Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Jul;79(14):4386–4390. doi: 10.1073/pnas.79.14.4386

Serological analysis of cell surface antigens of null cell acute lymphocytic leukemia by mouse monoclonal antibodies.

R Ueda, M Tanimoto, T Takahashi, S Ogata, K Nishida, R Namikawa, Y Nishizuka, K Ota
PMCID: PMC346676  PMID: 6956869

Abstract

Nine antigens systems were defined. Two were related to HLA-A,B,C and to Ia-like antigens; the others could be grouped into three categories. (i) NL-22, NL-1: NL-22 antibody reacted with leukemia cells from 12 to 16 cases of null cell acute lymphocytic leukemia (null-ALL) but not with any other type of leukemia tested or with lymphoid cells of various origins. Among cultured cell lines tested, one (NALM-6) of three null-ALL cell lines was positive, the others were negative. Absorption analysis confirmed the restriction of NL-22 antigen to null-ALL. NL-1 antibody was reactive with leukemia cells from 10 to 16 cases of null-ALL and 3 of 6 cases of chronic myelocytic leukemia in blastic crisis (CML-BC). The antigen was present also on a minor population of normal lymphoid cells. The distribution and molecular weight (100,000; glycoprotein) of the NL-1 antigen resembled that of the previously described common ALL antigen (cALL). (ii) NL-30, NL-4: Both antibodies exhibited almost identical patterns of reactivities against cultured cell lines tested. They reacted with leukemia cells from some cases of null-ALL, adult T-cell leukemia, and CML-BC, although they showed discordance in their reactivities against a panel of leukemia cells, (iii) NL-9, NL-8, NL-25: These three antibodies detect serologically distinguishable determinants on a broad range of leukemias and normal lymphoid and hematopoietic cell types. The antibodies analyzed in this study provide evidence for the heterogeneity of null-ALL by demonstrating a variety of antigen phenotypes on leukemia cells. One of the antigens (NL-22) appears to be restricted to null-ALL.

Full text

PDF
4386

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Billing R., Minowada J., Cline M., Clark B., Lee K. Acute lymphocytic leukemia-associated cell membrane antigen. J Natl Cancer Inst. 1978 Aug;61(2):423–429. [PubMed] [Google Scholar]
  2. Borella L., Sen L., Casper J. T. Acute lymphoblastic leukemia (ALL) antigens detected with antisera to E rosette-froming and non-E rosette-forming ALL blasts. J Immunol. 1977 Jan;118(1):309–315. [PubMed] [Google Scholar]
  3. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  4. Chechik B. E., Minowada J. Quantitation of human thymus-leukemia-associated antigen in established hematopoietic cell lines by radioimmunoassay. J Natl Cancer Inst. 1979 Sep;63(3):609–614. doi: 10.1093/jnci/63.3.609. [DOI] [PubMed] [Google Scholar]
  5. Greaves M. F., Brown G., Rapson N. T., Lister T. A. Antisera to acute lymphoblastic leukemia cells. Clin Immunol Immunopathol. 1975 May;4(1):67–84. doi: 10.1016/0090-1229(75)90041-0. [DOI] [PubMed] [Google Scholar]
  6. Greaves M. F., Verbi W., Kemshead J., Kennett R. A monoclonal antibody identifying a cell surface antigen shared by common acute lymphoblastic leukemias and B lineage cells. Blood. 1980 Dec;56(6):1141–1144. [PubMed] [Google Scholar]
  7. Greaves M., Janossy G. Patterns of gene expression and the cellular origins of human leukaemias. Biochim Biophys Acta. 1978 Oct 27;516(2):193–230. doi: 10.1016/0304-419x(78)90008-2. [DOI] [PubMed] [Google Scholar]
  8. Haynes B. F., Metzgar R. S., Minna J. D., Bunn P. A. Phenotypic characterization of cutaneous T-cell lymphoma. Use of monoclonal antibodies to compare with other malignant T cells. N Engl J Med. 1981 May 28;304(22):1319–1323. doi: 10.1056/NEJM198105283042202. [DOI] [PubMed] [Google Scholar]
  9. Kabisch H., Arndt R., Becker W. M., Thiele H. G., Landbeck G. Serological detection and partial characterization of the common-ALL-cell associated antigen in the serum of cALL-patients. Leuk Res. 1979;3(2):83–91. doi: 10.1016/0145-2126(79)90066-3. [DOI] [PubMed] [Google Scholar]
  10. Kennett R. H., Gilbert F. Hybrid myelomas producing antibodies against a human neuroblastoma antigen present on fetal brain. Science. 1979 Mar 16;203(4385):1120–1121. doi: 10.1126/science.424740. [DOI] [PubMed] [Google Scholar]
  11. Kersey J. H., LeBien T. W., Abramson C. S., Newman R., Sutherland R., Greaves M. P-24: a human leukemia-associated and lymphohemopoietic progenitor cell surface structure identified with monoclonal antibody. J Exp Med. 1981 Mar 1;153(3):726–731. doi: 10.1084/jem.153.3.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  13. LeBien T. W., Hurwitz R. L., Kersey J. H. Characterization of a xenoantiserum produced against three molar KCl-solubilized antigens obtained from a non-T, non-B (pre-B) acute lymphoblastic leukemia cell line. J Immunol. 1979 Jan;122(1):82–88. [PubMed] [Google Scholar]
  14. Metzgar R. S., Borowitz M. J., Jones N. H., Dowell B. L. Distribution of common acute lymphoblastic leukemia antigen in nonhematopoietic tissues. J Exp Med. 1981 Oct 1;154(4):1249–1254. doi: 10.1084/jem.154.4.1249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Metzgar R. S., Mohanakumar T. Tumor-associated antigens of human leukemia cells. Semin Hematol. 1978 Apr;15(2):139–156. [PubMed] [Google Scholar]
  16. Miyoshi I., Hiraki S., Tsubota T., Kubonishi I., Matsuda Y., Nakayama T., Kishimoto H., Kimura I., Masuji H. Human B cell, T cell and null cell leukaemic cell lines derived from acute lymphoblastic leukaemias. Nature. 1977 Jun 30;267(5614):843–844. doi: 10.1038/267843a0. [DOI] [PubMed] [Google Scholar]
  17. Pesando J. M., Ritz J., Lazarus H., Costello S. B., Sallan S., Schlossman S. F. Leukemia-associated antigens in ALL. Blood. 1979 Dec;54(6):1240–1248. [PubMed] [Google Scholar]
  18. Pesando J. M., Ritz J., Levine H., Terhorst C., Lazarus H., Schlossman S. F. Human leukemia-associated antigen: relation to a family of surface glycoproteins. J Immunol. 1980 Jun;124(6):2794–2799. [PubMed] [Google Scholar]
  19. Pfreundschuh M., Shiku H., Takahashi T., Ueda R., Ransohoff J., Oettgen H. F., Old L. J. Serological analysis of cell surface antigens of malignant human brain tumors. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5122–5126. doi: 10.1073/pnas.75.10.5122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ritz J., Nadler L. M., Bhan A. K., Notis-McConarty J., Pesando J. M., Schlossman S. F. Expression of common acute lymphoblastic leukemia antigen (CALLA) by lymphomas of B-cell and T-cell lineage. Blood. 1981 Sep;58(3):648–652. [PubMed] [Google Scholar]
  21. Ritz J., Pesando J. M., Notis-McConarty J., Lazarus H., Schlossman S. F. A monoclonal antibody to human acute lymphoblastic leukaemia antigen. Nature. 1980 Feb 7;283(5747):583–585. doi: 10.1038/283583a0. [DOI] [PubMed] [Google Scholar]
  22. Shiku H., Takahashi T., Oettgen H. F. Cell surface antigens of human malignant melanoma. II. Serological typing with immune adherence assays and definition of two new surface antigens. J Exp Med. 1976 Oct 1;144(4):873–881. doi: 10.1084/jem.144.4.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tachibana T., Ishikawa M. A new micro-method for quantitation of human T- and B-lymphocytes. Jpn J Exp Med. 1973 Jun;43(3):227–230. [PubMed] [Google Scholar]
  24. Uchiyama T., Yodoi J., Sagawa K., Takatsuki K., Uchino H. Adult T-cell leukemia: clinical and hematologic features of 16 cases. Blood. 1977 Sep;50(3):481–492. [PubMed] [Google Scholar]
  25. Ueda R., Ogata S., Morrissey D. M., Finstad C. L., Szkudlarek J., Whitmore W. F., Oettgen H. F., Lloyd K. O., Old L. J. Cell surface antigens of human renal cancer defined by mouse monoclonal antibodies: identification of tissue-specific kidney glycoproteins. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5122–5126. doi: 10.1073/pnas.78.8.5122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Winchester R. J., Wang C. Y., Gibofsky A., Kunkel H. G., Lloyd K. O., Old L. J. Expression of Ia-like antigens on cultured human malignant melanoma cell lines. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6235–6239. doi: 10.1073/pnas.75.12.6235. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES