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. 1982 Jul;79(14):4256–4259. doi: 10.1073/pnas.79.14.4256

Expression of a chemically synthesized human alpha 1 interferon gene.

E De Maeyer, D Skup, K S Prasad, J De Maeyer-Guignard, B Williams, P Meacock, G Sharpe, D Pioli, J Hennam, W Schuch, K Atherton
PMCID: PMC346649  PMID: 6181502

Abstract

Cells of Escherichia coli containing a chemically synthesized human alpha 1 interferon (IFN-alpha 1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-alpha 1 [antiviral activity, acid stability, species crossreactivity, inactivation by antisera directed against leukocyte or Namalwa cell interferon, and stimulation of (2'-5')oligoadenylate synthetase activity]. Similar levels of IFN synthesis were obtained when the expression unit (lac promoter plus synthetic IFN-alpha 1 gene) was transplanted into the obligate methylotroph Methylophilus methylotrophus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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