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. 1982 Aug;79(15):4691–4695. doi: 10.1073/pnas.79.15.4691

Chromosomal mapping of the simian sarcoma virus onc gene analogue in human cells.

D C Swan, O W McBride, K C Robbins, D A Keithley, E P Reddy, S A Aaronson
PMCID: PMC346742  PMID: 6289313

Abstract

The primate cell-derived transforming gene (v-sis) of simian sarcoma virus (SSV) is represented as a single copy marker within cellular DNAs of mammalian species including human. The human analogue of v-sis can be distinguished from its rodent counterparts by Southern blotting analysis of EcoRI-restricted DNAs. By testing for the presence of the human v-sis-related fragment, c-sis (human), in somatic cell hybrids possessing varying numbers of human chromosome, as well as in segregants of such hybrids, it was possible to assign c-sis to human chromosome 22.

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