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. 1982 Aug;79(15):4575–4579. doi: 10.1073/pnas.79.15.4575

Human somatostatin I: sequence of the cDNA.

L P Shen, R L Pictet, W J Rutter
PMCID: PMC346717  PMID: 6126875

Abstract

RNA has been isolated from a human pancreatic somatostatinoma and used to prepare a cDNA library. After prescreening, clones containing somatostatin I sequences were identified by hybridization with an anglerfish somatostatin I-cloned cDNA probe. From the nucleotide sequence of two of these clones, we have deduced an essentially full-length mRNA sequence, including the preprosomatostatin coding region, 105 nucleotides from the 5' untranslated region and the complete 150-nucleotide 3' untranslated region. The coding region predicts a 116-amino acid precursor protein (Mr, 12.727) that contains somatostatin-14 and -28 at its COOH terminus. The predicted amino acid sequence of human somatostatin-28 is identical to that of somatostatin-28 isolated from the porcine and ovine species. A comparison of the amino acid sequences of human and anglerfish preprosomatostatin I indicated that the COOH-terminal region encoding somatostatin-14 and the adjacent 6 amino acids are highly conserved, whereas the remainder of the molecule, including the signal peptide region, is more divergent. However, many of the amino acid differences found in the pro region of the human and anglerfish proteins are conservative changes. This suggests that the propeptides have a similar secondary structure, which in turn may imply a biological function for this region of the molecule.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. C., Dixon J. E. Isolation and structure of a peptide hormone predicted from a mRNA sequence. A second somatostatin from the catfish pancreas. J Biol Chem. 1981 Aug 25;256(16):8267–8270. [PubMed] [Google Scholar]
  2. Austen B. M. Predicted secondary structures of amino-terminal extension sequences of secreted proteins. FEBS Lett. 1979 Jul 15;103(2):308–313. doi: 10.1016/0014-5793(79)81351-4. [DOI] [PubMed] [Google Scholar]
  3. Bailey J. M., Davidson N. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976 Jan;70(1):75–85. doi: 10.1016/s0003-2697(76)80049-8. [DOI] [PubMed] [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  5. Cooke N. E., Coit D., Shine J., Baxter J. D., Martial J. A. Human prolactin. cDNA structural analysis and evolutionary comparisons. J Biol Chem. 1981 Apr 25;256(8):4007–4016. [PubMed] [Google Scholar]
  6. Cooke N. E., Coit D., Weiner R. I., Baxter J. D., Martial J. A. Structure of cloned DNA complementary to rat prolactin messenger RNA. J Biol Chem. 1980 Jul 10;255(13):6502–6510. [PubMed] [Google Scholar]
  7. Dodd J., Kelly S. Is somatostatin an excitatory transmitter in the hippocampus? Nature. 1978 Jun 22;273(5664):674–675. doi: 10.1038/273674a0. [DOI] [PubMed] [Google Scholar]
  8. Esch F., Böhlen P., Ling N., Benoit R., Brazeau P., Guillemin R. Primary structure of ovine hypothalamic somatostatin-28 and somatostatin-25. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6827–6831. doi: 10.1073/pnas.77.11.6827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Glisin V., Crkvenjakov R., Byus C. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry. 1974 Jun 4;13(12):2633–2637. doi: 10.1021/bi00709a025. [DOI] [PubMed] [Google Scholar]
  10. Hobart P., Crawford R., Shen L., Pictet R., Rutter W. J. Cloning and sequence analysis of cDNAs encoding two distinct somatostatin precursors found in the endocrine pancreas of anglerfish. Nature. 1980 Nov 13;288(5787):137–141. doi: 10.1038/288137a0. [DOI] [PubMed] [Google Scholar]
  11. Hubbard S. C., Ivatt R. J. Synthesis and processing of asparagine-linked oligosaccharides. Annu Rev Biochem. 1981;50:555–583. doi: 10.1146/annurev.bi.50.070181.003011. [DOI] [PubMed] [Google Scholar]
  12. Krejs G. J., Orci L., Conlon J. M., Ravazzola M., Davis G. R., Raskin P., Collins S. M., McCarthy D. M., Baetens D., Rubenstein A. Somatostatinoma syndrome. Biochemical, morphologic and clinical features. N Engl J Med. 1979 Aug 9;301(6):285–292. doi: 10.1056/NEJM197908093010601. [DOI] [PubMed] [Google Scholar]
  13. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  14. Nakanishi S., Inoue A., Kita T., Nakamura M., Chang A. C., Cohen S. N., Numa S. Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nature. 1979 Mar 29;278(5703):423–427. doi: 10.1038/278423a0. [DOI] [PubMed] [Google Scholar]
  15. Noe B. D., Fletcher D. J., Spiess J. Evidence for the existence of a biosynthetic precursor for somatostatin. Diabetes. 1979 Aug;28(8):724–730. doi: 10.2337/diab.28.8.724. [DOI] [PubMed] [Google Scholar]
  16. Oyama H., Bradshaw R. A., Bates O. J., Permutt A. Amino acid sequence of catfish pancreatic somatostatin I. J Biol Chem. 1980 Mar 25;255(6):2251–2254. [PubMed] [Google Scholar]
  17. Patzelt C., Tager H. S., Carroll R. J., Steiner D. F. Identification of prosomatostatin in pancreatic islets. Proc Natl Acad Sci U S A. 1980 May;77(5):2410–2414. doi: 10.1073/pnas.77.5.2410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pradayrol L., Jörnvall H., Mutt V., Ribet A. N-terminally extended somatostatin: the primary structure of somatostatin-28. FEBS Lett. 1980 Jan 1;109(1):55–58. doi: 10.1016/0014-5793(80)81310-x. [DOI] [PubMed] [Google Scholar]
  19. Rorstad O. P., Epelbaum J., Brazeau P., Martin J. B. Chromatographic and biological properties of immunoreactive somatostatin in hypothalamic and extrahypothalamic brain regions of the rat. Endocrinology. 1979 Nov;105(5):1083–1092. doi: 10.1210/endo-105-5-1083. [DOI] [PubMed] [Google Scholar]
  20. Schally A. V., Huang W. Y., Chang R. C., Arimura A., Redding T. W., Millar R. P., Hunkapiller M. W., Hood L. E. Isolation and structure of pro-somatostatin: a putative somatostatin precursor from pig hypothalamus. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4489–4493. doi: 10.1073/pnas.77.8.4489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Srikant C. B., Patel Y. C. Receptor binding of somatostatin-28 is tissue specific. Nature. 1981 Nov 19;294(5838):259–260. doi: 10.1038/294259a0. [DOI] [PubMed] [Google Scholar]
  22. Taylor W. L., Collier K. J., Deschenes R. J., Weith H. L., Dixon J. E. Sequence analysis of a cDNA coding for a pancreatic precursor to somatostatin. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6694–6698. doi: 10.1073/pnas.78.11.6694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]

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