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. 1982 Aug;79(15):4594–4598. doi: 10.1073/pnas.79.15.4594

Site-directed psoralen crosslinking of DNA.

W A Saffran, M Goldenberg, C R Cantor
PMCID: PMC346721  PMID: 6289307

Abstract

A technique has been developed for placing site-specific crosslinks into DNA by using a psoralen derivative containing a sulfhydryl group. Plasmid pBR322 was nicked at the unique BamHI restriction site, and mercurated pyrimidines were introduced by nick-translation. Then the psoralen was specifically directed to this site in the dark through a Hg-S linkage prior to irradiation to form a crosslink. The location of interstrand cross-links was examined by electron microscopy of Pst I-cut molecules and found to be at or near the BamHI site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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