 1981 Jul;25(1):3–4. doi: 10.1016/0092-8674(81)90224-5. [DOI] [PubMed] [Google Scholar]
  16. Razin A., Sedat J. W., Sinsheimer R. L. Structure of the DNA of bacteriophage phiX174. VII. Methylation. J Mol Biol. 1970 Oct 28;53(2):251–259. doi: 10.1016/0022-2836(70)90298-6. [DOI] [PubMed] [Google Scholar]
  17. Rupp W. D., Howard-Flanders P. Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J Mol Biol. 1968 Jan 28;31(2):291–304. doi: 10.1016/0022-2836(68)90445-2. [DOI] [PubMed] [Google Scholar]
  18. Rupp W. D., Wilde C. E., 3rd, Reno D. L., Howard-Flanders P. Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli. J Mol Biol. 1971 Oct 14;61(1):25–44. doi: 10.1016/0022-2836(71)90204-x. [DOI] [PubMed] [Google Scholar]
  19. Setlow R. B., Carrier W. L. Pyrimidine dimers in ultraviolet-irradiated DNA's. J Mol Biol. 1966 May;17(1):237–254. doi: 10.1016/s0022-2836(66)80105-5. [DOI] [PubMed] [Google Scholar]
  20. Warner H. R., Demple B. F., Deutsch W. A., Kane C. M., Linn S. Apurinic/apyrimidinic endonucleases in repair of pyrimidine dimers and other lesions in DNA. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4602–4606. doi: 10.1073/pnas.77.8.4602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weinstock G. M., McEntee K., Lehman I. R. ATP-dependent renaturation of DNA catalyzed by the recA protein of Escherichia coli. Proc Natl Acad Sci U S A. 1979 Jan;76(1):126–130. doi: 10.1073/pnas.76.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. West S. C., Cassuto E., Howard-Flanders P. Heteroduplex formation by recA protein: polarity of strand exchanges. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6149–6153. doi: 10.1073/pnas.78.10.6149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. West S. C., Cassuto E., Howard-Flanders P. Mechanism of E. coli RecA protein directed strand exchanges in post-replication repair of DNA. Nature. 1981 Dec 17;294(5842):659–662. doi: 10.1038/294659a0. [DOI] [PubMed] [Google Scholar]
  24. Witkin E. M. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev. 1976 Dec;40(4):869–907. doi: 10.1128/br.40.4.869-907.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES