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. Author manuscript; available in PMC: 2012 Oct 8.
Published in final edited form as: Cytokine. 2011 Aug 30;56(2):325–331. doi: 10.1016/j.cyto.2011.08.018

Figure 3.

Figure 3

Cytokine transcript expression in PBMCs stimulated with ESAT-6 and CFP-10 peptides (10 µg/ml) for 48 hours, as indicated in Materials and Methods. Groups are the same as those described under Figure 1. Total RNA was isolated from PBMC following culture and cytokine expression was estimated by quantitative real-time PCR. Relative expression was calculated based on ΔCt values and expressed as Arbitrary Units, calculated by multiplying the ratio of the expression of each cytokine transcript to that of β-actin by 106. The three graphs (A-C) group different cytokines according to expression levels (A: < 100; B: 100–1,500; C: >1,500 Arbitrary Units). Bars represent the geometric mean of each group (in Arbitrary Units) and error bars represent 95% C.I. (* p<0.05; ** p<0.01).