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. 1997 Nov;25(1-3):205–211. doi: 10.1023/A:1007947329496

Immunomodulating activities of polysaccharide fractions from dried safflower petals

Tetsuya Wakabayashi, Shigenari Hirokawa, Noriaki Yamauchi, Takao Kataoka, Je-Tae Woo, Kazuo Nagai
PMCID: PMC3466740  PMID: 22358893

Abstract

In the course of screening for immunomodulators, we found a significant blastogenic activity specific for splenic B cells in the extracts of safflower (Carthamus tinctorius L.). Active fractions termed SF1 and SF2 were purified from dried petals of safflower by boiling water extraction, ethanol precipitation and Sepharose CL-2B column chromatography. The elution profiles of the gel filtration indicated that the molecular weight of SF1 and SF2 was estimated to be more than 100 kD. Major components of SF1 and SF2 seem to be polysaccharides, and structural analysis of alditol acetate derivatives by GC-MS revealed some differences between SF1 and SF2 in the sugar component. Biological activities of SF1 and SF2 on B cells and macrophages were examined in comparison with lipopolysaccharides (LPS). SF1 and SF2 induced both the proliferation and the IgM production of B cells to the equivalent level as those induced by LPS. In macrophages, SF1 and SF2 effectively stimulated the production of NO. However, SF1 stimulated the production of IL-1, IL-6, and TNF as much as LPS, while SF2 induced them only weakly or not at all. Thus, these results suggest that SF1 and SF2 activate B cells and macrophages in different mechanisms.

Keywords: B cell, Carthamus tinctorius L., cytokine, lipopolysaccharides, macrophages, polysaccharide, safflower

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References

  1. Brander WT, Clarke DA, Stock CC. Stimulation of Host Defence against Experimental Cancer I. Zymosan and Sarcoma 180 in mice. Cancer Res. 1958;18:347–351. [PubMed] [Google Scholar]
  2. Brander WT, Clarke DA. Stimulation of Host Defence against Experimental Cancer II. Temporal and Reversal Studies of the Zymosan Effect. Cancer Res. 1959;19:673–678. [PubMed] [Google Scholar]
  3. Chihara G, Maeda Y, Hamuro J, Sasaki T, Fukuoka F. Inhibition of Mouse Sarcoma 180 by Polysaccharides from Lentinus edodes (Berk.) Sing. Nature. 1969;222:687–688. doi: 10.1038/222687a0. [DOI] [PubMed] [Google Scholar]
  4. Chihara G, Hamoro J, Maeda YY, Arai Y, Fukuoka F. Fractionation and Purification of the Polysaccharides with Marked Antitumor Activity, Especally Lentinan, from Lentinus edodes (Berk.) Sing. (an Edible Mushroom) Cancer Res. 1970;30:2776–2781. [PubMed] [Google Scholar]
  5. Cohen L, Haziot A, Shen DR, Lin X, Sia C, Harper R, Silver J, Goyert SM. CD14-Independent Responses to LPS Require a Serum Factor That Is Absent from Neonates. J Immunol. 1995;155:5337–5342. [PubMed] [Google Scholar]
  6. Haziot A, Ferreo E, Köntgen F, Hijima N, Yamamoto S, Silver J, Stewart CL, Goyert SM. Resistance to Endotokin Shock and Reduced Dissemination of Gram-Negative Bacteria in CD14-Deficient Mice. Immunity. 1996;4:407–414. doi: 10.1016/S1074-7613(00)80254-X. [DOI] [PubMed] [Google Scholar]
  7. Hofstad T, Skaug N, Sveen K. Stimulation of B Lymphocytes by Lipopolusaccharides from Anaerobic Bacteria. Clin Infect Dis. 1993;16:200–202. doi: 10.1093/clinids/16.supplement_4.s200. [DOI] [PubMed] [Google Scholar]
  8. Itoh W, Sugawara I, Kimura S, Tabata K, Hirata A, Kojima T, Mori S, Shimada K. Immunopharmacological study of schizophyllan (SPG). I. — Its action as a mitogen and anti-HIV agent. Int J Immunopharmacol. 1990;12:225–233. doi: 10.1016/0192-0561(90)90057-T. [DOI] [PubMed] [Google Scholar]
  9. Kalechman Y, Albeck M, Oron M, Sobelman D, Gurwith M, Seghal S, Sredni B. Radioprotective effects of the immunomodulator AS101. J Immunol. 1990;145:1512–1517. [PubMed] [Google Scholar]
  10. Kalechman Y, Shani A, Dovrat S, Whisnant JK, Mettinger K, Albeck M, Sredni B. The Antitumoral Effect of the Immunomodulator AS101 and Paclitaxel (Taxol) in Murine Model of Lung Adenocarcinoma. J Immunol. 1996;156:1101–1109. [PubMed] [Google Scholar]
  11. Kato M, Hirose K, Hakozaki M, Ohno M, Saito Y, Izutani R, Noguchi J, Hori Y, Okumoto S, Kuroda D. Induction of gene expression for immunomodulating cytokines in peripheral blood mononuclear cells in response to orally administered PSK, an immunomodulating protein-bound polysaccharide. Cancer Immunol Immunother. 1995;40:152–156. doi: 10.1007/BF01517346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kawaguchi T, Suematsu M, Koizumi HM, Mitsui H, Suzuki S, Matsuno T, Ogawa H, Nomoto K. Activation of Macrophage Function by Intraperitoneal Administration of the Streptococcal Antitumor Agent OK-432. Immunopharmacol. 1983;6:177–189. doi: 10.1016/0162-3109(83)90019-X. [DOI] [PubMed] [Google Scholar]
  13. Kurakata Y, Sakagami H, Takeda M, Konno K, Kitajima K, Ichikawa S, Hara N, Sato T. Mitogenic activity of pine cone extracts against cultured splenocytes from normal and tumorbearing animals. Anticancer Res. 1989;9:961–966. [PubMed] [Google Scholar]
  14. Lim BO, Yamada K, Yoshimura K, Watanabe T, Hung P, Taniguchi S, Sugano M. Free Bile Acids Inhibit IgE Production by Mouse Spleen Lymphocytes Stimulated by Lipopolysaccharide and Interleukins. Biosci Biotech Biochem. 1995;59:624–627. doi: 10.1271/bbb.59.624. [DOI] [PubMed] [Google Scholar]
  15. Matsuda T, Hirano T, Kishimoto T. Establishment of an interleukin 6 (IL-6)/B cell stimulatory factor 2-dependent cell line and preparation of anti-IL-6 monoclonal antibodies. Eur J Immunol. 1988;18:951–956. doi: 10.1002/eji.1830180618. [DOI] [PubMed] [Google Scholar]
  16. Mizuno T, Kinoshita T, Zhuang C, Ito H, Mayuzumi Y. Antitumor-active heteroglycans from Niohshimeji mushroom, Tricholoma giganteum. Biosci Biotech Biochem. 1995;59:568–571. doi: 10.1271/bbb.59.568. [DOI] [PubMed] [Google Scholar]
  17. Nemoto J, Ohno N, Saito K, Adachi Y, Yadomae T. Analysis of Cytokine mRNAs Induced by the Administration of a Highly Branched (1→3)-beta-D-blucan, OL-2. Biol Pharm Bull. 1994;17:948–954. doi: 10.1248/bpb.17.948. [DOI] [PubMed] [Google Scholar]
  18. Nick JA, Avdi NJ, Gerwins P, Johnson GL, Worthen GS. Activation of a p38 Mitogen-Activated Protein Kinase in Human Neutrophils by Lipopolysaccharide. J Immunol. 1996;156:4867–4875. [PubMed] [Google Scholar]
  19. Sakagami H, Sugaya K, Utsumi A, Fujinaga S, Sato T, Takeda M. Stimulation by PSK of Interleukin-1 Production by Human Peripheral Blood Mononuclear Cells. Anticancer Res. 1993;13:671–676. [PubMed] [Google Scholar]
  20. Stimpel M, Proksch A, Wagner H, Lohmann-Matthes ML. Macrophage Activation and Induction of Macrophage Cytotoxicity by Purified Polysaccharide Fractions from the plant Echinacea purpurea. Infect Immun. 1984;46:845–849. doi: 10.1128/iai.46.3.845-849.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Takasuka N, Tokunaga T, Akagawa K. Preexposure of macrophages to low doses of lipopolysaccharide inhibits the expression of tumor necrosis factor-α mRNA but not of IL-1β mRNA. J Immunol. 1991;146:3824–3830. [PubMed] [Google Scholar]
  22. Wang G, Zhang J, Mizuno T, Zhuang C, Ito H, Mayuzumi H, Okamoto H, Li J. Antitumor Active Polysaccharides from the Chinese Mushroom Songshan Lingzhi, the Fruiting Body of Ganoderma tsugae. Biosci Biotech Biochem. 1993;57:894–900. doi: 10.1271/bbb.57.894. [DOI] [PubMed] [Google Scholar]
  23. Ziegler-Heitbrock HWL, Ulevitch RJ. CD14: Cell surface receptor and differentiation marker. Immunol Today. 1993;14:121–125. doi: 10.1016/0167-5699(93)90212-4. [DOI] [PubMed] [Google Scholar]

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