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. 1982 Jul;79(13):4143–4147. doi: 10.1073/pnas.79.13.4143

Insertion of a movable genetic element, 297, into the T-A-T-A box for the H3 histone gene in Drosophila melanogaster.

H Ikenaga, K Saigo
PMCID: PMC346593  PMID: 6287469

Abstract

From a cloned library of Drosophila DNA, a recombinant phage was isolated which contained a nucleotide sequence homologous to both the histone gene and a movable genetic element, 297 [Saigo, K., Millstein, L. & Thomas, C.A., Jr. (1981) Cold Spring Harbor Symp. Quant. Biol. 45, 815-827]. We have determined the nucleotide sequences of the termini of 297 as well as its insertion site in the histone gene in the clone. Our results strongly suggest that (i) although 297 ends with typical long terminal repeats, integrated 297 is not bounded by 5' T-G...C-A 3', dinucleotides commonly found at the very ends of integrated forms of other similar genetic elements, and (ii) the insertion of 297 occurred exactly within the T-A-T-A box for the H3 histone gene containing T-A-T-A-T-A. The structures of the insertion sites of 297 were also examined by using two other recombinant clones of the histone gene. A 6-base-pair-long segment, T-A-T-A-T-A, was again found to be present at the insertion sites of 297 in both clones. Based on these results, it was concluded that, at least within the histone gene cluster, the insertion of 297 was carried out in a site-specific fashion with T-A-T-A-T-A as the target sequence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Breathnach R., Chambon P. Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981;50:349–383. doi: 10.1146/annurev.bi.50.070181.002025. [DOI] [PubMed] [Google Scholar]
  3. Cameron J. R., Loh E. Y., Davis R. W. Evidence for transposition of dispersed repetitive DNA families in yeast. Cell. 1979 Apr;16(4):739–751. doi: 10.1016/0092-8674(79)90090-4. [DOI] [PubMed] [Google Scholar]
  4. Fink G., Farabaugh P., Roeder G., Chaleff D. Transposable elements (Ty) in yeast. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):575–580. doi: 10.1101/sqb.1981.045.01.074. [DOI] [PubMed] [Google Scholar]
  5. Finnegan D. J., Rubin G. M., Young M. W., Hogness D. S. Repeated gene families in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1053–1063. doi: 10.1101/sqb.1978.042.01.106. [DOI] [PubMed] [Google Scholar]
  6. Flavell A. J., Ish-Horowicz D. Extrachromosomal circular copies of the eukaryotic transposable element copia in cultured Drosophila cells. Nature. 1981 Aug 13;292(5824):591–595. doi: 10.1038/292591a0. [DOI] [PubMed] [Google Scholar]
  7. Greer H., Igo M., de Bruijn F. Transposable elements involving the his4 region of yeast. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):567–574. doi: 10.1101/sqb.1981.045.01.073. [DOI] [PubMed] [Google Scholar]
  8. Grosschedl R., Birnstiel M. L. Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1432–1436. doi: 10.1073/pnas.77.3.1432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hovemann B., Galler R., Walldorf U., Küpper H., Bautz E. K. Vitellogenin in Drosophila melanogaster: sequence of the yolk protein I gene and its flanking regions. Nucleic Acids Res. 1981 Sep 25;9(18):4721–4734. doi: 10.1093/nar/9.18.4721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ilyin Y. V., Tchurikov N. A., Ananiev E. V., Ryskov A. P., Yenikolopov G. N., Limborska S. A., Maleeva N. E., Gvozdev V. A., Georgiev G. P. Studies on the DNA fragments of mammals and Drosophila containing structural genes and adjacent sequences. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):959–969. doi: 10.1101/sqb.1978.042.01.097. [DOI] [PubMed] [Google Scholar]
  11. Lifton R. P., Goldberg M. L., Karp R. W., Hogness D. S. The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1047–1051. doi: 10.1101/sqb.1978.042.01.105. [DOI] [PubMed] [Google Scholar]
  12. Majors J. E., Swanstrom R., DeLorbe W. J., Payne G. S., Hughes S. H., Ortiz S., Quintrell N., Bishop J. M., Varmus H. E. DNA intermediates in the replication of retroviruses are structurally (and perhaps functionally) related to transposable elements. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):731–738. doi: 10.1101/sqb.1981.045.01.091. [DOI] [PubMed] [Google Scholar]
  13. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  14. Neel B. G., Hayward W. S., Robinson H. L., Fang J., Astrin S. M. Avian leukosis virus-induced tumors have common proviral integration sites and synthesize discrete new RNAs: oncogenesis by promoter insertion. Cell. 1981 Feb;23(2):323–334. doi: 10.1016/0092-8674(81)90128-8. [DOI] [PubMed] [Google Scholar]
  15. Norgard M. V. Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase. Anal Biochem. 1981 May 1;113(1):34–42. doi: 10.1016/0003-2697(81)90040-3. [DOI] [PubMed] [Google Scholar]
  16. Ozaki L. S., Maeda S., Shimada K., Takagi Y. A novel ColE1::Tn3 plasmid vector that allows direct selection of hybrid clones in E. coli. Gene. 1980 Feb;8(3):301–314. doi: 10.1016/0378-1119(80)90006-2. [DOI] [PubMed] [Google Scholar]
  17. Potter S. S., Brorein W. J., Jr, Dunsmuir P., Rubin G. M. Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell. 1979 Jun;17(2):415–427. doi: 10.1016/0092-8674(79)90168-5. [DOI] [PubMed] [Google Scholar]
  18. Rubin G. M., Brorein W. J., Jr, Dunsmuir P., Flavell A. J., Levis R., Strobel E., Toole J. J., Young E. Copia-like transposable elements in the Drosophila genome. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):619–628. doi: 10.1101/sqb.1981.045.01.080. [DOI] [PubMed] [Google Scholar]
  19. Saigo K., Millstein L., Thomas C. A., Jr The organization of Drosophila melanogaster histone genes. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):815–827. doi: 10.1101/sqb.1981.045.01.100. [DOI] [PubMed] [Google Scholar]
  20. Shimotohno K., Temin H. M. Evolution of retroviruses from cellular movable genetic elements. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):719–730. doi: 10.1101/sqb.1981.045.01.090. [DOI] [PubMed] [Google Scholar]
  21. Shoemaker C., Goff S., Gilboa E., Paskind M., Mitra S. W., Baltimore D. Structure of cloned retroviral circular DNAs: implications for virus integration. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):711–717. doi: 10.1101/sqb.1981.045.01.089. [DOI] [PubMed] [Google Scholar]
  22. Strobel E., Dunsmuir P., Rubin G. M. Polymorphisms in the chromosomal locations of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell. 1979 Jun;17(2):429–439. doi: 10.1016/0092-8674(79)90169-7. [DOI] [PubMed] [Google Scholar]
  23. Sutcliffe J. G., Shinnick T. M., Lerner R. A. Moloney murine leukemia virus is a transposon: nucleotide sequence analysis identifies genes and replication details. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):707–710. doi: 10.1101/sqb.1981.045.01.088. [DOI] [PubMed] [Google Scholar]
  24. Temin H. M. Structure, variation and synthesis of retrovirus long terminal repeat. Cell. 1981 Nov;27(1 Pt 2):1–3. doi: 10.1016/0092-8674(81)90353-6. [DOI] [PubMed] [Google Scholar]
  25. Williamson V. M., Young E. T., Ciriacy M. Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II. Cell. 1981 Feb;23(2):605–614. doi: 10.1016/0092-8674(81)90156-2. [DOI] [PubMed] [Google Scholar]

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