Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Jul;79(14):4280–4284. doi: 10.1073/pnas.79.14.4280

alpha-Actinin interacts specifically with model membranes containing glycerides and fatty acids.

R K Meyer, H Schindler, M M Burger
PMCID: PMC346654  PMID: 6956857

Abstract

A method was developed to identify specific protein-lipid interactions of complex lipid mixtures and to assess their effect upon the arrangement of such complexes in monolayers at an air-water interface. Its application to striated muscle alpha-actinin revealed that just two lipids selectively interact with alpha-actinin. One molecule of glyceride and one molecule of fatty acid were found to be associated in a constant stoichiometry with one molecule of the alpha-actinin dimer. In the presence of both glycerides and fatty acids unexpectedly rigid monolayer areas formed. This lipid specificity could be confirmed by brief protease digestion of alpha-actinin liposome mixtures followed by peptide analysis; the peptide patterns of alpha-actinin depended on the presence or absence of only these two lipids. Possible implications of these findings are discussed in the context of Z-line formation.

Full text

PDF
4280

Images in this article

4282Image
on p.4282

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Bretscher A., Vandekerckhove J., Weber K. alpha-Actinins from chicken skeletal muscle and smooth muscle show considerable chemical and immunological differences. Eur J Biochem. 1979 Oct;100(1):237–243. doi: 10.1111/j.1432-1033.1979.tb02054.x. [DOI] [PubMed] [Google Scholar]
  3. Burridge K., McCullough L. The association of alpha-actinin with the plasma membrane. J Supramol Struct. 1980;13(1):53–65. doi: 10.1002/jss.400130106. [DOI] [PubMed] [Google Scholar]
  4. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  5. Geiger B., Singer S. J. The participation of alpha-actinin in the capping of cell membrane components. Cell. 1979 Jan;16(1):213–222. doi: 10.1016/0092-8674(79)90202-2. [DOI] [PubMed] [Google Scholar]
  6. Geiger B., Tokuyasu K. T., Dutton A. H., Singer S. J. Vinculin, an intracellular protein localized at specialized sites where microfilament bundles terminate at cell membranes. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4127–4131. doi: 10.1073/pnas.77.7.4127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jockusch B. M., Burger M. M., DaPrada M., Richards J. G., Chaponnier C., Gabbiani G. alpha-Actinin attached to membranes of secretory vesicles. Nature. 1977 Dec 15;270(5638):628–629. doi: 10.1038/270628a0. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lane B. P., Elias J., Drummond E. Immunoelectronmicroscopic localization of alpha-actinin in skeletal muscle cells. J Histochem Cytochem. 1977 Jan;25(1):69–72. doi: 10.1177/25.1.833423. [DOI] [PubMed] [Google Scholar]
  10. Lazarides E., Granger B. L. Fluorescent localization of membrane sites in glycerinated chicken skeletal muscle fibers and the relationship of these sites to the protein composition of the Z disc. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3683–3687. doi: 10.1073/pnas.75.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Loor F. Cell surface design. Nature. 1976 Nov 18;264(5583):272–273. doi: 10.1038/264272a0. [DOI] [PubMed] [Google Scholar]
  12. Omary M. B., Trowbridge I. S. Covalent binding of fatty acid to the transferrin receptor in cultured human cells. J Biol Chem. 1981 May 25;256(10):4715–4718. [PubMed] [Google Scholar]
  13. Osborn M., Weber K. Microfilament-associated proteins in tissue culture cells viewed by stereo immunofluorescence microscopy. Eur J Cell Biol. 1979 Oct;20(1):28–36. [PubMed] [Google Scholar]
  14. Papahadjopoulos D., Poste G., Schaeffer B. E. Fusion of mammalian cells by unilamellar lipid vesicles: inflluence of lipid surface charge, fluidity and cholesterol. Biochim Biophys Acta. 1973 Sep 27;323(1):23–42. doi: 10.1016/0005-2736(73)90429-x. [DOI] [PubMed] [Google Scholar]
  15. Podlubnaya Z. A., Tskhovrebova L. A., Zaalishtsbvili M. M., Stefanenko G. A. Electron microscopic study of alpha-actinin. J Mol Biol. 1975 Feb 25;92(2):357–359. doi: 10.1016/0022-2836(75)90234-x. [DOI] [PubMed] [Google Scholar]
  16. Robson R. M., Goll D. E., Arakawa N., Stromer M. H. Purification and properties of alpha-actinin from rabbit skeletal muscle. Biochim Biophys Acta. 1970 Feb 17;200(2):296–318. doi: 10.1016/0005-2795(70)90173-x. [DOI] [PubMed] [Google Scholar]
  17. Schlesinger M. J., Magee A. I., Schmidt M. F. Fatty acid acylation of proteins in cultured cells. J Biol Chem. 1980 Nov 10;255(21):10021–10024. [PubMed] [Google Scholar]
  18. Schmidt M. F., Schlesinger M. J. Fatty acid binding to vesicular stomatitis virus glycoprotein: a new type of post-translational modification of the viral glycoprotein. Cell. 1979 Aug;17(4):813–819. doi: 10.1016/0092-8674(79)90321-0. [DOI] [PubMed] [Google Scholar]
  19. Stromer M. H., Goll D. E. Studies on purified -actinin. II. Electron microscopic studies on the competitive binding of -actinin and tropomyosin to Z-line extracted myofibrils. J Mol Biol. 1972 Jun 28;67(3):489–494. doi: 10.1016/0022-2836(72)90465-2. [DOI] [PubMed] [Google Scholar]
  20. Suzuki A., Goll D. E., Singh I., Allen R. E., Robson R. M., Stromer M. H. Some properties of purified skeletal muscle alpha-actinin. J Biol Chem. 1976 Nov 10;251(21):6860–6870. [PubMed] [Google Scholar]
  21. Wehland J., Osborn M., Weber K. Cell-to-substratum contacts in living cells: a direct correlation between interference-reflexion and indirect-immunofluorescence microscopy using antibodies against actin and alpha-actinin. J Cell Sci. 1979 Jun;37:257–273. doi: 10.1242/jcs.37.1.257. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES