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. 1982 Jun;79(11):3594–3598. doi: 10.1073/pnas.79.11.3594

Murine T cell-specific Ia antigens: monoclonal antibodies define an I-A-encoded T lymphocyte structure.

C E Hayes, D A Hullett
PMCID: PMC346469  PMID: 6980412

Abstract

T lymphocytes express a unique I-A subregion-controlled surface molecule that is not expressed on B lymphocytes. We produced antisera and monoclonal antibodies recognizing this structure. Exhaustive B cell adsorption (A.TH X B10.HTT)F antiserum, produced against activated A.TL T cells, left antibodies that bind an I-Ak specificity on some B10.A(4R) T lymphocytes (11 +/- 2%, mean +/- SEM). Similarly, exhaustive B cell adsorption of (A/J X B10.MBR)F antiserum, produced against activated B10.A(5R) cells, left antibodies specific for an I-Ab determinant on B10.A(5R)T cells (17 +/- 2%). We fused A.TL-immune (A.TH X B10.HTT)F splenocytes with NS-1 myeloma cells and identified antibody-producing hybrid cells by a fluorescent enzyme-linked immunosorbent microassay described herein. Eight monoclonal antibodies were selected; these lyse 7--26% of peripheral T cells from I-Ak strains. Thymocytes and bone marrow cells do not express the I-Ak T cells determinant. Exhaustive B cell adsorption did not remove I-Ak T cell-specific monoclonal antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benacerraf B., Germain R. N. The immune response genes of the major histocompatibility complex. Immunol Rev. 1978;38:70–119. doi: 10.1111/j.1600-065x.1978.tb00385.x. [DOI] [PubMed] [Google Scholar]
  2. David C. S. Serologic and genetic aspects of murine Ia antigens. Transplant Rev. 1976;30:299–302. doi: 10.1111/j.1600-065x.1976.tb00224.x. [DOI] [PubMed] [Google Scholar]
  3. David C., Meo T., McCormick J., Shreffler D. Expression of individual Ia specificities on T and B cells. I. Studies with mitogen-induced blast cells. J Exp Med. 1976 Jan 1;143(1):218–224. doi: 10.1084/jem.143.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Debré P., Kapp J. A., Dorf M. E., Benacerraf B. Genetic control of specific immune suppression. II. H-2-linked dominant genetic control of immune suppression by the random copolymer L-glutamic acid50-L-tyrosine50 (GT). J Exp Med. 1975 Dec 1;142(6):1447–1454. doi: 10.1084/jem.142.6.1447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Erb P., Feldmann M. The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens. J Exp Med. 1975 Aug 1;142(2):460–472. doi: 10.1084/jem.142.2.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fathman C. G., Cone J. L., Sharrow S. O., Tyrer H., Sachs D. H. Ia alloantigen(s) detected on thymocytes by use of a fluorescence-activated cell sorter. J Immunol. 1975 Aug;115(2):584–589. [PubMed] [Google Scholar]
  7. Fathman C. G., Kimoto M., Melvold R., David C. S. Reconstitution of Ir genes, Ia antigens, and mixed lymphocyte reaction determinants by gene complementation. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1853–1857. doi: 10.1073/pnas.78.3.1853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frelinger J. A., Neiderhuber J. E., David C. S., Shreffler D. C. Evidence for the expression of Ia (H-2-associated) antigens on thymus-derived lymphocytes. J Exp Med. 1974 Nov 1;140(5):1273–1284. doi: 10.1084/jem.140.5.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goding J. W., White E., Marchalonis J. J. Partial characterisation of Ia antigens on murine thymocytes. Nature. 1975 Sep 18;257(5523):230–231. doi: 10.1038/257230a0. [DOI] [PubMed] [Google Scholar]
  10. Hayes C. E., Bach F. H. I-N: a newly described H-2 I subregion between K and I-A. J Exp Med. 1980 Feb 1;151(2):481–485. doi: 10.1084/jem.151.2.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hayes C. E., Bach F. H. Murine T cell Ia antigens. II. Further studies on an I-J subregion specificity. J Immunol. 1979 Oct;123(4):1678–1686. [PubMed] [Google Scholar]
  12. Hayes C. E., Maier J. M. Murine T cell Ia antigens: specificity Iat.46 maps in I-J to I-E chromosomal segment of H-2 complex. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6783–6787. doi: 10.1073/pnas.77.11.6783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KAMINSKI M. Studies on egg white and its constituents by immunochemical techniques in gelified media: specific precipitation by double diffusion and immunoelectrophoretic analysis. J Immunol. 1955 Nov;75(5):367–376. [PubMed] [Google Scholar]
  14. Kanno M., Kobayashi S., Tokuhisa T., Takei I., Shinohara N., Taniguchi M. Monoclonal antibodies that recognize the product controlled by a gene in the I-J subregion of the mouse H-2 complex. J Exp Med. 1981 Nov 1;154(5):1290–1304. doi: 10.1084/jem.154.5.1290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Katz D. H., Graves M., Dorf M. E., Dimuzio H., Benacerraf B. Cell interactions between histoincompatible T and B lymphocytes. VII. Cooperative responses between lymphocytes are controlled by genes in the I region of the H-2 complex. J Exp Med. 1975 Jan 1;141(1):263–268. doi: 10.1084/jem.141.1.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  17. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  18. Lerner E. A., Matis L. A., Janeway C. A., Jr, Jones P. P., Schwartz R. H., Murphy D. B. Monoclonal antibody against an Ir gene product? J Exp Med. 1980 Oct 1;152(4):1085–1101. doi: 10.1084/jem.152.4.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. McDevitt H. O., Deak B. D., Shreffler D. C., Klein J., Stimpfling J. H., Snell G. D. Genetic control of the immune response. Mapping of the Ir-1 locus. J Exp Med. 1972 Jun 1;135(6):1259–1278. doi: 10.1084/jem.135.6.1259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Murphy D. B., Herzenberg L. A., Okumura K., Herzenberg L. A., McDevitt H. O. A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes. J Exp Med. 1976 Sep 1;144(3):699–712. doi: 10.1084/jem.144.3.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Murphy D. B., Shrefler D. C. Cross-reactivity between H-2K and H-2D products. I. Evidence for extensive and reciprocal serological cross-reactivity. J Exp Med. 1975 Feb 1;141(2):374–391. doi: 10.1084/jem.141.2.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Okumura K., Herzenberg L. A., Murphy D. B., McDevitt H. O., Herzenberg L. A. Selective expression of H-2 (i-region) loci controlling determinants on helper and suppressor T lymphocytes. J Exp Med. 1976 Sep 1;144(3):685–698. doi: 10.1084/jem.144.3.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ozato K., Mayer N., Sachs D. H. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980 Feb;124(2):533–540. [PubMed] [Google Scholar]
  25. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sachs D. H. The Ia antigens. Contemp Top Mol Immunol. 1976;5:1–33. doi: 10.1007/978-1-4684-8142-6_1. [DOI] [PubMed] [Google Scholar]
  27. Schwartz R. H., David C. S., Sachs D. H., Paul W. E. T lymphocyte-enriched murine peritoneal exudate cells. III. Inhibition of antigen-induced T lymphocyte Proliferation with anti-Ia antisera. J Immunol. 1976 Aug;117(2):531–540. [PubMed] [Google Scholar]
  28. Shreffler D. C., David C. S. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975;20:125–195. doi: 10.1016/s0065-2776(08)60208-4. [DOI] [PubMed] [Google Scholar]
  29. Swierkosz J. E., Marrack P., Kappler J. W. Functional analysis of T cells expressing Ia antigens. I. Demonstration of helper T-cell heterogeneity. J Exp Med. 1979 Dec 1;150(6):1293–1309. doi: 10.1084/jem.150.6.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tada T., Okumura K. The role of antigen-specific T cell factors in the immune response. Adv Immunol. 1979;28:1–87. doi: 10.1016/s0065-2776(08)60799-3. [DOI] [PubMed] [Google Scholar]
  31. Tokuhisa T., Taniguchi M., Okumura K., Tada T. An antigen-specific I region gene product that augments the antibody response. J Immunol. 1978 Feb;120(2):414–421. [PubMed] [Google Scholar]
  32. Uhr J. W., Capra J. D., Vitetta E. S., Cook R. G. Organization of the immune response genes. Science. 1979 Oct 19;206(4416):292–297. doi: 10.1126/science.113876. [DOI] [PubMed] [Google Scholar]
  33. Waltenbaugh C. Regulation of immune responses by I-J gene products. I. Production and characterization of anti-I-J monoclonal antibodies. J Exp Med. 1981 Nov 1;154(5):1570–1583. doi: 10.1084/jem.154.5.1570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Zinkernagel R. M., Callahan G. N., Althage A., Cooper S., Streilein J. W., Klein J. The lymphoreticular system in triggering virus plus self-specific cytotoxic T cells: evidence for T help. J Exp Med. 1978 Mar 1;147(3):897–911. doi: 10.1084/jem.147.3.897. [DOI] [PMC free article] [PubMed] [Google Scholar]

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