Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Aug;79(15):4761–4765. doi: 10.1073/pnas.79.15.4761

IgG2a monoclonal antibodies inhibit human tumor growth through interaction with effector cells.

D Herlyn, H Koprowski
PMCID: PMC346757  PMID: 6289317

Abstract

Monoclonal antibodies of IgG2a isotype specifically inhibited growth of human tumors in nude mice. Twenty-three monoclonal antibodies of other isotypes showed no tumoricidal reactivity. Complement depletion of nude mice had no effect on tumor suppression by monoclonal antibody. The role of T and killer cells as mediators of the monoclonal antibody effect in nude mice was virtually excluded. On the other hand, macrophages were strongly incriminated as effector cells because silica treatment of nude mice abolished the tumoricidal effect of monoclonal antibody. IgG2a monoclonal antibody-dependent macrophage-mediated cytotoxicity assays with human tumor cells in culture resulted in specific lysis of tumor cells.

Full text

PDF
4761

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernstein I. D., Tam M. R., Nowinski R. C. Mouse leukemia: therapy with monoclonal antibodies against a thymus differentiation antigen. Science. 1980 Jan 4;207(4426):68–71. doi: 10.1126/science.6965328. [DOI] [PubMed] [Google Scholar]
  2. Boltz-Nitulescu G., Bazin H., Spiegelberg H. L. Specificity of fc receptors for IgG2a, IgG1/IgG2b, and IgE on rat macrophages. J Exp Med. 1981 Aug 1;154(2):374–384. doi: 10.1084/jem.154.2.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boltz-Nitulescu G., Foerster O. Antigenic differences between alveolar and peritoneal macrophages of the rat. Lack of population-specific determinants. Immunology. 1979 Nov;38(3):621–630. [PMC free article] [PubMed] [Google Scholar]
  4. Herlyn D. M., Koprowski H. Monoclonal anticolon carcinoma antibodies in complement-dependent cytotoxicity. Int J Cancer. 1981 Jun 15;27(6):769–774. doi: 10.1002/ijc.2910270607. [DOI] [PubMed] [Google Scholar]
  5. Herlyn D. M., Steplewski Z., Herlyn M. F., Koprowski H. Inhibition of growth of colorectal carcinoma in nude mice by monoclonal antibody. Cancer Res. 1980 Mar;40(3):717–721. [PubMed] [Google Scholar]
  6. Herlyn D., Herlyn M., Steplewski Z., Koprowski H. Monoclonal antibodies in cell-mediated cytotoxicity against human melanoma and colorectal carcinoma. Eur J Immunol. 1979 Aug;9(8):657–659. doi: 10.1002/eji.1830090817. [DOI] [PubMed] [Google Scholar]
  7. Herlyn M., Clark W. H., Jr, Mastrangelo M. J., Guerry D. P., 4th, Elder D. E., LaRossa D., Hamilton R., Bondi E., Tuthill R., Steplewski Z. Specific immunoreactivity of hybridoma-secreted monoclonal anti-melanoma antibodies to cultured cells and freshly derived human cells. Cancer Res. 1980 Oct;40(10):3602–3609. [PubMed] [Google Scholar]
  8. Herlyn M., Sears H. F., Steplewski Z., Koprowski H. Monoclonal antibody detection of a circulating tumor-associated antigen. I. Presence of antigen in sera of patients with colorectal, gastric, and pancreatic carcinoma. J Clin Immunol. 1982 Apr;2(2):135–140. doi: 10.1007/BF00916897. [DOI] [PubMed] [Google Scholar]
  9. Herlyn M., Steplewski Z., Herlyn D., Koprowski H. Colorectal carcinoma-specific antigen: detection by means of monoclonal antibodies. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1438–1442. doi: 10.1073/pnas.76.3.1438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kirch M. E., Hammerling U. Immunotherapy of murine leukemias by monoclonal antibody. I. Effect of passively administered antibody on growth of transplanted tumor cells. J Immunol. 1981 Aug;127(2):805–810. [PubMed] [Google Scholar]
  11. Koprowski H., Steplewski Z., Herlyn D., Herlyn M. Study of antibodies against human melanoma produced by somatic cell hybrids. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3405–3409. doi: 10.1073/pnas.75.7.3405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koprowski H., Steplewski Z., Mitchell K., Herlyn M., Herlyn D., Fuhrer P. Colorectal carcinoma antigens detected by hybridoma antibodies. Somatic Cell Genet. 1979 Nov;5(6):957–971. doi: 10.1007/BF01542654. [DOI] [PubMed] [Google Scholar]
  13. Langlois A. J., Matthews T., Roloson G. J., Thiel H. J., Collins J. J., Bolognesi D. P. Immunologic control of the ascites form of murine adenocarcinoma 755. V. Antibody-directed macrophages mediate tumor cell destruction. J Immunol. 1981 Jun;126(6):2337–2341. [PubMed] [Google Scholar]
  14. Mantovani A., Jerrells T. R., Dean J. H., Herberman R. B. Cytolytic and cytostatic activity on tumor cells of circulating human monocytes. Int J Cancer. 1979 Jan 15;23(1):18–27. doi: 10.1002/ijc.2910230105. [DOI] [PubMed] [Google Scholar]
  15. Mazauric T., Mitchell K. F., Letchworth G. J., 3rd, Koprowski H., Steplewski Z. Monoclonal antibody-defined human lung cell surface protein antigens. Cancer Res. 1982 Jan;42(1):150–154. [PubMed] [Google Scholar]
  16. Mitchell K. F., Ward F. E., Koprowski H. DR antigens on melanoma cells: analysis with monoclonal antibodies. Hum Immunol. 1982 Feb;4(1):15–26. doi: 10.1016/0198-8859(82)90046-5. [DOI] [PubMed] [Google Scholar]
  17. Nathan C., Brukner L., Kaplan G., Unkeless J., Cohn Z. Role of activated macrophages in antibody-dependent lysis of tumor cells. J Exp Med. 1980 Jul 1;152(1):183–197. doi: 10.1084/jem.152.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Oldstone M. B., Dixon F. J. Acute viral infection: tissue injury mediated by anti-viral antibody through a complement effector system. J Immunol. 1971 Nov;107(5):1274–1280. [PubMed] [Google Scholar]
  19. Senik A., Gresser I., Maury C., Gidlund M., Orn A., Wigzell H. Enhancement by interferon of natural killer cell activity in mice. Cell Immunol. 1979 Apr;44(1):186–200. doi: 10.1016/0008-8749(79)90039-x. [DOI] [PubMed] [Google Scholar]
  20. Tucker S. B., Pierre R. V., Jordon R. E. Rapid identification of monocytes in a mixed mononuclear cell preparation. J Immunol Methods. 1977;14(3-4):267–269. doi: 10.1016/0022-1759(77)90137-5. [DOI] [PubMed] [Google Scholar]
  21. Wirth J. J., Carney W. P., Wheelock E. F. The effect of particle size on the immunodepressive properties of silica. J Immunol Methods. 1980;32(4):357–373. doi: 10.1016/0022-1759(80)90028-9. [DOI] [PubMed] [Google Scholar]
  22. Young W. W., Jr, Hakomori S. I. Therapy of mouse lymphoma with monoclonal antibodies to glycolipid: selection of low antigenic variants in vivo. Science. 1981 Jan 30;211(4481):487–489. doi: 10.1126/science.7455688. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES