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. 1997 Nov;25(1-3):191–195. doi: 10.1023/A:1007982909932

Mannosylerythritol lipid induces granulocytic differentiation and inhibits the tyrosine phosphorylation of human myelogenous leukemia cell line K562

Hiroko Isoda, Tadaatsu Nakahara
PMCID: PMC3466747  PMID: 22358891

Abstract

Mannosylerythritol lipid (MEL), which induced granulocytic differentiation of human promyelocytic leukemia cell line HL60, also induced differentiation of human myelogenous leukemia cell line K562. MEL inhibited insulin-dependent cell proliferation and induced leukocyte esterase activity of K562 cells. MEL markedly increased the differentiation-associated characteristics in granulocytes, such as nitroblue tetrazolium (NBT) reducing ability, expression of Fc receptors, and phagocytic activity of K562 cells. The tyrosine phosphorylation in K562 cells inhibited by MEL. These results suggest that MEL directly down-regulates the tyrosine kinase activities in K562 cells to inhibit the cell proliferation and to induce the differentiation.

Keywords: differentiation, insulin-dependent, K562, phospho-tyrosine

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References

  1. Anderson LC, Nilsson K, Cahmberg CG. K562-A human erythroleukemic cell line Int. J. Cancer. 1979;23:143–147. doi: 10.1002/ijc.2910230202. [DOI] [PubMed] [Google Scholar]
  2. Bremer EG, hakomori S, Bowen-Pope DE, Raunes E, Ross R. Ganglioside-Mediated Modulation of Cell Growth, Growth Factor Binding and Receptor Phosphorylation. J. Biol. Chem. 1984;259:6818–6825. [PubMed] [Google Scholar]
  3. Bremer EG, Schlessinger J, Hakomori S. Ganglioside-mediated Modulation of Cell growht. J. Biol. Chem. 1986;261:2434–2440. [PubMed] [Google Scholar]
  4. Brietman TR, Selonick SE, Collins SJ. Induction of differentiation of the human promyelicytic cell line (HL60) by retinoic acid. Proc. Natl. Acad. Sci. USA. 1989;77:2936–2940. doi: 10.1073/pnas.77.5.2936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brietman TR, Keene BR, Henmi H. Cancer Survay. 1983;2:263–268. [Google Scholar]
  6. Collins SJ, Gallo RC, Gallagher RE. Continuous growth and differentiation of human myeloid leukemia cells in suspension culture. Nature. 1977;270:347–349. doi: 10.1038/270347a0. [DOI] [PubMed] [Google Scholar]
  7. Hakomori S and Igarashi Y (1992) Gangliosides and Glycosphingolipids as Modulators of Cell Growth, Adhesion, and Transmembrane Signaling, in Advances Lipid Research, Vol. 25, pp. 147–162, Accademic Press, Inc. [PubMed]
  8. Hanai N, Nores GA, MacLeod C, Rorrea-Mendez CR, Hakomori S. Ganglioside-mediated Modulation of Cell Growth. J. Biol. Chem. 1988;263:10915–10921. [PubMed] [Google Scholar]
  9. Hanai N, Dohi T, Nores GA, Hakomori S. A NOvel Ganglioside, De-N-acetyl HM3 (II3NeuHH2LacCer), Acting as a Strong Promoter for Epidermal Growth factor receptor Kinase and as a Stimulator for Cell Growth. J. Biol. Chem. 1988;263:6296–6301.1. [PubMed] [Google Scholar]
  10. Hozumi M. Fundamentals of chemotherapy of myeloid leukemia by induction of leukemia cell differentiation. Adv. Cancer Res. 1983;38:121–169. doi: 10.1016/S0065-230X(08)60189-X. [DOI] [PubMed] [Google Scholar]
  11. Igarashi Y, Nojiri H, Hanai N, Hakomori S. In: Gangliosides that modulate membrane protein function in Methods in Enzymology. Ginsburg V., editor. Orlando, FL: Academic Press; 1989. pp. 521–541. [DOI] [PubMed] [Google Scholar]
  12. Isoda H, Shinmoto H, Kitamoto D, Matsumura M, Makahara T. Differentiation of Human Prommyelocytic Leukemia Cell Line HL60 by Microbial Ectracellular Glycolipids. Lipids. 1995;32:263–271. doi: 10.1007/s11745-997-0033-0. [DOI] [PubMed] [Google Scholar]
  13. Kitamoto D, Haneishi K, Nakahara T, Tabuchi T. Production of mannosylerythritol lipids by (Candida antarctica from vegitable oils. Agric. Biol. Chem. 1990;54:37–40. [Google Scholar]
  14. Kitamoto D, Yanagishita H, Shinbo T, Nakane T, Kamisawa C, Nakahara T. Surface active properties and antimicrobial activities of mannosylerythritol lipids as biosurfactants produced by Candida antarctica. J. Biotech. 1993;29:91–96. doi: 10.1016/0168-1656(93)90042-L. [DOI] [Google Scholar]
  15. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680–684. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Li CY, Lam KW, Yam LT. Esterase in human leukocytes. J. Histochem. Cytochem. 1973;21:1–12. doi: 10.1177/21.1.1. [DOI] [PubMed] [Google Scholar]
  17. Lozzio CB, Lozzio BB. Human chronic myulogenous leukemia cell line with positive Philadelphia chromosome. Blood. 1975;45:321–334. [PubMed] [Google Scholar]
  18. McCarthy DM, San Miguel F, Freake HC, Green PM, Zola H, Catovsky D, Goldman JM. 1,25-Dihydroxyvitamin D3 inhibits proleferation of human promyelocytic leukemia (HL60) cells and induces monocyte-macrophage differentiation in HL60 and norma human bone marrow cells. Leuk. Res. 1983;7:51–55. doi: 10.1016/0145-2126(83)90057-7. [DOI] [PubMed] [Google Scholar]
  19. Nojiri H, Stroud M, Hakomori S. A specific Type of GAnglioside as a Modulator of Insulin-dependent Cell Growth and Insulin Receptor Tyrosine Kinase Activity. J. Biol. Chem. 1991;266:4531–4537. [PubMed] [Google Scholar]
  20. Rovera G, Santoli D, Damsky C. Human promyelocytic leukemia cells in culture differentiate into macrophage-like cells when treated with a phorbol diester. Proc. Natl. Acad. Sci. USA. 1979;76:2779–2783. doi: 10.1073/pnas.76.6.2779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rowley PT, Ohlsson-Wilhelm BM, Farley BA, LaBella S. Inducers ofythroid differentiation ion K562 human leukemia cells. Exp. Jematol. 1981;9:32–37. [PubMed] [Google Scholar]
  22. Sinclair J, MacClain D, Taetle R. Effect of Insulin-Like Growth Factor I on Growth of Human Leukemia Cells in Serumfree and Protein-Free Medium. Blood. 1988;72:66–72. [PubMed] [Google Scholar]
  23. Takeda K, Hosoi T, Noda M, Arimura H, Konno K. Effect of fibroblast-derived differentiation inducing factor on the differentiation of human monocytoid and myeloid leukemia cell lines. Biochem. Biophisic. Res. Com. 1988;155:24–31. doi: 10.1016/S0006-291X(88)81044-1. [DOI] [PubMed] [Google Scholar]
  24. Takeda K, Minowada J, Bloch A. Kinetics of appearance of differentiation-associated characteristics in ML-1, a line of human myeloblastic leukemia cells, after treatment with 12-O-tetradecanoylphorbol-13-acetate, dimenthyl sulfoxide, or 1-b-D-arabinofuranosylcytosine. Cancer Research. 1982;42:5152–5158. [PubMed] [Google Scholar]
  25. Zeng G, Ariga T, Gu XB, Yu RK. Regulation of glycolipid synthesis in HL-60 cells by antisense oligodeoxynucleotides to glucosyltransferase sequences: Effect on cellulat differentiation. Proc. Natl. Acad. Sci. USA. 1995;92:8670–8674. doi: 10.1073/pnas.92.19.8670. [DOI] [PMC free article] [PubMed] [Google Scholar]

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