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. 2012 Sep 12;51(40):7917–7929. doi: 10.1021/bi300646y

Figure 2.

Figure 2

Interaction between CaM and GST-tagged CaMBDs of AC1 and AC8. Binding of CaM to GST (26 kDa), the N-terminus of AC8 (GST-8Nt, 34.5 kDa), the C2b domain of AC8 (GST-8C2b, 33.5 kDa), and the C1b domain of AC1 (GST-1C1b, 33.3 kDa) was assessed by a GST pull-down assay with either 200 μM EGTA (−Ca2+) or 20 μM Ca2+ (+Ca2+). Inclusion of 0.5 μM CaMWT is indicated as +CaM. The horizontal lines indicate CaM input (1%) or different GST fusion proteins used in the assay. The top blot shows the expression of GST, and the bottom blot shows the CaM pulled down. A representative experiment of three is shown.