Table 1. Ca²⁺ Dissociation Kinetics of CaM^a.

	koff1 (s–1)	amplitude 1	koff2 (s–1)	amplitude 2
CaM34	>500	(1)	195 ± 29.7	0.030 ± 0.009 (1)
with AC1-C1b	>500	(1)	5.55 ± 0.63	0.032 ± 0.007 (1)
with AC8-Nt	>500	(1)	66.9 ± 10.4	0.027 ± 0.004 (1)
with AC8-C2b	3.46 ± 0.61	0.049 ± 0.006 (2)
CaM12	8.12 ± 0.23	0.061 ± 0.003 (2)
with AC1-C1b	5.17 ± 1.04	0.022 ± 0.010 (1)	0.84 ± 0.27	0.012 ± 0.003 (0.5)
with AC8-Nt	4.18 ± 1.04	0.017 ± 0.002 (0.5)	0.29 ± 0.06	0.022 ± 0.06 (1)
with AC8-C2b	8.45 ± 1.15	0.043 ± 0.007 (1.5)	3.84 ± 1.27	0.021 ± 0.001 (0.5)
CaMWT	7.95 ± 0.29	0.064 ± 0.016 (2)
with AC1-C1b	7.98 ± 0.55	0.053 ± 0.001 (2)	1.16 ± 0.29	0.024 ± 0.006 (1)
with AC8-Nt	4.93 ± 0.86	0.029 ± 0.008 (1)	0.49 ± 0.20	0.027 ± 0.007 (1)
with AC8-C2b	8.38 ± 1.08	0.054 ± 0.006 (2)	1.59 ± 0.58	0.053 ± 0.010 (2)

^aCa²⁺ dissociation kinetics were determined by measuring changes in quin-2 fluorescence at 22 °C and fit with a single- or double-exponential function. The average rate constants for Ca²⁺ dissociation and the amplitude are presented as means ± SD of three to eight independent experiments. A change in the relative amplitude of 0.03 corresponds to a release of one Ca²⁺ ion, and the number of Ca²⁺ ions released is shown in parentheses. The rate constant for Ca²⁺ dissociation, which was too fast to measure, is shown as >500.