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. 1982 Jun;79(11):3393–3397. doi: 10.1073/pnas.79.11.3393

Human papilloma viral DNA replicates as a stable episome in cultured epidermal keratinocytes.

R F LaPorta, L B Taichman
PMCID: PMC346426  PMID: 6285348

Abstract

Human papilloma virus (HPV) is poorly understood because systems for its growth in tissue culture have not been developed. We report here that cultured human epidermal keratinocytes could be infected with HPV from plantar warts and that the viral DNA persisted and replicated as a stable episome. There were 50-200 copies of viral DNA per cell and there was no evidence to indicate integration of viral DNA into the cellular genome. There was also no evidence to suggest that viral DNA underwent productive replication. We conclude that cultured human epidermal keratinocytes may be a model for the study of certain aspects of HPV biology.

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